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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (5): 43-48.doi: 10.6040/j.issn.1671-7554.0.2016.492

• 基础医学 • 上一篇    下一篇

有序胶原材料联合CBD-BDNF对大鼠脊髓损伤的修复作用

陈磊1,2,刘东晓1,李开鸣1,宋新强1,2,曾宪思1,2,蒋丽杰1   

  1. 1.信阳师范学院生命科学学院, 河南 信阳 464000;2.信阳师范学院大别山农业生物资源保护与利用研究院, 河南 信阳 464000
  • 收稿日期:2016-05-03 出版日期:2017-05-10 发布日期:2017-05-10
  • 通讯作者: 陈磊. E-mail:chenl_6789@163.com E-mail:chenl_6789@163.com
  • 基金资助:
    国家自然科学基金(81502312、U1204326);信阳师范学院博士科研启动基金(0201305);信阳师范学院2013年青年基金(2013-QN-068)

Uniaxial-channel collagen scaffolds loaded with CBD-BDNF improves the recovery of rat spinal cord injury

CHEN Lei1,2, LIU Dongxiao1, LI Kaiming1, SONG Xinqiang1,2, ZENG Xiansi1,2, JIANG Lijie1   

  1. 1. College of Life Science, Xinyang Normal University, Xinyang 464000, Henan, China;
    2. Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, Henan, China
  • Received:2016-05-03 Online:2017-05-10 Published:2017-05-10

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摘要： 目的 联合有序胶原支架材料和胶原结合结构域-脑源性神经营养因子(CBD-BDNF)修复大鼠脊髓损伤。 方法 以大鼠脊髓半横断损伤模型观察有序材料联合CBD-BDNF对脊髓损伤的修复效果。大鼠分为3组:Control组即损伤后自动恢复组、Collagen组即损伤部位植入有序材料组和CBD-BDNF/collagen组(CBC组)即损伤部位植入有序胶原材料联合CBD-BDNF组。通过神经丝免疫荧光染色、BBB评分和斜板实验观察修复效果。 结果 体外实验发现,有序材料能够引导神经元细胞方向性延伸轴突;体内实验发现,CBC组再生神经丝阳性的神经突起数量明显比Control组和Collagen组多(P<0.05),且再生神经丝延伸具有方向性。CBC组大鼠术后9周BBB评分为8(7,13),明显高于Control组［1(1,1)］和Collagen组［2(1,2)］(P<0.05)。CBC组大鼠斜板耐受角度也要高于Control组和Collagen组(P<0.05)。 结论 CBD-BDNF联合有序胶原支架材料促进脊髓损伤部位神经突起再生和方向性延伸,促进大鼠运动功能恢复,为临床上治疗脊髓横断损伤提供一定实验依据。

关键词: 有序材料, 脊髓损伤, 胶原结合结构域-脑源性神经营养因子, BBB评分, 斜板实验

Abstract: Objective To explore the efficacy of uniaxial guidance collagen scaffolds loaded with collagen binding domain-brain derived neurotrophic factor(CBD-BDNF)in the repair of rat spinal cord injury(SCI). Methods Rat hemisection SCI models were established and divided into 3 groups: control group, collagen group, and CBD-BDNF/collagen(CBC)group. Nerve fiber immunofluorescence staining, BBB score, and inclined plate test were adopted to evaluate the repair efficacy. Results Axons grew through collagen scaffolds in linear fashion in vitro. In animal experiments, CBC group displayed more regenerated neurofilament with linear direction than the other two groups(P<0.05). Nine weeks after implantation, the BBB score of CBC group reached 8(7, 13), significantly higher than the control group［1(1, 1)］ and collagen group［2(1, 2)］,(P<0.05). Inclined plane results also showed significant improvement of functional recovery after SCI in CBC group than the other two groups(P<0.05). Conclusion Collagen scaffolds loaded with CBD-BDNF significantly improves the SCI recovery evaluated by the BBB scale, inclined plane test and immunohistochemical staining with antineurofilament antibody, which suggests that uniaxial guidance 山 东 大 学 学 报 (医 学 版)55卷5期 -陈磊,等.有序胶原材料联合CBD-BDNF对大鼠脊髓损伤的修复作用 \=-collagen scaffolds loaded with CBD-BDNF may serve as an effective strategy for SCI repair.

Key words: Spinal cord injury, Collagen binding domain-brain derived neurotrophic factor, BBB score, Inclined plane score, Uniaxial guidance collagen scaffolds

中图分类号: 

  • R651.2
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