山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (4): 44-49.doi: 10.6040/j.issn.1671-7554.0.2016.246
李霞,李理想,李铭,陈飞雪,左秀丽,李延青
LI Xia, LI Lixiang, LI Ming, CHEN Feixue, ZUO Xiuli, LI Yanqing
摘要： 目的 探讨促肾上腺皮质激素释放因子(CRF)对肠道杯状细胞分泌产物的影响。 方法 首先用不同浓度的CRF作用于LS174T细胞以确定最佳作用浓度;用10-10mol/L的CRF作用于LS174T细胞,利用Real-time qPCR、Western blotting、免疫荧光染色和ELISA技术对肠道杯状细胞分泌产物的表达量进行检测。 结果 Real-time qPCR结果显示,与PBS对照组相比,CRF作用6 h后,黏蛋白2(MUC2),岩藻糖基转移酶-2(FUT2)和三叶因子-3(TFF3)的mRNA表达量均下降(P<0.05)。细胞免疫荧光和ELISA的结果显示,CRF作用6 h后,MUC2的蛋白表达量降低(P<0.05)。Western blotting结果显示,CRF作用24 h后,FUT2和TFF3的蛋白表达水平均下降(P<0.05)。 结论 CRF可以通过改变LS174T细胞分泌产物的表达,从而破坏肠黏膜屏障功能。
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