MiR- 498通过下调FOXM1抑制肺腺癌细胞上皮充质细胞转化

doi:10.6040/j.issn.1671-7554.0.2016.515

山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (4): 39-43.doi: 10.6040/j.issn.1671-7554.0.2016.515

MiR- 498通过下调FOXM1抑制肺腺癌细胞上皮充质细胞转化

唐曦¹,胡娅²,徐炎华¹,汪春林¹,邱萍¹,王向辉³

1.华中科技大学同济医学院附属荆州医院肿瘤中心, 湖北荆州 434020;2.长江大学医学院, 湖北荆州 434023;3.华中科技大学同济医学院附属荆州医院胸外科, 湖北荆州 434020

收稿日期:2016-05-09 出版日期:2017-04-10 发布日期:2017-04-10
通讯作者: 胡娅. E-mail:huy12@126.com E-mail:huy12@126.com
基金资助:
湖北省教育厅2013年度科研项目计划(B2013280);2011~2012年度湖北省卫生厅中医药中西医结合科研项目计划(2012Z-Y31)

MiR- 498 inhibits A549 cells EMT by targeting FOXM1

TANG Xi¹, HU Ya², XU Yanhua¹, WANG Chunlin¹, QIU Ping¹, WANG Xianghui³

1. Cancer Center, Jingzhou Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 434020, Hubei, China;
2. Medical College, Yangtze University, Jingzhou 434023, Hubei, China;
3. Department of Cardiothoracic Surgey, Jingzhou Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 434020, Hubei, China

Received:2016-05-09 Online:2017-04-10 Published:2017-04-10

摘要/Abstract

摘要： 目的研究miR- 498对肺腺癌细胞上皮充质细胞转化(EMT)的影响及相关机制。方法转染miR- 498mimic和miR-NC至离体培养的A549细胞中,将细胞分为空白对照组、miR- 498组和空质粒组3组,Transwell法检测细胞迁移侵袭情况,Western blotting检测A549细胞中E-cadherin、fibronectin、vimentin、FOXM1的表达,利用荧光素酶实验验证miR- 498的靶基因;再通过转染过表达FOXM1的质粒至A549细胞,将细胞分为miR- 498组、miR- 498+FOXM1组、miR- 498+空质粒组,检测A549细胞的上述生物学指标。结果与空质粒组相比,转染miR- 498mimic后,A549细胞中E-cadherin表达增加(P=0.001),fibronectin、vimentin、FOXM1表达减少(P均<0.01),A549细胞的迁移能力减少(P=0.001);荧光素酶实验结果显示,miR- 498能够显著降低FOXM1-3'-UTR 质粒的荧光素活性(P=0.001);与miR- 498+空质粒组相比,转染过表达FOXM1质粒后,A549细胞中E-cadherin表达减少(P=0.002),fibronectin、vimentin表达增加(P均<0.001),A549细胞的迁移能力增加(P=0.001)。结论 miR- 498通过下调FOXM1从而抑制A549细胞的EMT。

关键词: miR- 498, 上皮充质细胞转化, 肺腺癌, FOXM1

Abstract: Objective To investigate the effect of miR- 498 on A549 cells epithelial-to-mesenchymal transition(EMT)and its correlated mechanism. Methods After miR- 498 was transfected into A549 cells, the cells were divided into control group, empty vector group and miR- 498 group; Transwell assay was employed to test the migration ability of A549 cells, Western blotting was used to investigate the expressions of E-cadherin, fibronectin, vimentin and FOXM1 in A549 cells. Luciferase assay was used to confirmed whether FOXM1-3'-UTR was the target gene of miR- 498. Then, the cells were divided into miR- 498 group, miR- 498 + FOXM1 group, and miR- 498 + empty plasmid group, and the above biological indicators of A549 cells were detected again. Results Compared with the empty vector group, the expression of E-cadherin was increased(P=0.001), the expression of fibronectin, vimentin and FOXM1were decreased(all P<0.01), and the migration ablitiy of A549 cells was decreased in the miR- 498 group(P=0.001); the Luciferase activity of the FOXM1-3'-UTR plasmid was significantly suppressed by miR- 498(P=0.001); over-expression of FOXM1 could reverse the effect of miR- 498 on A549. Conclusion miR- 498 inhibits A549 cells EMT by down-regulating FOXM1 expression.

Key words: Lung adenocarcinoma, miR- 498, FOXM1, Epithelial-to-mesenchymal transition

中图分类号:

R734.2

唐曦,胡娅,徐炎华,汪春林,邱萍,王向辉. MiR- 498通过下调FOXM1抑制肺腺癌细胞上皮充质细胞转化[J]. 山东大学学报（医学版）, 2017, 55(4): 39-43.

TANG Xi, HU Ya, XU Yanhua, WANG Chunlin, QIU Ping, WANG Xianghui. MiR- 498 inhibits A549 cells EMT by targeting FOXM1[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(4): 39-43.

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MiR- 498通过下调FOXM1抑制肺腺癌细胞上皮充质细胞转化

MiR- 498 inhibits A549 cells EMT by targeting FOXM1

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