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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (4): 39-43.doi: 10.6040/j.issn.1671-7554.0.2016.515

• 基础医学 • 上一篇    下一篇

MiR- 498通过下调FOXM1抑制肺腺癌细胞上皮充质细胞转化

唐曦1,胡娅2,徐炎华1,汪春林1,邱萍1,王向辉3   

  1. 1.华中科技大学同济医学院附属荆州医院肿瘤中心, 湖北 荆州 434020;2.长江大学医学院, 湖北 荆州 434023;3.华中科技大学同济医学院附属荆州医院胸外科, 湖北 荆州 434020
  • 收稿日期:2016-05-09 出版日期:2017-04-10 发布日期:2017-04-10
  • 通讯作者: 胡娅. E-mail:huy12@126.com E-mail:huy12@126.com
  • 基金资助:
    湖北省教育厅2013年度科研项目计划(B2013280);2011~2012年度湖北省卫生厅中医药中西医结合科研项目计划(2012Z-Y31)

MiR- 498 inhibits A549 cells EMT by targeting FOXM1

TANG Xi1, HU Ya2, XU Yanhua1, WANG Chunlin1, QIU Ping1, WANG Xianghui3   

  1. 1. Cancer Center, Jingzhou Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 434020, Hubei, China;
    2. Medical College, Yangtze University, Jingzhou 434023, Hubei, China;
    3. Department of Cardiothoracic Surgey, Jingzhou Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 434020, Hubei, China
  • Received:2016-05-09 Online:2017-04-10 Published:2017-04-10

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摘要： 目的 研究miR- 498对肺腺癌细胞上皮充质细胞转化(EMT)的影响及相关机制。 方法 转染miR- 498mimic和miR-NC至离体培养的A549细胞中,将细胞分为空白对照组、miR- 498组和空质粒组3组,Transwell法检测细胞迁移侵袭情况,Western blotting检测A549细胞中E-cadherin、fibronectin、vimentin、FOXM1的表达,利用荧光素酶实验验证miR- 498的靶基因;再通过转染过表达FOXM1的质粒至A549细胞,将细胞分为miR- 498组、miR- 498+FOXM1组、miR- 498+空质粒组,检测A549细胞的上述生物学指标。 结果 与空质粒组相比,转染miR- 498mimic后,A549细胞中E-cadherin表达增加(P=0.001),fibronectin、vimentin、FOXM1表达减少(P均<0.01),A549细胞的迁移能力减少(P=0.001);荧光素酶实验结果显示,miR- 498能够显著降低FOXM1-3'-UTR 质粒的荧光素活性(P=0.001);与miR- 498+空质粒组相比,转染过表达FOXM1质粒后,A549细胞中E-cadherin表达减少(P=0.002),fibronectin、vimentin表达增加(P均<0.001),A549细胞的迁移能力增加(P=0.001)。 结论 miR- 498通过下调FOXM1从而抑制A549细胞的EMT。

关键词: miR- 498, 上皮充质细胞转化, 肺腺癌, FOXM1

Abstract: Objective To investigate the effect of miR- 498 on A549 cells epithelial-to-mesenchymal transition(EMT)and its correlated mechanism. Methods After miR- 498 was transfected into A549 cells, the cells were divided into control group, empty vector group and miR- 498 group; Transwell assay was employed to test the migration ability of A549 cells, Western blotting was used to investigate the expressions of E-cadherin, fibronectin, vimentin and FOXM1 in A549 cells. Luciferase assay was used to confirmed whether FOXM1-3'-UTR was the target gene of miR- 498. Then, the cells were divided into miR- 498 group, miR- 498 + FOXM1 group, and miR- 498 + empty plasmid group, and the above biological indicators of A549 cells were detected again. Results Compared with the empty vector group, the expression of E-cadherin was increased(P=0.001), the expression of fibronectin, vimentin and FOXM1were decreased(all P<0.01), and the migration ablitiy of A549 cells was decreased in the miR- 498 group(P=0.001); the Luciferase activity of the FOXM1-3'-UTR plasmid was significantly suppressed by miR- 498(P=0.001); over-expression of FOXM1 could reverse the effect of miR- 498 on A549. Conclusion miR- 498 inhibits A549 cells EMT by down-regulating FOXM1 expression.

Key words: Lung adenocarcinoma, miR- 498, FOXM1, Epithelial-to-mesenchymal transition

中图分类号: 

  • R734.2
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