山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (1): 60-62.doi: 10.6040/j.issn.1671-7554.0.2016.1534
李昀,仲万霞,姚宁,胡双纲,刘洪卯
LI Yun, ZHONG Wanxia, YAO Ning, HU Shuanggang, LIU Hongmao
摘要： 目的 探讨女性年龄对胚胎植入前遗传学诊断及筛查(PGD/PGS)结局的影响。 方法 回顾性分析2015年1月1日至12月31日开展PGD/PGS治疗周期的149例患者,按女性年龄将患者分为3组:Ⅰ组10例(<25岁)共36枚囊胚;Ⅱ组94例(25~34岁)共329枚囊胚;Ⅲ组45例(≥35岁)共89枚囊胚。比较各组之间的胚胎正常率和临床妊娠率。 结果 Ⅰ组、Ⅱ组、Ⅲ组的胚胎正常率分别为50.00%、50.15%、29.21%。 Ⅰ组与Ⅲ组的胚胎正常率比较差异有统计学意义(χ=24.855, P<0.05),Ⅱ组与Ⅲ组的胚胎正常率比较差异有统计学意义(χ=212.376, P<0.05)。Ⅰ组、Ⅱ组、Ⅲ组的临床妊娠率分别为83.33%、82.50%、40.00%。 Ⅰ组与Ⅲ组的临床妊娠率比较差异有统计学意义(χ=23.893, P<0.05),Ⅱ组与Ⅲ组的临床妊娠率比较差异有统计学意义(χ=27.510, P<0.05)。 结论 女性年龄对PGD/PGS结局有影响,当女性年龄≥35岁时会明显降低胚胎的正常率和临床妊娠率。
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| [1] Girardet A, Viart V, Plaza S, et al. The improvement of the best practice guidelines for preimplantation genetic diagnosis of cystic fibrosis: toward an international consensus[J]. Eur J Hum Genet, 2016, 24(4): 469-478. [2] Scott RT, Ferry K, Su J, et al. Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study[J]. Fertil Steril, 2012, 97(4): 870-875. [3] 刘茜桐, 田莉, 师娟子, 等. 胚胎植入前遗传学诊断和筛查的研究进展[J]. 中国妇幼健康研究, 2016, 27(1): 123-126. LIU Xitong, TIAN Li, SHI Juanzi, et al. Research progression on preimplantation genetic diagnosis and screening[J]. Chinese Journal of Woman and Child Health Research, 2016, 27(1): 123-126. [4] 张静, 吕睿, 吕永焕, 等. 胚胎植入前遗传学诊断和筛查的研究进展[J]. 国际生殖健康/计划生育杂志, 2015, 34(3): 243-247. ZHANG Jing, LÜ Rui, LÜ Yonghuan, et al. Research progress of preimplantation genetic diagnosis and screening[J]. Journal of International Reproductive Health/Family Planning, 2015, 34(3): 243-247. [5] Tan YQ, Tan K, Zhang SP, et al. Single-nucleotide polymorphism microarray-based preimplantation genetic diagnosis is likely to improve the clinical outcome for translocation carriers[J]. Hum Reprod, 2013, 28(9): 2581-2592. [6] 陈大蔚, 章志国, 邹薇薇, 等. 反复种植失败患者经植入前遗传学筛查获妊娠的研究[J/CD]. 中华临床医师杂志, 2015, 9(6):20-23. CHEN Dawei, ZHZNG Zhiguo, ZOU Weiwei, et al. Blastocyst preimplantation genetic screening(PGS)of a recurrent implantation failure(RIF)couple[J]. Chinese Journal of Clinicians(Electronic Edition), 2015, 9(6):20-23. [7] Schoolcraft WB, Treff NR, Stevens JM, et al. Live birth outcome with trophectoderm biopsy, blastocyst vitrification, and single-nucleotide polymorphism microarray-based comprehensive chromosome screening in infertile patients[J]. Fertil Steril, 2011, 96(3): 638-640. [8] Scott RT, Upham KM, Forman EJ, et al. Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial[J]. Fertil Steril, 2013, 100(3): 697-703. [9] Fragouli E, Alfarawati S, Goodall N, et al. The cytogenetics of polar bodies: insights into female meiosis and the diagnosis of aneuploidy[J]. Mol Hum Reprod, 2011, 17(5): 286-295. [10] Yang Z, Liu J, Collins GS, et al. Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study[J]. Mol Cytogenet, 2012, 5(1): 24. [11] Gardner DK, Sakkas D. Assessment of embryo viability: the ability to select a single embryo for transfer-a review[J]. Placenta, 2003, 24: S5-S12. [12] Hodes-Wertz B, Grifo J, Ghadir S, et al. Idiopathic recurrent miscarriage is caused mostly by aneuploid embryos[J]. FertilSteril, 2012, 98(3): 675-680. [13] Forman EJ, Tao X, Ferry KM, et al. Single embryo transfer with comprehensive chromosome screening results in improved ongoing pregnancy rates and decreased miscarriage rates[J]. Hum Reprod, 2012, 27(4): 1217-1222. [14] Fischer J, Colls P, Escudero T, et al. Preimplantation genetic diagnosis(PGD)improves pregnancy outcome for translocation carriers with a history of recurrent losses[J]. Fertil Steril, 2010, 94(1): 283-289. [15] Kuliev A, Cieslak J, Ilkevitch Y, et al. Chromosomal abnormalities in a series of 6733 human oocytes in preimplantation diagnosis for age-related aneuploidies[J]. Reprod Biomed Online, 2003, 6(1): 54-59. [16] Pellestor F, Andreo B, Arnal F, et al. Maternal aging and chromosomal abnormalities: new data drawn from in vitro unfertilized human oocytes[J]. Hum Genet, 2003, 112(2): 195-203. [17] Forman EJ, Hong KH, Ferry KM, et al. In vitro fertilization with single euploid blastocyst transfer: a randomized controlled trial[J]. Fertil Steril, 2013, 100(1): 100-107. [18] Ata B, Kaplan B, Danzer H, et al. Array CGH analysis shows that aneuploidy is not related to the number of embryos generated[J]. Reprod Biomed Online, 2012, 24(6): 614-620. [19] Franasiak JM, Forman EJ, Hong KH, et al. The nature of aneuploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening[J]. Fertil Steril, 2014, 101(3): 656-663. [20] Tulay P, Gültomruk M, Fndıklı N, et al. PGD management scheme for older females with balanced translocations: do older females have less chance of balanced embryo transfer?[J]. Turk Ger Gynecol Assoc, 2016, 17(2): 91-95. |
| [1] | . 比较基因组杂交技术的改进及其在产前诊断中的应用[J]. 山东大学学报(医学版), 2009, 47(7): 89-92. |
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