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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (8): 28-33.doi: 10.6040/j.issn.1671-7554.0.2015.1172

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IL-25通过nuocyte细胞诱导哮喘小鼠气道重塑

刘清发1,2,王超1,2,孙启晶1,2,宫晓丹1,2,张才擎2   

  1. 1. 山东大学医学院, 山东 济南 250012;2.山东大学附属千佛山医院呼吸内科, 山东 济南 250014
  • 收稿日期:2015-11-26 出版日期:2016-08-10 发布日期:2016-08-10
  • 通讯作者: 张才擎. E-mail:freezcq66@163.com E-mail:freezcq66@163.com
  • 基金资助:
    山东省科技发展计划(2012GSF11830);山东省自然科学基金(ZR2013HM046)

Effect of interleukin-25 on inducing the airway remodeling of asthma mice by nuocyte cells

LIU Qingfa1,2, WANG Chao1,2, SUN Qijing1,2, GONG Xiaodan1,2, ZHANG Caiqing2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Respiratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-11-26 Online:2016-08-10 Published:2016-08-10

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摘要： 目的 探索白细胞介素-25(IL-25)通过nuocyte细胞对哮喘小鼠气道重塑的作用。 方法 30只SPF级BALB/c雌性小鼠随机分为对照小鼠组、哮喘小鼠组、抗IL-25小鼠组,每组10只,鸡清卵蛋白(OVA)诱导哮喘模型。分离培养哮喘小鼠组nuocyte细胞,并分为对照细胞组、IL-25细胞组、抗IL-25细胞组。HE染色观察肺组织变化,流式细胞法计数肺泡灌洗液(BALF)中nuocyte细胞,ELISA法检测BALF及细胞上清液中IL-4、IL-13含量,免疫组化、Western blotting及RT-PCR法检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ胶原蛋白(collagen-Ⅰ)在肺组织中的表达。 结果 与对照小鼠组相比,哮喘小鼠组BALF中IL-4、IL-13表达升高,nuocyte细胞增多,α-SMA和collagen-Ⅰ表达升高(P<0.05),而抗IL-25小鼠组表达无明显变化(P>0.05);与对照细胞组相比,IL-25细胞组上清液IL-4、IL-13表达增高(P<0.05),抗IL-25细胞组上清液IL-4、IL-13表达无明显变化(P>0.05)。 结论 IL-25可能通过nuocyte细胞促使TH2型细胞因子分泌,导致哮喘小鼠气道重塑。

关键词: nuocyte细胞, 白细胞介素-25, 哮喘, 气道重塑

Abstract: Objective To investigate the effects of interleukin-25(IL-25)on airway remodeling of asthma mice by nuocyte cells. Methods Thirty mice were randomly divided into control mouse group, asthma mouse group, and anti-IL-25 mouse group, ten in each group. Nuocyte cells were collected from the spleens and marrows of asthma mice which were induced by ovalbumin(OVA), and diveded into control cell group, IL-25 cell group, and anti-IL-25 cell group according to the different intervention conditions. The lung tissue pathology was observed by HE staining. The expressions of interleukin-4(IL-4)and interleukin-13(IL-13)proteins in bronchoalveolar lavage fluid(BALF)and nuocyte cells supernatant were detected by ELISA. The numbers of nuocyte cells in BALF were detected by flow cytometry. The expressions of α-smooth muscle actin(α-SMA)and I collagen(collagen-I)were detected with immunohistochemistry, real-time PCR and Western blotting. Results Compared with the control mouse group, expressions of IL-4 and IL-13, numbers of nuocyte cells in BALF, expressions of α-SMA and collagen-I increased in asthma mouse group(P<0.05), while those in anti-IL-25 mouse group had no significant differences(P>0.05). Compared with the control cell group, expressions of IL-4 and IL-13 increased significantly in the cells supernatant in IL-25 cell group(P<0.05), while those in anti-IL-25 group had no significant differences(P>0.05). Conclusion IL-25 may promote the secreting of TH2 cytokines by nuocyte cells, thus leads to the airway remodeling of the asthma mice.

Key words: Airway remodeling, Interleukin-25, Asthma, Nuocyte cell

中图分类号: 

  • R562.25
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