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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (11): 25-31.doi: 10.6040/j.issn.1671-7554.0.2014.885

• 基础医学 • 上一篇    下一篇

IgG在喉鳞状细胞癌中的表达及其临床意义

蒋春樊, 张勤, 梁晋军, 王桂英, 付文荣   

  1. 湖北文理学院附属襄阳市中心医院病理科, 湖北 襄阳 441021
  • 收稿日期:2014-12-01 出版日期:2015-11-10 发布日期:2015-11-10
  • 通讯作者: 蒋春樊.E-mail:sinfulduty@sina.com E-mail:sinfulduty@sina.com
  • 基金资助:
    湖北省襄阳市科学技术研究项目(襄科技2014-9号文件第七项)

IgG expression in laryngeal squamous cell carcinoma and its clinical significance

JIANG Chunfan, ZHANG Qin, LIANG Jinjun, WANG Guiying, FU Wenrong   

  1. Department of Pathology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, Hubei, China
  • Received:2014-12-01 Online:2015-11-10 Published:2015-11-10

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摘要： 目的 探讨IgG在喉鳞癌细胞中的表达及其与B淋巴细胞源性IgG的差异性.方法 采用免疫组化、原位杂交法检测IgG在喉鳞癌细胞的表达;激光显微切割结合RT-PCR法检测喉鳞癌细胞表达IgG重链、轻链基因;测序并BLAST比对IgG重链可变区的VDJ重排;分析喉鳞癌表达IgG与临床病理参数的关系.结果 重链Igγ、轻链Igκ和Igλ蛋白在同一癌细胞中都有表达,且主要分布于癌巢的外围;IgG重链恒定区IGHG1基因mRNA的表达与Igγ在癌细胞中共定位;喉鳞癌细胞中IGHG1、Igκ及Igλ基因均有表达;癌细胞中IgG的VDJ重排呈特定的模式,且有少量的突变,突变率为2.03%～3.67%;低分化、Ⅲ-Ⅳ期或有淋巴结转移的病例有更强的IgG表达.结论 喉鳞癌细胞能够表达IgG,其不同于B淋巴细胞源性IgG的VDJ重排机制;喉鳞癌细胞表达的IgG与临床分期、病理分级和淋巴结转移有关.

关键词: 喉鳞状细胞癌, 临床分期, 病理分级, 免疫球蛋白G

Abstract: Objective To investigate the IgG expression in laryngeal squamous cell carcinoma (LSCC), compare it with IgG of B lymphocytic origin, and explore its possible clinical significance. Methods IgG expression in LSCC cells was detected with immunohistochemistry and in situ hybridization. Heavy chains and light chains of IgG were detected with laser guided microdissection coupled with RT-PCR. VDJ rearrangement of IgG heavy chain variable region 1 was assessed with sequencing and BLAST. The correlation between IgG expression and the pathological parameters was assessed by statistics. Results Igγ, Igκ and Igλ were detected in the same cell of LSCC, characteristic of peripheral distribution at cancer nests. IGHG1 mRNA expressions were co-localized with Igγ in the cancer cells. The mRNAs of IGHG1, Igκ and Igλ were detected in LSCC cells by RT-PCR. VDJ rearrangement occurred when the heavy chains were synthesized, but it presented a monotonous pattern and a small quantity of point mutations, the ratios of which were 2.03%-3.67%. IgG expressions were much stronger in the cases of low differentiation, Ⅲ-Ⅳ stages or lymph node metastasis. Conclusion IgG is expressed in LSCC cells, which has unique VDJ rearrangement different from IgG of B lymphocytic origin. IgG derived from LSCC cells is significantly associated with stages, grades and lymph node metastasis.

Key words: Immunoglobulin G, Laryngeal squamous cell carcinoma, Clinical stage, Pathological grade

中图分类号: 

  • R739.02
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