慢性压迫性神经痛大鼠脊髓水平Reelin蛋白表达变化

摘要/Abstract

摘要： 目的观察慢性坐骨神经压迫性损伤疼痛大鼠脊髓水平Reelin的表达变化情况。方法 54只雄性SD大鼠,随机分为假手术组(Sham组,n=12)和坐骨神经慢性压迫性损伤组(CCI组,n=42)。各组大鼠均于术前1 d,术后1、4、7、10、14、21和28 d测量双侧后足底痛行为学指标,包括机械缩足阈值(PWMT)和热缩足潜伏期(PWTL)。逆转录聚合酶链反应(RT-PCR)、免疫印迹法(Western Blot)检测2组术后1、4、7、10、14、21和28 d脊髓背角Reelin mRNA、Reelin蛋白表达量。结果各组大鼠术前PWMT和PWTL差异无统计学意义(P>0.05);坐骨神经结扎后PWMT和PWTL均出现不同程度降低(P<0.01);与Sham组相比,CCI组Reelin mRNA在腰段脊髓背角水平的表达量减少(P<0.05),Reelin 蛋白表达量也相应减少(P<0.05)。结论慢性压迫性神经痛下大鼠脊髓水平Reelin蛋白的表达呈现下调。

关键词: 十二指肠胆汁引流术, 胆道感染, 重型肝炎, 神经痛, Reelin蛋白, 十二指肠镜, 慢性压迫性损伤, 脊髓背角

Abstract: Objective To detect the expression of Reelin in the lumbar spinal cord of a rat model of neuropathic pain induced by chronic constriction injury on sciatic never (CCI). Methods 54 male SD rats were randomly divided into sham operation group (Sham group, n=12) and CCI model group (CCI group, n=42). Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were tested on day 1 before operation and day 1, 4, 7, 10, 14, 17, 21 and 28 after operation. Western blot and RT-PCR were used to detect the protein and mRNA level of Reelin in rat spinal dorsal horn. Results Both PWMT and PWTL have no difference in rats between sham group and CCI group before operation. After sciatic never ligation, both PWMT and PWTL were significantly decreased(P<0.01)while sham operation have no effects on PWMT and PWTL (P>0.05). Compared with sham group, the expression of Reelin mRNA Were significantly decreased in CCI rats(P<0.05),so were the expression of Reelin protein(P<0.05). Conclusion Reelin was decreased in CCI rat spinal dorsal horn.

Key words: Neuropathic pain, Chronic constriction injury, Reelin, Spinal dorsal horn

中图分类号:

R745.4

阳巧云, 李晶金, 李欢颜, 王波, 彭良玉, 旷昕. 慢性压迫性神经痛大鼠脊髓水平Reelin蛋白表达变化[J]. 山东大学学报（医学版）, 2014, 52(S1): 3-7.

YANG Qiao-yun, LI jin-jin, LI Huan-yan, WANG Bo, PENG Liang-yu, KUANG Xin. The expression of spinal Reelin following chronic constriction injury of the sciatic nerve in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(S1): 3-7.

参考文献

[1] Mehra M,Hill K,Nicholl D,Schadrack J.The burden of chronic low back pain with and without a neuropathic component: a healthcare resource use and cost analysis [J].J Med Econ,2012,15(2):245-252.
[2] Campbell J N,Meyer R A.Mechanisms of neuropathic pain [J].Neuron,2006,52(1):77-92.
[3] Pesold C,Liu WS,Guidotti A,Costa E,Caruncho HJ.Cortical bitufted,horizontal,and Martinotti cells preferentially express and secrete reelin into perineuronal nets,nonsynaptically modulating gene expression [J].Proc Natl Acad Sci,1999,96(6):3217-3222.
[4] Teixeira CM,Martin ED,Sahun I,et al.Overexpression of Reelin prevents the manifestation of behavioral phenotypes related to schizophrenia and bipolar disorder [J].Neuropsychopharmacol,2011,36(12):2395-2405.
[5] Villeda SA,Akopians AL,Babayan AH,et al.Absence of Reelin results in altered nociception and aberrant neuronal positioning in the dorsal spinal cord [J].Neuroscience,2006,139(4):1385-1396.
[6] Wang X,Babayan AH,Basbaum AI,et al.Loss of the Reelin-signaling pathway differentially disrupts heat,mechanical and chemical nociceptive processing [J].Neuroscience,2012,226:441-450.
[7] Rogers JT,Rusiana I,Trotter J ,et al.Reelin supplementation enhances cognitive ability,synaptic plasticity,and dendritic spine density [J].Learn Mem,2011,18(9):558-564.
[8] Murat S.Durakoglugil,Ying Chen,et al.Reelin signaling antagonizes β-amyloid at the synapse [J].Proc Natl Acad Sci,2009,106(37):15938-1538.
[9] Beffert U,Weeber EJ,Durudas A,,et al.Modulation of Synaptic Plasticity and Memory by Reelin Involves Differential Splicing of the Lipoprotein Receptor Apoer2 [J].Neuron,2005,47(4):567-579.
[10]Niu S,Renfro A,Quattrocchi C C,et al.Reelin Promotes Hippocampal Dendrite Development through the VLDLR/ApoER2-Dab1 Pathway [J].Neuron,2004,41(1):71-84.
[11]Zimmermann M.Ethical guidelines for investigations of experimental pain in conscious animals [J].Pain,1983,16(2):109-110.
[12]Bennett G J,Xie Y K.A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man [J].Pain,1988,33(1):87-107.
[13]Tal M,Bennett G J.Extra-territorial pain in rats with a peripheral mononeuropathy: mechano-hyperalgesia and mechano-allodynia in the territory of an uninjured nerve [J].Pain,1994,57(3):375-382.
[14]Hargreaves K,Dubner R ,Brown F,et al.A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia [J].Pain,1988,32(1):77-88.
[15]Zhou H Y,Chen S R,Pan H L.Targeting N-methyl-D-aspartate receptors for treatment of neuropathic pain [J].Expert Rev Clin Pharmacol,2011,4(3):379-388.
[16]Li YH,Hart TZ,K Meng.Tonic facilitation of glutamate release by glycine binding sites on presynaptic NR2B-containing NMDA autoreceptors in the rat visual cortex [J].Neuroscience Letters,2008,432 (3):212-216.
[17]Cull-Candy S,Brickley S,Farrant M.NNDA receptor subunits: diversity,development and disease [J].Curt Opin Neurobiol,2001,11 (3):327-335.
[18]McBain CJ,Mayer ML.N-methyl-D-aspartic acid receptor structure and function [J].Physiol Rev,1994,74(3):723-760.
[19]Foster KA,McLaughlin N,Edbauer D,Phillips M,Bolton A,Constantine-Paton M,Sheng M.Distinct roles of NR2A and NR2B cytoplasmic tails in long-term potentiation [J].The Journal of neuroscience : the official journal of the Society for Neuroscience ,2010,30(7):2676-2685.
[20]Yashiro K,Philpot B D.Regulation of NMDA receptor subunit expression and its implications for LTD,LTP,and metaplasticity [J].Neuropharmacology,2008,55(7):1081-1094.
[21]Zhuo M.Plasticity of NMDA receptor NR2B subunit in memory and chronic Pain [J].Molecular brain ,2009,2:4.
[22]Groc L,Choquet D,Stephenson FA,et al.NMDA receptor surface trafficking and synaptic subunit composition are developmentally regulated by the extracellular matrix protein Reelin [J].J Neurosci,2007,27(38):10165-10175.
[23]Campo C G,Sinagra M,Verrier D,et al.Reelin secreted by GABAergic neurons regulates glutamate receptor homeostasis [J].PLoS One,2009,4(5):e5505.
[24]Qiu S,Weeber E J.Reelin signaling facilitates maturation of CA1 glutamatergic synapses [J].J Neurophysiol,2007,97(3):2312-2321.
[25]Panteri R,Mey J,Zhelyaznik N,et al.Reelin is transiently expressed in the peripheral nerve during development and is upregulated following nerve crush [J].Mol Cell Neurosci,2006,32(1-2):133-142.
[26]Zhao S,Chai X,Frotscher M.Balance between neurogenesis and gliogenesis in the adult hippocampus:role for reelin [J].Dev Neurosci,2007,29(1-2):84-90.
[27]Meisner J G,Marsh A D,Marsh D R.Loss of GABAergic interneurons in laminae I-III of the spinal cord dorsal horn contributes to reduced GABAergic tone and neuropathic pain after spinal cord injury [J].J Neurotrauma,2010,27(4):729-737.
[28]Young S.Gwak,Claire E.Hulsebosch.GABA and central neuropathic pain following spinal cord injury [J].Neuropharmacology,2011,799-808.
[29]Ghiani C A,Mattan N S,Nobuta H,et al.Early effects of lipopolysaccharide-induced inflammation on foetal brain development in rat [J].ASN Neuro,2011,3 (4):e68.

相关文章 12

[1]	张斌,张良文,迟令懿,王传伟,朱树干. 显微血管减压术治疗原发性三叉神经痛疗效的相关因素[J]. 山东大学学报（医学版）, 2016, 54(6): 69-72.
[2]	冯子超,王济潍,李超,李卫国,陈腾,马翔宇,徐淑军,李新钢. 单纯神经内镜在桥小脑角区的手术应用[J]. 山东大学学报（医学版）, 2016, 54(10): 71-75.
[3]	王济潍, 李超, 陈腾, 张文华, 李卫国, 马翔宇, 徐淑军, 李新钢. 单纯神经内镜下微血管减压术治疗原发性三叉神经痛[J]. 山东大学学报（医学版）, 2015, 53(4): 83-86.
[4]	周朋哲. 十二指肠镜引导下十二指肠胆汁引流术在重型肝炎伴胆道感染中的应用[J]. 山东大学学报（医学版）, 2014, 52(Z1): 152-153.
[5]	丁超, 胡启雅, 黄海真, 郭亚秋, 裴淼, 朱庆增, 齐峰. 倍他米松二丙酸酯缓释微球对大鼠神经病理性疼痛的镇痛作用及对脊髓细胞外信号调节激酶的影响[J]. 山东大学学报（医学版）, 2014, 52(10): 35-39,44.
[6]	韩杰1,刘亦青1,任妍妍2,栾德恒1,高昆1,解凤阳1. 三叉神经痛大鼠脑内P物质及β-内啡肽的变化[J]. 山东大学学报(医学版), 2013, 51(8): 45-48.
[7]	张良文，王萍，李峰，杨扬，朱树干. 治疗原发性三叉神经痛 (附142例报告)[J]. 山东大学学报(医学版), 2011, 49(3): 99-101.
[8]	张良文，王萍，李峰，杨扬，朱树干. 治疗原发性三叉神经痛 (附142例报告)[J]. 山东大学学报(医学版), 2011, 49(3): 99-101.
[9]	张国转1，梁立双1，于少华2，刘旭东1. 牛痘疫苗致炎兔皮提取物治疗带状疱疹后神经痛的疗效评价[J]. 山东大学学报(医学版), 2010, 48(9): 107-109.
[10]	杨红卫. MAPK在弗司可林诱发的脊髓背角LTP中的作用[J]. 山东大学学报(医学版), 2010, 48(6): 53-56.
[11]	王磊,徐淑军,周茂德,江玉泉. 神经导航下射频热凝治疗三叉神经痛的临床研究[J]. 山东大学学报(医学版), 2007, 45(9): 965-967.
[12]	孙涛,姚尚龙,宋文阁,傅志俭 . 侧脑室注射内吗啡肽-1降低神经病理性疼痛大鼠海马和PAG IL-6mRNA的表达[J]. 山东大学学报(医学版), 2006, 44(10): 1028-1031.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed