蟾毒灵对CAPAN-2胰腺癌细胞增殖和细胞周期的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (11): 37-41.

蟾毒灵对CAPAN-2胰腺癌细胞增殖和细胞周期的影响

李梅影1，李际盛1，于学军1,孙丽美1，王艾君1，刘奇迹2，王秀问1

1.山东大学齐鲁医院肿瘤中心化疗科，济南 250012; 2.山东大学医学院医学遗传学研究所，济南 250012

收稿日期:2013-03-14 出版日期:2013-11-10 发布日期:2013-11-10
通讯作者: 李际盛, E-mail: lijisheng@gmail.com; 于学军, E-mail：yuxuejun99@163.com
基金资助:
国家自然科学基金(81201934); 中国博士后科学基金(2013M531612); 山东大学自主创新基金(2012TS155)

Effects of bufalin on the proliferation and cell cycle of human pancreatic cancer CAPAN-2 cells

LI Mei-ying1, LI Ji-sheng1, YU Xue-jun1, SUN Li-mei1, WANG Ai-jun1, LIU Qi-ji2, WANG Xiu-wen1

1. Department of Chemotherapy, Cancer Center, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Medical Genetics, School of Medicine, Shandong University, Jinan 250012, China

Received:2013-03-14 Online:2013-11-10 Published:2013-11-10

摘要/Abstract

摘要：

目的研究蟾毒灵在胰腺癌CAPAN-2细胞系中的抗肿瘤作用，探讨其在胰腺癌中的抗肿瘤分子机制。方法 CCK-8法测定蟾毒灵对人胰腺癌CAPAN-2细胞增殖的影响；流式细胞术(FCM)检测蟾毒灵对细胞凋亡和细胞周期的影响；Western blotting法检测蟾毒灵作用于CAPAN-2细胞后Caspase-3酶原(pro-caspase-3)、Bcl-2、Bax、CDC25C、cyclinB1、CDC2蛋白表达水平的变化情况。结果蟾毒灵对胰腺癌CAPAN-2细胞有抑制增殖的作用，并呈现出时间和浓度依赖性；蟾毒灵未能诱导CAPAN-2细胞凋亡，但经蟾毒灵处理后，CAPAN-2细胞周期被阻滞在G2/M期；经蟾毒灵处理后，CAPAN-2细胞中CDC25C、cyclinB1、CDC2等关键周期蛋白的表达量显著下降，但是pro-caspase-3、Bcl-2、Bax等凋亡相关蛋白的表达没有发生明显改变。结论蟾毒灵可显著抑制胰腺癌CAPAN-2细胞增殖，通过诱导G2/M细胞周期阻滞而非诱导细胞凋亡，在CAPAN-2细胞中发挥抗肿瘤作用。

关键词: 胰腺癌；蟾毒灵；细胞周期阻滞；细胞凋亡；抗肿瘤作用

Abstract:

Objective To investigate the anti-cancer role of bufalin in human pancreatic cancer CAPAN-2 cells and explore the underlying mechanisms. Methods The proliferation-inhibiting effect of bufalin on CAPAN-2 cells was measured by CCK-8 assay; the cell cycle distribution and apoptosis were detected by flow cytometry (FCM); the protein expression level of pro-caspase-3, Bcl-2, Bax, CDC25C, cyclinB1 and CDC2 were determined by Western blotting analysis. Results Bufalin inhibited the proliferation of CAPAN-2 cells in a time- and dose-dependent manner; bufalin induced G2/M phase arrest in CAPAN-2 cells but failed to induce cell apoptosis; the protein levels of CDC25C, cyclinB1 and CDC2 were dramatically down-regulated while there were no significant changes in pro-caspase-3, Bcl-2 and Bax protein levels. Conclusion Bufalin could inhibit the proliferation of CAPAN-2 cells by inducing G2/M phase arrest instead of apoptosis.

Key words: Pancreatic cancer; Bufalin; Cell cycle arrest; Apoptosis; Anti-caner effect

中图分类号:

R735.9

李梅影1，李际盛1，于学军1,孙丽美1，王艾君1，刘奇迹2，王秀问1. 蟾毒灵对CAPAN-2胰腺癌细胞增殖和细胞周期的影响[J]. 山东大学学报(医学版), 2013, 51(11): 37-41.

LI Mei-ying1, LI Ji-sheng1, YU Xue-jun1, SUN Li-mei1, WANG Ai-jun1, LIU Qi-ji2, WANG Xiu-wen1. Effects of bufalin on the proliferation and cell cycle of human pancreatic cancer CAPAN-2 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(11): 37-41.

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