幼鼠反复癫痫发作致苔藓纤维发芽的动态变化及其与自发性发作的关系

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (11): 16-20.

幼鼠反复癫痫发作致苔藓纤维发芽的动态变化及其与自发性发作的关系

刘菁，李保敏，孙若鹏，张冬青，李军

山东大学齐鲁医院儿科，济南 250012

收稿日期:2013-05-15 出版日期:2013-11-10 发布日期:2013-11-10
通讯作者: 李保敏， E-mail：lbmsqf@163.com
基金资助:
山东省自然科学基金(ZR2009CM072)；山东省科技发展计划项目(2010GSF10201)

Dynamic changes of mossy fiber sprouting caused by seizures in immature rats and its relationship with spontaneous recurrent seizures

LIU Jing, LI Bao-min, SUN Ruo-peng, ZHANG Dong-qing, LI Jun

Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2013-05-15 Online:2013-11-10 Published:2013-11-10

摘要/Abstract

摘要：

目的观察幼鼠反复癫痫发作致苔藓纤维发芽(MFS)的动态变化及其与慢性期反复自发性发作(SRS)的关系。方法将190只幼年期Wistar大鼠随机分为4组：对照组(n=48)、EP1组(n=80)、空白组(n=12)和EP2组(n=50)，应用氯化锂-匹罗卡品致痫幼鼠模型，诱导幼鼠在生后21、25、29d反复癫痫发作，采用Timm染色法观察EP1组幼鼠末次癫痫发作后1、3、7、14、20、30、45、60d海马MFS的动态变化；观察EP2组SRS情况，比较EP2组中出现SRS(EP2-SRS组)和未出现SRS(EP2-非SRS组)的大鼠MFS的差异；采用Nissl染色观察海马神经元坏死及凋亡情况，对CA3、CA1区神经元进行计数，观察SRS对海马神经元的影响。结果 Timm染色显示，与对照组相比，EP1组在反复癫痫发作后14d MFS明显增加，并持续至60d后(P<0.05)，EP2-SRS组与EP2-非SRS组相比无明显差异(P<0.05)。EP2-SRS组与空白组相比，CA3、CA1区神经元损伤明显(P<0.05)。结论幼年反复癫痫发作引起MFS明显增加；在氯化锂-匹罗卡品模型中，MFS可能不是导致SRS的因素。

关键词: 癫痫；氯化锂-匹罗卡品；苔藓纤维发芽；自发性发作

Abstract:

Objective To investigate the changes of mossy fiber sprouting(MFS) in hippocampus of rats which suffered lithium-pilocarpine induced repeatedly seizures, and the correlation between MFS and spontaneous recurrent seizures(SRS). Methods One hundred and ninety immature Wistar rats were randomly divided into four groups: control group(n=48), EP1 group(n=80), blank group(n=12), EP2 group(n=50). Lithium-pilocarpine was used to induce epilepsy model of rats. Rats were given intraperitoneal injections of pilocarpine on P21, P25 and P29 to induce recurrent seizures. The changes of MFS in hippocampus were examined on days 1, 3, 7, 14, 20, 30, 45 and 60 by Timm staining. Rats in EP2 group were observed every day and were subdivided into EP2-SRS and EP2-nonSRS group, then the difference in MFS was compared between the two groups. The necrosis and apoptosis of the neurons in hippocampus were observed and the neurons in CA3 and CA1 areas were counted by Nissl staining, thus the effect of SRS on the hippocampal neurons could be assessed. Results Compared with control group, significant MFS phenomenon was detected from day 14 to day 60 after recurrent seizures in EP1 group(P<0.05). There was no difference in MFS between EP2-SRS and EP2-nonSRS groups. Compared with blank group, prominent neuron loss was observed in CA3 and CA1 regions of the rats with SRS(P<0.05). Conclusion Recurrent seizures in immature rats can cause an increase of MFS in the hippocampus. We suggest a dissociation between SRS and MFS in the lithium-pilocarpine model of epilepsy.

Key words: Epilepsy; Lithium-pilocarpine; Mossy fiber sprouting; Spontaneous recurrent seizures

中图分类号:

R741.02

刘菁，李保敏，孙若鹏，张冬青，李军. 幼鼠反复癫痫发作致苔藓纤维发芽的动态变化及其与自发性发作的关系[J]. 山东大学学报(医学版), 2013, 51(11): 16-20.

LIU Jing, LI Bao-min, SUN Ruo-peng, ZHANG Dong-qing, LI Jun. Dynamic changes of mossy fiber sprouting caused by seizures in immature rats and its relationship with spontaneous recurrent seizures[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(11): 16-20.

参考文献

相关文章 15

[1]	季恩飞1,刘学文2. p38MAPK信号通路抑制剂对NMDA诱导的体外培养的皮层神经元损伤的保护作用及机制[J]. 山东大学学报(医学版), 2013, 51(11): 10-15.
[2]	梁继业1,2,王翠兰1,邓小梅1. 急性单纯疱疹病毒性脑炎脑脊液可溶性凋亡相关因子的测定[J]. 山东大学学报(医学版), 2013, 51(11): 71-73.
[3]	李艳菊，谭淑慧，夏春凤，张镛，杜怡峰，马国诏. NF-κB对Aβ1-42诱导神经元KATP亚基Kir6.2/SUR1表达的影响[J]. 山东大学学报(医学版), 2013, 51(10): 5-9.
[4]	张晓金1，孙高英1，杨玲玲2，郭元芳1，郝秀玉1，郝建荣1,毕文祥1. 人参皂苷Rb1对NEP基因启动子活性的影响[J]. 山东大学学报(医学版), 2013, 51(10): 42-48.
[5]	韩晓娟1，马学强2，杜怡峰1. 海风藤提取物对Aβ寡聚体激活的小胶质细胞释放炎性因子的抑制作用[J]. 山东大学学报(医学版), 2013, 51(5): 6-10.
[6]	亓珅，韩晓娟，王璐，张清华，杜怡峰. 海风藤提取物对激活的小胶质细胞IL-1β和TNF-α表达的影响[J]. 山东大学学报(医学版), 2013, 51(5): 11-14.
[7]	臧静1，张涌2，王滨2，杨绍祥2，李彬2，王建丽1. CD36单核苷酸多态性与老年动脉粥样硬化性脑梗死的相关性[J]. 山东大学学报(医学版), 2013, 51(5): 99-104.
[8]	张焕丽1，崔亚洲2，周小艳2，尚伟1. 人类癫痫脑组织的蛋白质组学研究[J]. 山东大学学报(医学版), 2013, 51(3): 80-84.
[9]	屈传强1，焉传祝2，陈裴3，王翠兰3，郭洪志2. 基质金属蛋白酶在脑白质疏松症血-脑脊液屏障机制中的实验研究[J]. 山东大学学报(医学版), 2012, 50(10): 23-27.
[10]	彭雷1，董海曼1，张静1，暴丽华1，李贵宝1，宋涛1，刘增训2，孙晋浩1，高英茂1. 帕潘立酮对地佐环平损伤脑皮层神经元的保护作用及其机制[J]. 山东大学学报(医学版), 2012, 50(10): 16-.
[11]	赵云霞，张镛，李甲龙，王瑞霞，李彦芬. 丁苯酞拮抗Aβ1-42致原代培养皮层神经元凋亡的保护机制[J]. 山东大学学报(医学版), 2011, 49(11): 34-39.
[12]	谭淑慧1，任晓燕1，李景新2，姚伟2，马国诏1. Aβ1-42对胆碱能神经元KATP通道离子流影响的研究[J]. 山东大学学报(医学版), 2011, 49(3): 18-.
[13]	谭淑慧1，任晓燕1，李景新2，姚伟2，马国诏1. Aβ1-42对胆碱能神经元KATP通道离子流影响的研究[J]. 山东大学学报(医学版), 2011, 49(3): 18-.
[14]	李甲龙1，马国诏1，张镛1，杜怡峰1，赵云霞1，李景新2，刘克敬2. 二氮嗪对Alzheimer病模型大鼠行为学及其相关凋亡因子的影响[J]. 山东大学学报(医学版), 2010, 48(7): 23-26.
[15]	任凯丁岩崔云燕杨玲玲张静崔行. 神经特异性启动子PDGF-EGFP载体的构建及组织表达特异性鉴定[J]. 山东大学学报(医学版), 2009, 47(5): 50-53.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed