关键词: 手足口病；人肠道病毒；实时荧光定量PCR；济南市

Abstract:

Objective  To analyze epidemiological features and etiological characteristics of hand-foot-mouth disease (HFMD) in Jinan in  2012 and provide scientific data for HFMD prevention and control. Methods  The real-time fluorescence quantitative PCR was used to detect human enterovirus type 71 (HEV71), Coxsackievirus A16 (CVA16) and other enterovirus (PE) simultaneously. Results  Of the 859 specimens with hand-foot-mouth disease collected from hospitals (including 46 severe cases), the positive of human enterovirus was 662, accounting for 77.1%. Children infected with HEV71 and CVA16 accounted for 22.1% (190/859) and 41.9% (360/859), respectively. 8 cases (0.9%) were found to have a mixed infection with HEV71 and CVA16, and 104 cases were infected with other enterovirus (12.1%). These cases were mostly occured  in children less than 6 years old. The common cases were mainly infected with CVA16, while HEV71 was the main pathogen in severe cases, and the ages  of the severe cases were  mostly below 3 years old. Conclusion  In 2012, the major pathogen causing HFMD in Jinan was CVA16, and the co-infection of HEV71 and CVA16  existed; while HEV71 was more common in severe cases and the children who suffered were less than 3 years old.

Key words: Hand-foot-mouth disease; Human enterovirus; Real-time fluorescence quantitative PCR； Jinan city