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山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (10): 62-65.

• 临床医学 • 上一篇    下一篇

活体人主动脉组织高迁移率族蛋白B1的表达及其与动脉粥样硬化发病机制的关系

邢姗姗1,巩祖顺2,穆伟2,王东3,宫玉玲4,邢启崇2   

  1. 1.山东中医药大学,济南 250355;2. 山东大学附属千佛山医院心内科,济南 250014;
    3. 山东大学附属千佛山医院心外科,济南 250014;4. 山东大学附属千佛山医院心脏超声科,济南 250014
  • 收稿日期:2013-07-13 出版日期:2013-10-10 发布日期:2013-10-10
  • 通讯作者: 邢启崇,E-mail: xingqichong@163.com
  • 基金资助:

    山东省优秀中青年科学家科研奖励基金(2009BSB01312);山东省科技发展计划项目(2006GG2202014)

Expression of human living aortic high mobility group protein box1 and its relationship with the pathogenesis of atherosclerosis

XING Shan-shan1, GONG Zu-shun2, MU Wei2, WANG Dong3,GONG Yu-ling4, XING Qi-chong2   

  1. 1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China;
     2. Department of Cardiology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
     3. Department of Cardiovascular, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
    4. Department of Ultrasound Heart, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China
  • Received:2013-07-13 Online:2013-10-10 Published:2013-10-10

摘要:

目的  探讨活体人主动脉组织高迁移率族蛋白B1(HMGB1)的表达与动脉粥样硬化发病机制的关系。方法   36例冠状动脉旁路移植术(CABG)主动脉打孔时废弃的主动脉组织为研究组,12名活体肾移植供体者的动脉组织为对照组。两组患者的样本均应用免疫组化法检测动脉组织HMGB1表达;研究组患者术前测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂蛋白(a)[Lp(a)]、载脂蛋白AⅠ(ApoAⅠ)、载脂蛋白B(ApoB)、高敏C-反应蛋白(hs-CRP)、总胆红素(TBIL)、直接胆红素(DBIL)、间接胆红素(IBIL)水平;采用冠状动脉造影、颈动脉超声检测冠状动脉及颈动脉的病变。结果  研究组主动脉组织HMGB1显著表达,而对照组动脉组织HMGB1无表达。研究组患者血清TC、TG、LDL-C、Lp(a)、ApoB、non-HDL-C、VLDL-C、hs-CRP测定值较参考值显著升高(P均<0.01),而血清HDL-C、ApoAⅠ、TBIL、DBIL、IBIL测定值较参考值明显降低(P均<0.01);冠状动脉重度狭窄发生率为86.1%;颈动脉超声检测显示内膜中层厚度均>1.2mm。结论  活体人主动脉组织HMGB1表达显著,表明动脉粥样硬化炎性反应明显;利用CABG主动脉打孔时废弃的主动脉组织为样本,为研究动脉粥样硬化发病机制及干预靶点提供了新的方法和途径。

关键词: 活体;人主动脉;高迁移率族蛋白B1;冠状动脉分流术;动脉粥样硬化

Abstract:

Objective  To investigate the changes of high mobility group box1 (HMGB1) in the aorta of patients with coronary artery disease and its association with pathogenesis of atherosclerosis. Methods  Aortic tissues from 36 patients undergoing coronary artery bypass graft (CABG) surgery were taken as the study group, while aortic tissues from 12 healthy subjects in the kidney donation program were taken as controls. Expression of HMGB1 was determined by immunohistochemistry. The preoperative plasma TC, TG, LDL-C, HDL-C, Lp (a), ApoA I, ApoB, non-HDL-C, VLDL-C, hs-CRP, TBIL, DBIL and IBIL of patients in the research group were examined. The coronary angiography and carotid artery ultrasound were performed in order to analyze the severity of atherosclerotic lesions. Results  The HMGB1 protein was detectable in aortas from CABG patients. However, it was absent from the controlled tissues. The plasma TC, TG, LDL-C, Lp (a), ApoB, non-HDL-C, VLDL-C, and hs-CRP of patients in the research group were significantly elevated than the reference values (P<0.01), whereas plasma HDL-C, ApoA I, TBIL, DBIL and IBIL were significantly lower than the reference values (P<0.01). The incidence of severe coronary artery stenosis was 86.1% and intima-media thickness was more than 1.2mm. Conclusion  Over-expression of HMGB1 in atherosclerosis aortic tissues indicates that inflammatory reaction is obvious in atherosclerosis mottling. Taking aortic tissues from CABG as samples may be a new method in the study of atherosclerosis.

Key words: Living; Human aorta; High mobility group box 1; Coronary artery bypass; Atherosclerosis

中图分类号: 

  • R543.5
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