ALS小鼠脊髓组织GFAP蛋白赖氨酸的乙酰化修饰

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (10): 15-18.

ALS小鼠脊髓组织GFAP蛋白赖氨酸的乙酰化修饰

李俊强1，郭继生1，王小岳1，王道光1，赵作辉2，杨静华1

1.山东大学医学院癌症研究中心，济南 250012； 2.山东大学附属省立医院泌尿外科，济南 250021

收稿日期:2013-03-04 出版日期:2013-10-10 发布日期:2013-10-10
通讯作者: 杨静华， E-mail：jyang@bu.edu

Glial fibrillary acid protein lysine acetylated modification in ALS mouse

LI Jun-qiang1, GUO Ji-sheng1, WANG Xiao-yue1, WANG Dao-guang1, ZHAO Zuo-hui2, YANG Jing-hua1

1. Department of Cancer Research Center, School of Medicine, Shandong University, Jinan 250012, China;
2. Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2013-03-04 Online:2013-10-10 Published:2013-10-10

摘要/Abstract

摘要：

目的探讨肌萎缩脊髓硬化症(ALS)模型小鼠脊髓组织胶质纤维酸性蛋白(GFAP)中赖氨酸的乙酰化修饰。方法对4个月左右的ALS模型小鼠进行麻醉处死，提取小鼠脊髓组织总蛋白，通过GFAP抗体和乙酰化抗体，运用免疫沉淀和免疫蛋白印迹技术鉴定GFAP蛋白的表达与GFAP蛋白赖氨酸的乙酰化修饰，通过液相二级质谱连用(LC-MS/MS)检测ALS模型小鼠脊髓组织中GFAP赖氨酸的乙酰化修饰位点。结果免疫沉淀和免疫蛋白印迹方法发现ALS模型小鼠脊髓组织中GFAP蛋白存在赖氨酸乙酰化修饰；LC-MS/MS发现GFAP的394位和402位赖氨酸位点发生乙酰化修饰。结论 ALS小鼠模型脊髓组织中，GFAP的赖氨酸位点能被乙酰化修饰，该修饰可能参与了ALS的发生。

关键词: 胶质纤维酸性蛋白；乙酰化修饰；肌萎缩脊髓硬化症；液相二级质谱连用

Abstract:

Objective To investigate glial fibrillary acidic protein (GFAP) lysine acetylated modification in the spinal cord tissue of amyotrophic lateral sclerosis (ALS) model mouse. Methods After 3 ALS mice about 4 months old were sacrificed with anesthesia, total protein was extracted by RIPA lysis buffer; GFAP and Pan-AC protein expression were identified by immunoprecipitation and western blotting. Then IP-purified GFAP protein was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results IP-purified GFAP protein from ALS mice was acetylated in lysine residues, and the GFAP 394 and 402 lysine residues were confirmed by LC-MS/MS. Conclusion The lysine residue of GFAP can be modified by acetylation, which may participate in the occurrence of ALS.

Key words: Glial fibrillary acidic protein; Acetylated modification; Amyotrophic lateral sclerosis; LC-MS/MS

中图分类号:

李俊强1，郭继生1，王小岳1，王道光1，赵作辉2，杨静华1. ALS小鼠脊髓组织GFAP蛋白赖氨酸的乙酰化修饰[J]. 山东大学学报(医学版), 2013, 51(10): 15-18.

LI Jun-qiang1, GUO Ji-sheng1, WANG Xiao-yue1, WANG Dao-guang1, ZHAO Zuo-hui2, YANG Jing-hua1. Glial fibrillary acid protein lysine acetylated modification in ALS mouse[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 15-18.

参考文献

相关文章 7

[1]	谭雅文，高海东. USF2与其截短体真核表达载体的构建及对Smurf1/2转录水平的影响[J]. 山东大学学报(医学版), 2013, 51(8): 34-37.
[2]	谢迪东1,2，龚正3，李容2，李慧2，刘宏达2，孙金鹏1,2，庞琦1. 磷酸酶STEP的Q-loop中T541参与催化反应的机制[J]. 山东大学学报(医学版), 2013, 51(8): 38-44.
[3]	于春晓1,2,3，金童4，姜安丽5，赵家军1,2,3. 人同源盒基因NKX3.1内含子及5′上游10kb调控区功能的初步分析[J]. 山东大学学报(医学版), 2012, 50(12): 13-.
[4]	齐晓丽1，田克立1，张典瑞2，徐霞1，冯飞飞2，任桂杰1，苑辉卿1，褚倩倩1，张强3. 冬凌草甲素诱导胰腺癌PANC-1细胞凋亡及对G2/M细胞周期的阻滞作用[J]. 山东大学学报(医学版), 2011, 49(2): 9-13.
[5]	韦超1,吴围屏1,赵燕1,王林1,闫新峰2 ,常晓天1. 雌激素对胶原诱导关节炎动物模型发病过程的作用[J]. 山东大学学报(医学版), 2010, 48(8): 54-59.
[6]	高选1，2，颜军昊2，宦晴2，黄色新2，徐凯2，陈子江2，王磊1. GM1对人胚胎干细胞定向诱导分化神经细胞的作用[J]. 山东大学学报(医学版), 2009, 47(12): 21-24.
[7]	. 脂联素对L6大鼠骨骼肌细胞葡萄糖摄取的影响[J]. 山东大学学报(医学版), 2009, 47(10): 5-8.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed