虫草素对环磷酰胺所致骨髓抑制小鼠CenpB表达的影响

doi:10.6040/j.issn.1671-7554.0.2014.861

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (7): 24-28.doi: 10.6040/j.issn.1671-7554.0.2014.861

虫草素对环磷酰胺所致骨髓抑制小鼠CenpB表达的影响

董佳亮, 张蕾

辽宁医学院基础医学院生化教研室, 辽宁锦州 121000

收稿日期:2014-11-24 修回日期:2015-03-25 出版日期:2015-07-10 发布日期:2015-07-10
通讯作者: 张蕾。E-mail:zhanglei_1962@126.com E-mail:zhanglei_1962@126.com

Effect of cordycepin on the expression of CenpB of myelosuppression mice induced by cyclophosphamide

DONG Jialiang, ZHANG Lei

Department of Biochemistry, Basic Medical College of Liaoning Medical University, Jinzhou 121000, Liaoning, China

Received:2014-11-24 Revised:2015-03-25 Online:2015-07-10 Published:2015-07-10

摘要/Abstract

摘要： 目的探讨虫草素对腹腔注射环磷酰胺所致的小鼠骨髓抑制的治疗作用。方法将30只小鼠随机分为空白对照组(A组)、环磷酰胺骨髓抑制组(B组)和虫草素干预骨髓抑制组(C组), 每组10只。以5 mL/kg剂量向A组小鼠腹腔注射生理盐水12 d;以50 mg/kg剂量向B组小鼠腹腔注射环磷酰胺7 d后, 腹腔注射生理盐水5 d;以50 mg/kg剂量向C组小鼠腹腔注射环磷酰胺7 d后, 以25 mg/kg剂量腹腔注射虫草素5 d。对各组小鼠的外周血细胞以及股骨中的骨髓有核细胞进行计数, 采用Western blotting检测白细胞中的着丝粒蛋白CenpB的蛋白表达水平, 采用RT-PCR检测CenpB的mRNA表达水平。结果与A组相比, B组小鼠外周血白细胞、红细胞、血小板, 股骨中骨髓有核细胞数量, CenpB蛋白相对含量及其表达水平均明显降低(P<0.05);与B组相比, C组中除红细胞数量外, 其他5项指标均明显增加(P<0.05)。结论虫草素对环磷酰胺所致的骨髓抑制有一定治疗作用。

关键词: 环磷酰胺, 虫草素, 骨髓抑制, CenpB蛋白

Abstract: Objective To investigate the effect of cordycepin on myelosuppression mice caused by intraperitoneal injection of cyclophosphamide. Methods Thirty mice were divided into the control group (group A), myelosuppression induced by cyclophosphamide group (group B) and myelosuppression intervened by cordycepin group (group C), 10 in each group. Mice in group A were intraperitoneally injected of normal saline for 12 d with the dosage of 5 mL/kg; Mice in group B were injected of normal saline for 5d after injection of cyclophosphamide for 7 d with the dosage of 50 mg/kg; Mice in group C were injected of cordycepin for 5 d with the dosage of 25 mg/kg after injection of cyclophosphamide for 7 d with the dosage of 50 mg/kg. The numbers of cells in peripheral blood and nucleated cells in femur of each mouse were counted. The content and the expression level of CenpB in leukocytes were detected by Western blotting and RT-PCR. Results Compared with those in group A, the numbers of leukocytes, red blood cells, platelets and nucleated cells, and the content and the expression level of CenpB were significantly decresed (P<0.05). Compared with those in group B, all the indexes, except for the number of red blood cells, were significantly incresed (P<0.05). Conclusion Cordycepin has a certain therapeutic function to myelosuppression mice caused by cyclophosphamide.

Key words: Myelosuppression, Cordycepin, CenpB protein, Cyclophosphamide

中图分类号:

R965.1

董佳亮, 张蕾. 虫草素对环磷酰胺所致骨髓抑制小鼠CenpB表达的影响[J]. 山东大学学报（医学版）, 2015, 53(7): 24-28.

DONG Jialiang, ZHANG Lei. Effect of cordycepin on the expression of CenpB of myelosuppression mice induced by cyclophosphamide[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 24-28.

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虫草素对环磷酰胺所致骨髓抑制小鼠CenpB表达的影响

Effect of cordycepin on the expression of CenpB of myelosuppression mice induced by cyclophosphamide

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