您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (7): 25-27.

• 基础医学 • 上一篇    下一篇

不同浓度谷氨酸钠摄入对Wistar大鼠代谢及胰岛细胞凋亡的影响

张洁1,许翠萍2,徐泽俊1,李燕1,薛秀娟3,刘庆芝3   

  1. 1.山东大学护理学院, 济南 250012; 2.山东大学附属千佛山医院人力资源部, 济南 250014;
    3.泰山医学院研究生部, 山东 泰安  271016
  • 收稿日期:2013-02-26 出版日期:2013-07-10 发布日期:2013-07-10
  • 通讯作者: 许翠萍, E-mail:xucuiping775@sohu.com
  • 基金资助:

    山东省自然科学基金(ZR2010HL007)

Effects of different concentrations of MSG on the metabolism and  islet cell apoptosis in Wistar rats

ZHANG Jie1, XU Cui-ping2, XU Ze-jun1, LI Yan1, XUE Xiu-juan3, LIU Qing-zhi3   

  1. 1. School of Nursing , Shandong University, Jinan 250012, China;  2. Department of Personal Resources,
    Qianfoshan Hospital, Jinan 250014, China;  3. Graduate Faculty of Taishan Medical College, Taian 271016, Shandong China
  • Received:2013-02-26 Online:2013-07-10 Published:2013-07-10

摘要:

目的   探索不同浓度谷氨酸钠(MSG)摄入对大鼠代谢及胰岛细胞凋亡的影响。方法   将40只Wistar 大鼠随机分为对照组、MSG标准剂量组、MSG 4倍标准剂量组、MSG 8倍标准剂量组,每组10只。对照组给予生理盐水灌胃,MSG组给予自配的MSG溶液灌胃,每周空腹取尾静脉血1次,测血糖、甘油三酯、总胆固醇水平,于33周取颈动脉血测其血糖、胰岛素、甘油三酯、总胆固醇水平。结果   不同组间血糖水平差异无统计学意义(P>0.05),但MSG 8倍标准剂量组与其他3组比较,甘油三酯、总胆固醇、胰岛素水平均明显升高,差异有统计学意义(P<0.05),MSG 8倍标准剂量组的胰岛素抵抗指标与其他3组相比,差异有统计学意义(P<0.05),说明该组出现了胰岛素抵抗;各MSG组均造成胰岛细胞的形态学改变,且随着MSG浓度的升高,胰岛细胞的凋亡率也逐渐升高, 8倍MSG剂量组与其他组相比,差异有统计学意义(P<0.05)。结论   高剂量MSG摄入会造成代谢紊乱并损伤胰岛功能,一旦胰岛功能由胰岛素抵抗发展至胰岛失代偿,可能会引发高血糖。

关键词: 谷氨酸钠;血糖;胰岛素;糖尿病

Abstract:

Objective   To explore the effects of different concentrations of monosodium glutamate (MSG) on the metabolism and islet cell apoptosis in rats. Methods   Forty Wistar rats were randomly divided into four groups: the control group, the MSG normal dose group, the MSG four times  dose group and the MSG eight times dose group, each with 10 rats respectively. The rats in the control group and other three groups were administered intragastrically with normal saline and homemade MSG solution, respectively. Fasting glucose, triglycerinate and total cholesterol were examined each week from the aft venous blood and the carotid arterial blood was collected to test fasting glucose,insulin, triglycerinate and total cholesterol at week 33. Results   There was no statistical difference in fasting glucose among the four groups (P>0.05). However, compared with the other three groups, the MSG eight times dose group had higher levels of insulin, triglycerinate and total cholesterol, and the difference had statistical significance (P<0.05). The insulin resistance was also shown in that group (P<0.05). In the MSG groups, all doses of MSG caused the morphological changes of islet cells, and with the increasing of MSG, the apoptotic rates of islet cell also increased. But only the difference between the MSG eight times group and the other three groups had statistical significance (P<0.05).  Conclusion   High dose of MSG could cause metabolic disorders and impair the function of islet. Hyperglycemia would occur if the islet was decompensated.

Key words: Monosodium glutamate; Glucose; Insulin; Diabetes

中图分类号: 

  • R587.1
[1] 武传龙1,许良银2,侯新国1,赵汝星1,陈丽1. 甲状腺功能与肥胖及代谢综合征的相关性分析[J]. 山东大学学报(医学版), 2013, 51(12): 88-91.
[2] 张敏1,耿厚法2,孙琳2. 氢质子磁共振波谱技术对2型糖尿病合并急性脑梗死患者脑内代谢物变化的评估[J]. 山东大学学报(医学版), 2013, 51(11): 74-77.
[3] 李争明,于健. 妊娠相关暴发性1型糖尿病酮症酸中毒并死胎1例[J]. 山东大学学报(医学版), 2013, 51(11): 107-107.
[4] 胡苏1,2,逄曙光2,崔莹2,于春晓1,赵家军1,管庆波1. 链脲佐菌素诱导糖尿病大鼠骨髓间充质干细胞成骨分化[J]. 山东大学学报(医学版), 2013, 51(8): 7-12.
[5] 刘丹丹1,高聆2,赵家军1,韩文霞1. 健脾益肾、活血化瘀中药复方对高糖刺激下肾小球系膜细胞增殖的影响[J]. 山东大学学报(医学版), 2013, 51(5): 29-32.
[6] 李亚男1,蒋玲1,胡秀慧1,张晓黎1,李杰2. 碘酸钾和双酚A对甲状腺乳头状癌细胞增殖的影响[J]. 山东大学学报(医学版), 2013, 51(3): 52-57.
[7] 王小磊1,高聆1,于春晓1,刘元涛2,张楠1,管庆波1,赵家军1. 硫化氢对高糖诱导的血管炎症反应的影响[J]. 山东大学学报(医学版), 2013, 51(2): 44-48.
[8] 于苏国1,王令令2,孙吉花3,王迎雪1,赵慧1. 吡格列酮对高脂饲养诱导胰岛素抵抗大鼠胰腺组织PTP-1B表达的影响[J]. 山东大学学报(医学版), 2013, 51(1): 17-21.
[9] 张秀荣1,2,徐继马1,3,严庆涛1,4,季万胜3,房春燕1,2,王琳1,2,赵廷坤1,2,高志芹1,5,曲梅花1,2. DJB术对2型糖尿病大鼠肠神经GLP-1受体表达影响的研究[J]. 山东大学学报(医学版), 2012, 50(11): 1-5.
[10] 王美建,侯新国,宋君,周克华,杨俊朋,肖芳,刘继东,邓楠,周晓莹,陈丽. 2型糖尿病患者胰岛素治疗结构化管理模式研究[J]. 山东大学学报(医学版), 2012, 50(10): 1-5.
[11] 梁凯1,侯新国1,王新华2,宋允胜2,井庆平3,曲勇4,孙宇1,宋君1,董晓琳1,许聿新1,李振作1,付春莉1,李文娟1,王云霞1,矫磊1,曹立君1,尹小芳1,范琳琳1,陈丽1. 人体肥胖测量参数对糖代谢异常风险的预测价值研究[J]. 山东大学学报(医学版), 2012, 50(9): 59-62.
[12] 刘继东,侯新国,宋君,李文娟,校娟,肖芳,王美建,邓楠,周晓莹,陈丽. 正常高值血压合并糖调节受损患者的心血管危险因素[J]. 山东大学学报(医学版), 2012, 50(7): 1-.
[13] 陈静1,耿厚法2,孙琳2. FTO rs9939609多态性与2型糖尿病合并冠心病的相关性[J]. 山东大学学报(医学版), 2012, 50(7): 10-.
[14] 孙东,李明龙,孙晓静,林爱清,张荣荣,潘翠真. 型糖尿病患者血清褪黑素水平及分泌节律的改变与氧化应激的关系[J]. 山东大学学报(医学版), 2012, 50(6): 10-.
[15] 杜月娟1,刘元涛2,卢圣霞1,刘晔1,齐文文1,傅余芹1. 冬虫夏草菌丝对糖尿病大鼠肾脏DKK1、β-catenin表达的影响[J]. 山东大学学报(医学版), 2012, 50(6): 26-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!