伴13号染色体缺失的多发性骨髓瘤患者CD138+细胞差异表达miRNAs的筛选

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (5): 80-84.

伴13号染色体缺失的多发性骨髓瘤患者CD138+细胞差异表达miRNAs的筛选

翟珊珊1，丁卜同2，李海霞3，陈昀1，常亚丽1，桑坦1，郭农建1

山东大学附属济南市中心医院 1.血液肿瘤科； 2.病理科； 3.保健科，济南 250013

收稿日期:2012-11-21 出版日期:2013-05-10 发布日期:2013-05-10
通讯作者: 郭农建, E-mail:gnjian2002@yahoo.com.cn
基金资助:
济南市科技发展计划(200905061)；山东省医药卫生科技发展计划(2011HW010)；济南市卫生局科技项目(200809)

Differential expressions of microRNAs in the CD138+ cells of multiple myeloma patients with deletion of chromosome 13

ZHAI Shan-shan1, DING Bu-tong2, LI Hai-xia3, CHEN Yun1, CHANG Ya-li1, SANG Tan1, GUO Nong-jian1

1. Department of Hematology and Oncology; 2. Department of Pathology; 3. Department of Health;
Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China

Received:2012-11-21 Online:2013-05-10 Published:2013-05-10

摘要/Abstract

摘要：

目的初步筛选伴13号染色体缺失的多发性骨髓瘤患者CD138+细胞差异表达显著的microRNAs(miRNAs)并探求其生物学功能。方法通过荧光原位杂交(FISH)实验从29例多发性骨髓瘤初治患者中筛选出13号染色体缺失和核型正常的多发性骨髓瘤患者各3例；应用miRNA芯片技术获得差异表达miRNAs，并进行实时定量PCR验证；应用在线软件预测差异miRNAs的潜在靶基因，并建立miRNAs与靶基因的调控网络。结果检测出5个在伴13号染色体缺失的多发性骨髓瘤患者CD138+细胞中差异表达的miRNAs，其中4个表达下调，1个表达上调；生物信息学分析显示，SMAD3基因位于miRNAs靶基因调控网络的关键节点上。结论伴13号染色体缺失的多发性骨髓瘤患者CD138+细胞中存在特异性表达的miRNAs；SMAD3基因可能在该类型多发性骨髓瘤发病中发挥作用。

关键词: 微小RNA；多发性骨髓瘤；芯片；靶基因；SMAD3

Abstract:

Objective To screen the differentially expressed microRNAs in the CD138+ cells of multiple myeloma (MM) patients with deletion of chromosome 13 and explore the biological functions of them. Methods 3 cases with chromosome 13 deletion and another 3 with normal karyotype from 29 previously untreated multiple myeloma patients were screened by fluorescence in situ hybridization (FISH). The miRNA microarray was used to detect the differentially expressed miRNAs, and the real-time PCR was used to verify the results. The potential target genes of the detected differentially expressed miRNAs were predicted by online software. Then the regulatory network of miRNAs and target genes was established. Results Five differentially expressed miRNAs were detected in the CD138+ cells of MM patients with deletion of chromosome 13, of which four were down-regulated and one was up-regulated obviously. Bioinformatic analysis showed that SMAD3 was in the key node of the miRNA-target gene regulatory network. Conclusion The multiple myeloma patients with chromosome 13 deletion have their specifically expressed miRNAs. The gene of SMAD3 may play an important role in the pathogenesis of this type of disease.

Key words: miRNA; Multiple myeloma; Microarray; Target gene；SMAD3

中图分类号:

R551.3

翟珊珊1，丁卜同2，李海霞3，陈昀1，常亚丽1，桑坦1，郭农建1. 伴13号染色体缺失的多发性骨髓瘤患者CD138+细胞差异表达miRNAs的筛选[J]. 山东大学学报(医学版), 2013, 51(5): 80-84.

ZHAI Shan-shan1, DING Bu-tong2, LI Hai-xia3, CHEN Yun1, CHANG Ya-li1, SANG Tan1, GUO Nong-jian1. Differential expressions of microRNAs in the CD138+ cells of multiple myeloma patients with deletion of chromosome 13[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 80-84.

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