眼针对局灶性脑缺血再灌注大鼠VEGF/VEGFR系统表达的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (2): 22-.

眼针对局灶性脑缺血再灌注大鼠VEGF/VEGFR系统表达的影响

宓丹1，2，马贤德3，王建4

辽宁中医药大学 1.研究生部, 沈阳 110032; 2.附属第二医院急诊科, 沈阳 110036; 3.基础部, 沈阳 110032; 4.附属医院神经内科, 沈阳 110032

收稿日期:2012-10-05 出版日期:2013-02-10 发布日期:2013-02-10
通讯作者: 宓丹(1973- )，女，博士研究生，主要从事中医药防治脑血管病研究。 E-mail: 1542844848@qq.com
作者简介:宓丹(1973- )，女，博士研究生，主要从事中医药防治脑血管病研究。 E-mail: 1542844848@qq.com
基金资助:
国家重点基础研究发展计划项目(2007CB512702)

Effects of eye acupuncture on VEGF and VEGFR expressions in focal cerebral ischemia-reperfusion rats

MI Dan1,2, MA Xian-de3, WANG Jian4

1. Graduate Department, Liaoning University of Chinese Medicine, Shenyang 110032， China; 2. Department of Emergency, The Second Hospital Affiliated to Liaoning University of Chinese Medicine, Shenyang 110036， China;3. Basic Department, Liaoning University of Chinese Medicine, Shenyang 110032， China; 4. Department of Neurology, The Affiliated Hospital of Liaoning University of Chinese Medicine, Shenyang 110032， China

Received:2012-10-05 Online:2013-02-10 Published:2013-02-10

摘要/Abstract

摘要：

目的通过观察眼针对局灶性脑缺血再灌注大鼠血管内皮生长因子及其受体(VEGF/VEGFR)系统表达的影响，探讨眼针促进局灶性脑缺血再灌注大鼠微血管生成的作用机制。方法采用改良线栓法制备局灶性脑缺血再灌注模型，采用眼针治疗。再灌注3、24、72h后，免疫组化法检测脑微血管密度及VEGF、VEGFR2表达；逆转录-聚合酶链反应法检测大鼠VEGF mRNA、VEGFR2 mRNA表达。结果与模型组比较，眼针组缺血再灌注24、72h微血管密度明显增加，各时间点VEGF、VEGFR2表达明显增加，VEGF mRNA、VEGFR2 mRNA表达明显上调(P均＜0.05)。结论眼针可以促进脑缺血再灌注大鼠微血管生成，其机制与上调VEGF/VEGFR表达有关。

关键词: 血管新生; 眼针; 血管内皮生长因子; 血管内皮生长因子受体

Abstract:

Objective To explore the mechanism of eye acupuncture in promoting microvessel generation of focal cerebral ischemia-reperfusion rats by observing its effects on expressions of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR). Methods Improved suture-occluded method was adopted to establish focal cerebral ischemia-reperfusion model, and eye acupuncture was applied. Immunohistochemistry was employed to detect the microvessel density and expressions of VEGF and VEGFR2 in the brain after 3, 24 and 72h reperfusion. RT-PCR method was used to detect the expressions of VEGF mRNA and VEGFR2 mRNA. Results Compared with model group, the microvessel density was significantly increased 24h and 72h after ischemia/reperfusion in the eye acupuncture group, the expressions of VEGF and VEGFR2 at each point were also markedly increased (P＜0.05)， and the expressions of VEGF mRNA and VEGFR2 mRNA at each point were conspicuously regulated up (all P＜0.05). Conclusion Eye acupuncture can promote the microvessel generation of cerebral ischemia-reperfusion rats, and the mechanism is related to up-regulated expressions of VEGF/VEGFR.

Key words: Angiogenesis; Eye acupuncture;Vascular endothelial growth factor; Vascular endothelial growth factor receptor

中图分类号:

R743.9

宓丹1，2，马贤德3，王建4. 眼针对局灶性脑缺血再灌注大鼠VEGF/VEGFR系统表达的影响[J]. 山东大学学报(医学版), 2013, 51(2): 22-.

MI Dan1,2, MA Xian-de3, WANG Jian4 . Effects of eye acupuncture on VEGF and VEGFR expressions in focal cerebral ischemia-reperfusion rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(2): 22-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	王雪1,2,杨昆3,韩涛1,李文娜1,位坤坤1,刘奇迹2,刘学伍1. 杂合子导致CADASIL病患者家系NOTCH3基因突变[J]. 山东大学学报(医学版), 2012, 50(9): 63-.
[2]	张英丽1，吉中国1，张英瑞2. 脑白质疏松症患者认知功能水平与脑血流动力学的关系[J]. 山东大学学报(医学版), 2012, 50(2): 57-.
[3]	纪孝伟1,2，毕建忠1. 卒中后抑郁大鼠脑内FGF-2表达的研究[J]. 山东大学学报(医学版), 2011, 49(9): 30-.