替莫唑胺缓释系统的构建及体外抗胶质瘤细胞的实验研究

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (9): 6-10.

替莫唑胺缓释系统的构建及体外抗胶质瘤细胞的实验研究

姚军1,2，齐宏旭3，范晓勇4，倪石磊1,2，王建刚1,2，李新刚1，2

1.山东大学齐鲁医院神经外科，济南 250012； 2.山东大学脑科学研究所，济南 250012；
3.清华大学化工系高分子研究所，北京100084； 4.山东大学附属千佛山医院神经外科，济南 250014

收稿日期:2012-03-16 出版日期:2012-09-10 发布日期:2012-09-10
通讯作者: 王建刚(1964- )，男，教授，硕士生导师，主要从事颅脑及脊髓肿瘤的治疗研究。 E-mail：drwangjiangang@sina.com
作者简介:姚军(1982- ),男，硕士研究生，主要从事颅脑肿瘤的治疗研究
基金资助:
山东省自然科学基金(Q2007C08)；中国博士后科学基金(20100471526)；山东大学自主创新基金(2012TS157)

Controlled release system delivering temozolomide for
treatment of experimental glioma in vitro

YAO Jun1，2, QI Hong-xu3, FAN Xiao-yong4, NI Shi-lei1,2, WANG Jian-gang1,2, LI Xin-gang1,2

1. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China；
2. Institute for Brain Science, Shandong University, Jinan 250012, China;
3. Institute of Advanced Materials, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
4. Department of Neurosurgery, Qianfoshan Hospital Affiliated to Shandong University， Jinan 250014， China

Received:2012-03-16 Online:2012-09-10 Published:2012-09-10

摘要/Abstract

摘要：

目的制作一种安全有效的替莫唑胺(TM)缓释系统，使药物稳定释放，减少突释现象，减少局部使用时的神经毒性。方法首先通过电纺丝方法制作TM/聚碳酸亚丙酯(PPC)纺丝膜，探论其制作条件，分析纤维直径、载药量和包封率等参数。然后将部分纺丝膜用海藻酸(ALG)包被，在体外观察两种膜片的药物释放速度及对C6胶质瘤细胞的抑制作用。结果 TM/PPC缓释系统仅在纺丝距离为15～20cm、纺丝电压为10～15kV、聚碳酸亚丙酯质量浓度为8%时可以纺出均匀平滑的纺丝膜。纺丝纤维直径约为3μm，持续释放时间为12d。通过包被ALG，能明显减少突释的发生。两种纺丝膜在体外实验中均显示出较强的持续抑制胶质瘤细胞的能力。结论通过电纺丝方法制作替莫唑胺缓释系统切实可行。通过包被海藻酸，可以减少突释现象，使药物释放曲线更为平缓。

关键词: 替莫唑胺；聚碳酸亚丙酯；电纺丝；胶质瘤；海藻酸

Abstract:

Objective To build an effective controlled release system for Temozolomide(TM) stable delivering with low initial burst and less neurotoxicity. Methods TM-loaded PPC fiber was manufactured by electrospinning. Influences of fabricating parameters on drug release behavior were investigated. The micro-morphology of TM/PPC-fibers was researched using scanning electron microscopy(SEM). Some fiber sheets were then coated with ALG. In vitro, the release speeds of two forms of samples were studied and their cytotoxicity against C6 glioma cells was assessed. Results Uniform and smooth TM/PPC-fibers could be fabricated only when the weight concentration was 8%, the distance was 15-20cm and the voltage between the nozzle and the collection instrument was 10-15kV. The fiber diameter was about 3μm. The sustained release time was 12 days.The initial bursts of drug-fiber sheets were reduced after being covered with ALG. Cell toxicity test results showed that the above two forms of TM/PPC-fibers can continuously inhibited the C6 glioma cell, and the TM/PPC-fiber sheets were strongly cytotoxic. Conclusions Electrospinning is a feasible method to fabricate TM/PPC-fibers, and ALG covering reduces the initial burst, making the TM release curve more smooth.

Key words: Temozolomide; Poly-propylene carbonate; Electrospinning; Glioma; Alginate

中图分类号:

R739.41

姚军1,2，齐宏旭3，范晓勇4，倪石磊1,2，王建刚1,2，李新刚1，2. 替莫唑胺缓释系统的构建及体外抗胶质瘤细胞的实验研究[J]. 山东大学学报(医学版), 2012, 50(9): 6-10.

YAO Jun1，2, QI Hong-xu3, FAN Xiao-yong4, NI Shi-lei1,2, WANG Jian-gang1,2, LI Xin-gang1,2. Controlled release system delivering temozolomide for
treatment of experimental glioma in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(9): 6-10.

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