山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (11): 6-10.doi: 10.6040/j.issn.1671-7554.0.2014.429
陈洪忠1, 李海珍2, 任冬梅2
CHEN Hongzhong1, LI Haizhen2, REN Dongmei2
摘要： 目的 研究木犀草素对人脐静脉内皮EA.hy926细胞核因子E2相关因子2(Nrf2)信号通路的影响,及对H2O2诱导的细胞氧化损伤的保护作用.方法 将细胞分成对照组、H2O2处理组、阳性药物(莱菔硫烷)组、不同浓度(5、10、20、40 μmol/L)木犀草素组和不同浓度木犀草素(5、10、20、40 μmol/L)+H2O2组,采用ARE荧光素酶报告基因、Western blotting和qRT-PCR确证木犀草素对Nrf2信号通路的影响;荧光标记流式细胞仪检测内源性活性氧(ROS)水平;MTT法评价木犀草素对H2O2诱导的EA.hy926细胞的保护作用.结果 木犀草素能够剂量依赖性地增强ARE荧光强度,上调Nrf2及其下游基因血红素加氧酶-1(HO-1)、抗氧化酶NAD(P)H醌氧化还原酶-1(NQO1)和谷氨酸半胱氨酸连接酶调节亚基(GCLM)的蛋白和mRNA表达,降低内源性ROS水平;采用木犀草素和H2O2协同处理细胞,可表现出保护作用.结论 木犀草素可以激活EA.hy926细胞Nrf2信号通路,抑制H2O2诱导的细胞毒性,对EA.hy926细胞具有氧化损伤保护作用.
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