淋巴滞留性脑病对大鼠脑组织超微结构及心率变异性的影响

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (4): 66-.

淋巴滞留性脑病对大鼠脑组织超微结构及心率变异性的影响

郑冬雁1，潘燕1，刘尚明2，王立言2，孟延1，徐红岩3，李红宇4,魏蜀一1

山东大学 1.医学院生物医学工程研究所； 2.医学院组织学与胚胎学教研室；
3.医学院机能学实验教学中心，济南 250012; 4.附属济南市中心医院医保办公室，济南 250013

收稿日期:2011-11-02 出版日期:2012-04-10 发布日期:2012-04-10
通讯作者: 潘燕(1962- )，硕士研究生，副教授，硕导，主要从事生物医学信号的检测与分析的研究。E-mail: panyan@sdu.edu.cn
作者简介:郑冬雁(1982- )，硕士研究生，主要从事生物医学信号的检测与分析的研究。

Influences of lymphostatic encephalopathy on the ultrastructure
and heart rate variability in rats

ZHENG Dong-yan1, PAN Yan1, LIU Shang-ming2, WANG Li-yan2,
MENG Yan1, XU Hong-yan3, LI Hongyu4, WEI Shu-yi1

1. Institutes of Biomedical Engineering, School of Medicine; 2. Institutes of Histology and Embryology, School of Medicine;
3. Center for Functional Experiment Teaching, School of Medicine, Jinan 250012, China;
4. Medical Insurance Office of Jinan Central Hospital, Shandong University, Jinan 250013, China

Received:2011-11-02 Online:2012-04-10 Published:2012-04-10

摘要/Abstract

摘要：

目的研究淋巴滞留性脑病对自主神经系统的影响。方法采用阻断颈部淋巴引流的方法将Wistar大鼠80只制作成淋巴滞留性脑病（LE）模型，并随机分为假手术组（SHAM组）和淋巴滞留性脑病组（LE组）各40只，每组又分为术后1、7、14、21d 4个子组。采集肢体Ⅱ导联心电信号，进行心率变异性（HRV）分析，观察延髓头端腹外侧（RVLM）区脑组织形态学变化，用Western blot方法检测RVLM区eNOS蛋白的表达。结果与SHAM组相比，LE组心率变异性增大、心率减慢（P<0.05），迷走神经调节增强,RVLM区出现神经细胞变性坏死，毛细血管通透性增加，脑组织水肿，超微结构可见神经细胞凋亡、髓鞘破裂,eNOS蛋白表达下调。结论 LE造成大鼠脑组织结构与自主神经调节功能损伤，损伤的机制可能部分与下调eNOS蛋白表达有关。

关键词: 淋巴滞留性脑病；心率；一氧化氮合酶；延髓头端腹外侧区

Abstract:

Objective To investigate the influences of lymphostatic encephalopathy(LE) on the autonomic nervous system. Methods The model of LE was established by occluding lymphatic vessels and removing both shallow and deep cervical lymph nodes in Wistar rats. The rats were randomly divided into the shamoperation(SHAM) group and the LE group, 40 rats in each group. The electrocardiogram (ECG) (lead II) of rats was synchronously recorded by the PowerLab system on postoperative days 1, 7, 14 and 21. Heart rate variability (HRV) was analyzed with ECG signals. Brain morphology of the rostral ventrolateral medullas (RVLM) was observed by a light microscope and an electron microscope. eNOS protein expression in RVLM was determined by Western blot. Results Compared with the SHAM group, HRV was significantly increased, while HR was reduced（P<0.05）. The vagus nerve modulation was enhanced, necrosis of neurons appeared in RVLM, permeability of capillaries was increased, and brain tissue edema occurred. Ultrastructural observation showed neuronal apoptosis and myelin sheath disintegration. Protein expression of eNOS was significantly decreased. Conclusion LE induces injury to the cerebral tissue and autonomic nervous regulation, and the mechanism may be related to down-regulation of eNOS expression.

Key words: Lymphostatic encephalopathy; Heart rate; Nitric-oxide synthase; Rostral ventrolateral medulls

中图分类号:

R364.1+8

郑冬雁1，潘燕1，刘尚明2，王立言2，孟延1，徐红岩3，李红宇4,魏蜀一1. 淋巴滞留性脑病对大鼠脑组织超微结构及心率变异性的影响[J]. 山东大学学报(医学版), 2012, 50(4): 66-.

ZHENG Dong-yan1, PAN Yan1, LIU Shang-ming2, WANG Li-yan2, MENG Yan1, XU Hong-yan3, LI Hongyu4, WEI Shu-yi1. Influences of lymphostatic encephalopathy on the ultrastructure
and heart rate variability in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(4): 66-.

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