关键词: 脑梗死；血管性血友病因子裂解蛋白酶；血管性血友病因子抗原；凝血酶敏感蛋白1；C1423T多态性

Abstract:

Objective   To investigate the roles and significance of von Willebrand factor cleaving protease(ADAMTS13), thrombospondin 1(TSP1) and C1423T polymorphism in aged patients with acute ischemic stroke(AIS)． Methods   62 AIS and 56 age-matched healthy controls were selected. The activity of ADAMTS13 was evaluated by Residue Collagen Binding Assay(R-CBA) and the levels of TSP1 and vWF： Ag were measured by Enzyme-linked Immunosorbent Assay(ELISA) kits, while the C1423T polymorphism was determined by Polymerase Chain Reaction(PCR) and restriction enzyme to cut the amplificated product.  Results   The levels of plasma vWF: Ag and TSP1 in AIS patients were significantly higher than those of normal controls(P<0.05), while the activity of plasma ADAMTS13 in AIS patients was lower than that of normal controls(P<0.05). There were 3 patients with the C1423T polymorphism in 62 AIS patients(4.84%),which was higher than normal controls(0%).  Conclusion   Low ADAMTS13 activity may participate in the progress of acute ischemic stroke(AIS), and the level of plasma TSP1 in some degree may accelerate the crackdown of vWF by ADAMTS13. The C1423T polymorphism of the ADAMTS13 gene in the Chinese Han population may reduce the ADAMTS13 activity to participate in the occurrence of cerebral infarction.

Key words: AIS； ADAMTS13； vWF： Ag； TSP1； C1423T polymorphism