阿司匹林对小鼠子痫前期防治效果的研究

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (11): 53-.

阿司匹林对小鼠子痫前期防治效果的研究

陈丽君1，尚慧5，罗霞1，王谦2，张建平3，王立祥4

山东大学 1．齐鲁医院妇产科，济南 250012；2．齐鲁医院检验科，济南 250012；
3．齐鲁医院病理科，济南 250012；4．医学院药理研究所，济南 250012；
5．济南市中心医院妇产科，济南250013

收稿日期:2011-04-05 出版日期:2011-11-10 发布日期:2011-11-10
通讯作者: 罗霞（1972- ），副教授，博士研究生，主要从事高危妊娠和优生优育的研究。E-mail:luoxia0311@163.com
作者简介:陈丽君（1964- ），副教授，博士研究生，主要从事高危妊娠和优生优育的研究。
基金资助:
山东省优秀中青年科学家科研奖励基金资助项目（2006BS03003）

Effect of aspirin on the pre-eclamptic mouse model

CHEN Li-jun1, SHANG Hui5, LUO Xia1, WANG Qian2, ZHANG Jian-ping3, WANG Li-xiang4

1. Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan 250012, China;
2. Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China;
3. Department of Pathology, Qilu Hospital of Shandong University, Jinan 250012, China;
4.Institute of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China;
5. Department of Obstetrics and Gynecology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China

Received:2011-04-05 Online:2011-11-10 Published:2011-11-10

摘要/Abstract

摘要：

目的研究小剂量阿司匹林防治子痫前期小鼠模型的效果，探讨阿司匹林用于预防子痫前期的最低剂量和最佳时间。方法应用磷脂酰丝氨酸/磷脂酰胆碱微团，于妊娠第5～16天连续尾静脉注射法诱导建立子痫前期小鼠模型，并以应用生理盐水的正常小鼠作对照，分别于妊娠第5～16天（M0、M1、M2、M3组小鼠）、第10～16天（m0、m1、m2、m3组小鼠）：给予生理盐水（M0、m0组），阿司匹林0.13mg/d（M1、 m1组）、0.27mg/d （M2、 m2组）和0.53mg/d（M3、 m3组）。测定小鼠的血压、24h尿蛋白及胎盘体质量、胎仔体质量、胎仔数和死胎率，应用免疫组化方法观察胎盘纤维蛋白沉积面积。结果 ①M0、m0组小鼠血压、尿蛋白、胎盘纤维蛋白的沉积面积较各自正常对照组明显增加（P<0.05）；②M2、M3组各观察指标较M0差异有统计学意义（P<0.05）；M1组与M0组比较差异无统计学意义（P>0.05）；M2、M3组比较差异无统计学意义（P>0.05）；③m0、m1、m2、m3组间比较差异无统计学意义（P>0.05）。结论自妊娠第5天应用小剂量阿司匹林0.27mg/d (相当于人100mg/d)能有效改善胎盘的血液循环，减少胎盘微血栓的形成，有效改善母鼠及胎仔的预后。

关键词: 子痫前期；阿司匹林；疾病模型;小鼠

Abstract:

Objective To study the effect of low-dose aspirin on the pre-eclamptic mouse model, and explore the lowest dose and best time for using aspirin to prevent preeclampsia. Methods To establish the pre-eclamptic mouse model, mice were injected with phosphatidylserine/phosphatidylcholine microvesicles in tail veins from days 5 to 16 of pregnancy. From days 5 to 16 (groups M0, M1,M2 and M3)or days 10 to 16(groups m0, m1, m2 and m3), mice were given saline (M0 and m0), or aspirin at 0.13mg/d (M1 and m1), 0.27mg/d (M2 and m2) and 0.53mg/d (M3 and m3). Systolic blood pressure (SBP) and 24h proteinuria were measured. The weights of the placenta and fetus, and the number of fetuses were evaluated. The placental fibrin deposition area was detected using immunohistochemistry. Results ①Compared with the controls, the mice given saline(M0 or m0) showed a significant elevation in SBP, a decrease in proteinuria and a more diffuse fibrin deposition area(P<0.05). ②In all above markers, M2 and M3 had significant differences compared with M0(P<0.05), while there was no significant difference between M2 and M3(P>0.05). ③There was no significant difference among the mice given aspirin from days 10 to 16 of pregnancy (m1, m2 and m3) (P>0.05). Conclusion Using aspirin at 0.27mg/d(about 100mg/d for human) from day 5 of preg-nancy can improve placental blood circulation in the pre-eclamptic mouse model, decrease the formation of microthrombus, and benefit the mother and fetus.

Key words: Preeclampsia; Aspirin; Disease model; Mice

中图分类号:

R714.24

陈丽君1，尚慧5，罗霞1，王谦2，张建平3，王立祥4. 阿司匹林对小鼠子痫前期防治效果的研究[J]. 山东大学学报(医学版), 2011, 49(11): 53-.

CHEN Li-jun1, SHANG Hui5, LUO Xia1, WANG Qian2, ZHANG Jian-ping3, WANG Li-xiang4 . Effect of aspirin on the pre-eclamptic mouse model[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(11): 53-.

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