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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 20-25.doi: 10.6040/j.issn.1671-7554.0.2014.203

• 基础医学 • 上一篇    下一篇

樱桃汁和草莓汁激活Nrf2信号通路对砷诱导的人支气管上皮细胞损伤的保护作用

孔得刚1, 王莉莉2, 周洪雷1, 任冬梅3, 娄红祥3, 沈涛3   

  1. 1. 山东中医药大学药学院, 山东 济南 250355;
    2. 山东省日照市人民医院儿一科, 山东 日照 276800;
    3. 山东大学药学院 天然产物化学生物学教育部重点实验室, 山东 济南 250012
  • 收稿日期:2014-04-08 修回日期:2014-09-05 出版日期:2014-09-10 发布日期:2014-09-10
  • 基金资助:
    国家自然科学基金(31470419)；中国博士后基金(201104602)；山东省博士后创新基金(201002018)

Juices of cherry and strawberry confer protection of human bronchial epithelial cells against arsenic-induced toxicity via activation of Nrf2 signaling pathway

KONG Degang1, WANG Lili2, ZHOU Honglei1, REN Dongmei3, LOU Hongxiang3, SHEN Tao3   

  1. 1. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China;
    2. Division 1, Department of Paediatrics, Peopel's Hospital of Rizhao, Rizhao 276800, Shandong, China;
    3. Key Lab of Chemical Biology Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-04-08 Revised:2014-09-05 Online:2014-09-10 Published:2014-09-10
  • Contact: 沈涛。E-mail:shentao@sdu.edu.cn E-mail:shentao@sdu.edu.cn

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摘要： 目的 研究草莓汁和樱桃汁对NF-E2相关因子2（Nrf2）信号通路的影响，以及两种果汁对砷诱导的人支气管上皮（HBE）细胞损伤的保护作用。方法 选择人乳腺癌MDA-MB-231细胞系，分为空白对照组、萝卜硫素组，以及樱桃（草莓）汁1：10、1：20、1：40和1：80组（果汁稀释比例分别为1：10、1：20、1：40和1：80），采用抗氧化反应原件（ARE）荧光素酶报告基因和Westerm blotting筛选并确证两种果汁对Nrf2信号通路的影响；选择HBE细胞，分为空白对照组、萝卜硫素组，以及樱桃（草莓）汁1：10、1：20、1：40组（果汁稀释比例分别为1：10、1：20和1：40），采用QuantiChrom谷胱甘肽（GSH）试剂盒测定两种果汁对内源性抗氧化剂GSH水平的影响；采用MTT法和细胞转染技术评价两种果汁对砷诱导的HBE细胞的保护作用。结果 草莓汁和樱桃汁能够剂量依赖地增强ARE荧光强度，上调MDA-MB-231细胞中Nrf2及其下游基因NADPH苯醌氧化还原醇（NQO1）和γ-谷氨酰半胱氨酸合成酶（γGCS）蛋白的表达；两种果汁能够提高HBE细胞内源性抗氧化剂GSH水平；两种果汁和砷处理HBE细胞后，果汁预处理的细胞生存率高于砷单独处理的细胞生存率，两种果汁表现出对砷诱导细胞毒性的保护作用，而这种保护作用在转染Nrf2-siRNA后消失。结论 草莓汁和樱桃汁通过激活Nrf2信号通路抑制砷诱导的细胞毒性，对HBE细胞具有保护作用。

关键词: Nrf2信号通路, 樱桃, 草莓, 人支气管上皮细胞, 砷

Abstract: Objective To investigate the effects of cherry and strawberry juices on NF-E2-related factor 2(Nrf2) signaling pathway, and to evaluate their protection effects on arsenic-induced toxicity in human bronchial epithelial (HBE) cells. Methods Human breast cancer 231 cells were randomly divided into the control group, sulforaphen treated group, and juice treated groups (juice dilution ratios were 1:10,1:20,1:40 and 1:80, respectively), and then antioxidant response element(ARE) luciferase reporter gene assay and Western blotting analysis were adopted for screening and identification of juice on Nrf2 signaling pathway; HBE cells were randomly divided into the control group, sul-foraphen treated group, and juice treated groups (juice dilution ratios were 1:10,1:20, and 1:40, respectively), and then the QuantiChrom glutathione (GSH) assay kit were used to detect the intracellular GSH levels; MTT assay and cell transfection were carried out to evaluate their protection effects against arsenic-induced toxicity in HBE cells. Results Juices of cherry and strawberry increased the luciferase activity in a dose-dependent manner, enhanced the protein expression of Nrf2 and its downstream genes NADPH:quinone oxidoreduefase Ⅰ(NQO1) and gamma glutamyl cysteine synthetase (γGCS) in MDA-MB-231 cells, and up-regulated the intracellular GSH levels in HBE cells. Co-treatment with juice and arsenic evidently improved HBE cell survival in response to arsenic treatment alone, while the juices no longer protected the HBE cells whose Nrf2 expression was koncked down by Nrf2-siRNA. Conclusion Juices of cherry and strawberry confer the protection effects on HBE cells against arsenic-induced toxicity in a Nrf2-dependent manner.

Key words: Nrf2 signaling pathway, Cherry, Strawberry, Arsenic, Human bronchial epithelial cells

中图分类号: 

  • R966
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