山东省食品中单核细胞增生李斯特菌脉冲场凝胶电泳分型

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (8): 153-.

山东省食品中单核细胞增生李斯特菌脉冲场凝胶电泳分型

贾静1，毕振旺2，陈玉贞2，侯配斌2，张明2，邵坤2，毕振强1，2

1.山东大学公共卫生学院流行病与卫生统计学研究所，济南 250012；
2.山东省疾病预防控制中心，山东省传染病预防控制重点实验室，山东大学预防医学研究院，济南 250014

收稿日期:2011-04-11 出版日期:2011-08-10 发布日期:2011-08-10
通讯作者: 毕振强(1963- )，男，主任医师，主要从事传染病流行病学研究。E-mail：bzq63@163.com
作者简介:贾静(1986- )，女，硕士研究生，主要从事传染病流行病学研究。 E-mail：sdjiajing-1986@163.com
基金资助:
国家重大科技专项课题资助项目(2008ZX10004-008)。

Molecular typing of Listeria monocytogenes isolated from foods in Shandong Province by PulsedField Electrophoresis

JIA Jing1, BI Zhenwang2, CHEN Yuzhen2, HOU Peibin2, ZHANG Ming2, SHAO Kun2, BI Zhenqiang1，2

1. Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China;
2. Shandong Center for Disease Control and Prevention; Shandong Key Laboratory of Infectious Diseases Prevention and
Control; Institute of Preventive Medicine, Shandong University, Jinan 250014, China

Received:2011-04-11 Online:2011-08-10 Published:2011-08-10

摘要/Abstract

摘要：

目的了解山东省2007～2009年不同食品来源的单核细胞增生李斯特菌(LM)各菌株之间的遗传相关性和流行病学意义，建立分子分型数据库。方法采用PulseNet标准方法对分离到的100株单核细胞增生李斯特菌进行脉冲场凝胶电泳(PFGE)，不同菌株产生的电泳图谱经BioNumerics软件进行比对，分析菌株间的相关性。结果单核细胞增生李斯特菌DNA用AscⅠ酶切后，经PFGE得到10～15个条带，可将100株菌分为31个PFGE型别，其中以4型为主。结论山东省食品中的LM来源于不同的克隆株，但部分LM有不同程度的相关性；PFGE是分析LM同源性的有效方法，对LM的流行趋势、分布特点和分子流行病学研究具有重要的意义。

关键词: 单核细胞增生李斯特菌；脉冲场凝胶电泳；分子分型

Abstract:

Objective To investigate the genetic correlation and epidemiological significance of different genotypes of Listeria monocytogenes (LM) isolated from foods in Shandong Province during 2007-2009. Methods 100 strains were genotyped by pulsedfield gel electrophoresis (PFGE), and the images were processed by cluster analysis using computer BioNumerics software. Results Digestion of DNA with AscⅠ yielded 10 to 15 bands. The 100 Shandong Listeria monocytogenes were subtyped into 31 PFGE types, of which the type 4 was the majority. Conclusions In Shandong Province, LM strains were from different clones, but a number of LM strains were closely related; PFGE is a useful method in analyzing homology of LM, and it is of great significance in molecular epidemiological studies of LM.

Key words: Listeria monocytogenes; Pulsed-field gel electrophoresis; Molecular typing

中图分类号:

TS201.3

贾静1，毕振旺2，陈玉贞2，侯配斌2，张明2，邵坤2，毕振强1，2. 山东省食品中单核细胞增生李斯特菌脉冲场凝胶电泳分型[J]. 山东大学学报(医学版), 2011, 49(8): 153-.

JIA Jing1, BI Zhenwang2, CHEN Yuzhen2, HOU Peibin2, ZHANG Ming2, SHAO Kun2, BI Zhenqiang1，2. Molecular typing of Listeria monocytogenes isolated from foods in Shandong Province by PulsedField Electrophoresis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 153-.

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