关键词: DSCR1基因; 喉咽鳞状细胞癌;血管新生；免疫组织化学

Abstract:

Objective      To observe expression of the down syndrome candidate region 1(DSCR1) gene and its relationship with microvascular density (MVD) in laryngopharynx cancer and pericancerous tissues, and to explore the influence of the DSCR1 gene on angiogenesis in laryngopharynx cancer. Methods      94 cases of laryngopharynx squamous cell carcinoma were collected, and peri-cancerous tissues were investigated in 78 cases of them. The immunohistochemical P-V9000 method was used to determine expression of the DSCR1 gene with the rabbit DSCR1 antibody DCT3,  and vascular endothelial cells were stained to calculate MVD values with the CD34 antibody. Relationship between expression of the DSCR1 gene and MVD was statistically analyzed. Results       Positive expression rates of the DSCR1 gene were 94.9% in tumor tissues, and 35.9% in peri-cancerous tissues, and the difference was statistically significant(t=23.69, P<0.001). MVD was (17.57±10.61)bars/400 time view in laryngopharynx cancer tissues, and  (12.61±7.67) bars / 400 time view in pericancerous tissues (P＜0.05). In laryngopharynx squamous cell carcinoma tissues,  they was a positive correlation between expression of the DSCR1 gene and MVD (r=0.698, P<0.001). Conclusion      Expression of the DSCR1 gene in laryngopharynx squamous cell carcinoma is significantly increased, and it is closely related to angiogenesis in laryngopharynx cancer. DSCR1 gene can be used as a new target in gene therapy.

Key words: Down syndrome candidate region 1 gene; Laryngopharynx squamous cell carcinoma； Angiogenesis； Immunohistochemistry