关键词: 急性髓系白血病；程序性细胞凋亡因子4；转化生长因子β1

Abstract:

Objective     To investigate expressions and relationship of PDCD4 and TGF-β1 in the bone marrow of patients with acute myeloid leukemia(AML). Methods    RT-PCR, ELISA and Western blotting were used to detect  mRNA and protein expression of PDCD4 and TGF-β1 in 30 newly diagnosed AML patients, 30 AML patients who had reached complete remission (AML-CR) and 20 patients with iron deficiency anemia(IDA)as controls.   The correlation between PDCD4 and TGF-β1 was further analyzed.  Results     The results of RT-PCR indicated that mRNA expression of PDCD4 and TGF-β1 in the newly diagnosed  AML group was significantly lower than the control group(P<0.05). Though the AML-CR group was higher than the AML group, the difference was not significant(P>0.05).The results of ELISA showed that the level of TGFβ1 in  the newly diagnosed AML group was significantly lower than the other two groups(P<0.05). The results of Western blotting indicated that the expression of PDCD4 protein in the AML group was significantly lower than the control group and the AML-CR group (P<0.05). PDCD4 and TGF-β1 expression showed a positive correlation(P<0.05). Conclusion    PDCD4 and TGF-β may play important roles in the occurrence and development of AML， and might have a synergistic effect.

Key words: Acute myeloid leukemia; Programmed cell death 4; Transforming growth factor β₁