关键词: 壳聚糖；β-甘油磷酸钠；引导组织再生膜；温敏凝胶；细胞生物相容性, 主成分-线性判别；中药功效主治；药性；主成分分析；线性判别分析

Abstract:

Objective     To observe the film-forming property and evaluate the cellular biocompability of chitosan/β-Glycerophosphate salt (CS/β-GP) thermosensitive hydrogel membrane for guided tissue regeneration(GTR). Methods     A new composite membrane was synthesized by molecular self-assembly technique using the thermal phase inversion property of the CS/β-GP system. The chemical structure of the membrane was analyzed by infrared spectrometer and the tensile strength was measured by a universal testing machine. The proliferation of mouse fibroblasts (L929 cells) in vitro was preliminarily evaluated by MTT assay, which was categorized into 3 groups: experimental group A(2%CS+0.5gβ-GP), experimental group B(2%CS+1.0gβ-GP) and the control group. Results    Infrared spectrum analysis suggested that a new composite was formed through the chemical bonds between CS and β-GP molecules. The tensile strength of the membrane slightly decreased with an increase of βGP. MTT assay showed that the difference between the A value of the experimental group and that of the control group on day 3,4 and 5 when cultured with L929 cells was not significant(P＞0.05). Conclusion    The CS/β-GP composite demonstrates a film-forming property and good cellular biocompability which should be a promising biomaterial for GTR membrane fabrication.

Key words: Chitosan; β-Glycerophosphate salt; Guided tissue regeneration membrane; Thermosensitive hydrogel; Cellular biocompatibility