绞股蓝多糖含药血清对四氯化碳肝细胞损伤的保护作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (6): 46-.

绞股蓝多糖含药血清对四氯化碳肝细胞损伤的保护作用

龚秀1，吴海建1，王玉卓1，谢珊珊1，杨倚天1，李江冰1，李霞1，宋淑亮2，冯玉新1，辛华1

山东大学 1.医学院，济南 250012; 2.威海分校国际生物技术研发中心, 山东威海 264209

收稿日期:2010-12-13 出版日期:2011-06-10 发布日期:2011-06-10
通讯作者: 辛华(1950- ) ,女,教授,主要从事肝损伤和保护机制的研究。E-mail: xinhua@sdu.edu.cn
作者简介:龚秀(1987- ) ,女,本科,主要从事肝病的研究。
基金资助:
2008年度山东大学大学生创新性实验计划资助项目。

Protective effect of PGP-containing mouse serum on HepG2 cells injured by CCl4 and its possible mechanism

GONG Xiu1, WU Hai-jian1, WANG Yu-zhuo1, XIE Shan-shan1, YANG Yi-tian1, LI Jiang-bing1, LI Xia1, SONG Shu-liang2, FENG Yu-xin1, XIN Hua1

1. School of Medicine, Shandong University, Jinan 250012, China;
2. International Biotechnology Research and Development Center, Shandong University at Weihai,
Weihai 264209, Shandong, China

Received:2010-12-13 Online:2011-06-10 Published:2011-06-10

摘要/Abstract

摘要：

目的研究绞股蓝多糖(gynostemma pentaphyllum makino polysaccharide，PGP)含药血清对四氯化碳(carbon tetrachloride,CCl4)诱导的肝细胞损伤保护作用。方法采用中药血清学方法收集PGP含药血清，用CCl4诱导肝HepG2细胞损伤，设正常对照组、CCl4损伤组和不同浓度PGP含药血清保护组；细胞形态学观察细胞凋亡，MTT法检测细胞活力，生化法检测细胞培养上清液中丙氨酸氨基转移酶(alanine aminotransferase, ALT/GPT)、天门冬氨酸氨基转移酶(aspartate aminotransferase, AST/GOT)活性及western blot凝胶电泳检测细胞色素P450 2E1的表达。结果各PGP含药血清保护组肝细胞损伤程度明显减轻；细胞存活率显著升高(P<0.05)；上清液中ALT、AST活性显著降低(P<0.05)；CYP 2E1表达量显著增加。以4~8mg·kg-1·d-1保护组效果最佳。结论 PGP含药血清对CCl4诱导的HepG2细胞损伤有明显保护作用，以4~8mg·kg-1·d-1含药血清作用最显著。

关键词: 绞股蓝多糖；含药血清；肝HepG2细胞；四氯化碳；细胞凋亡

Abstract:

Objective To investigate the protective effect of gynostemma pentaphyllum makino polysaccharide(PGP)containing mouse serum on HepG2 cells injured by a 70% carbon tetrachloride(CCl4) saturated medium. Methods PGP-containing mouse serum was collected from PGP-treated mice. HepG2 cells used in the experiment were divided into the following groups: the control group, the CCl4 injury group and the PGP-containing mouse serum protection groups with different concentrations of PGP. A 70% CCl4 saturated medium was used to induce HepG2 cell injury in all groups except the control group. Cell morphological change was observed under a brightfield microscope, cell viability by 4-methyl- tetrazolium (MTT) chromatometry, the degree of cell damage by measuring supernatant alanine aminotransferase (GPT) and aspartate aminotransferase (GOT) activities, and expression level of the cytochrome P450 2E1 (CYP2E1) protein by Western blot. ResultsCompared with the CCl4 injury group, the PGP-containing serum protection groups had significant morphological improvement, and their survival rate was significantly higher(P<0.05). ALT and AST activities in the supernatant were significantly decreased (P<0.05) while expression level of CYP2E1 was increased.The protective effect was the most obvious in the 48mg·kg-1·d-1 PGP-containing serum protection group. Conclusion Pre-treatment of PGP-containing mouse serum is shown to possess a significant protective effect on HepG2 cells injured by CCl4 especially at 4-8mg·kg-1·d-1.

Key words: Gynostemma pentaphyllum makino; Drug-containing serum; HepG2 cells; Carbon tetrachloride; Apoptosis

中图分类号:

R961.1

龚秀1，吴海建1，王玉卓1，谢珊珊1，杨倚天1，李江冰1，李霞1，宋淑亮2，冯玉新1，辛华1. 绞股蓝多糖含药血清对四氯化碳肝细胞损伤的保护作用[J]. 山东大学学报(医学版), 2011, 49(6): 46-.

GONG Xiu1, WU Hai-jian1, WANG Yu-zhuo1, XIE Shan-shan1, YANG Yi-tian1, LI Jiang-bing1, LI Xia1, SONG Shu-liang2, FENG Yu-xin1, XIN Hua1. Protective effect of PGP-containing mouse serum on HepG2 cells injured by CCl4 and its possible mechanism[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 46-.

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