胃癌中VEGF-A、VEGF-D和podoplanin的表达及其与淋巴道转移的研究

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (5): 61-.

胃癌中VEGF-A、VEGF-D和podoplanin的表达及其与淋巴道转移的研究

田小林，罗庆伟，蒋志庆，林静

桂林医学院附属医院胃肠外科，广西桂林 541001

收稿日期:2011-01-23 出版日期:2011-05-10 发布日期:2011-05-10
作者简介:田小林，男，副教授，副主任医师，硕士研究生导师，主要从事胃肠道肿瘤的研究。 E-mail：tianxiaolin@glmc.edu.cn
基金资助:
桂林市科学研究与技术开发计划项目（20080209）

Expressions of VEGF-A, VEGF-D and podoplanin in gastric carcinoma and their relationship with lymph node metastases

TIAN Xiao-lin, LUO Qing-wei， JIANG Zhi-qing， LIN Jing

Department of Gastric & Intstinal Surgery, Affiliated Hospital Guilin Medical College of Guilin, Guilin 541001, Guangxi, China

Received:2011-01-23 Online:2011-05-10 Published:2011-05-10

摘要/Abstract

摘要：

目的探讨胃癌患者血管内皮生长因子A和D ( vascular endothelial growth factor-A and-D,VEGF-A and VEGF-D)在胃癌组织、癌旁组织、正常胃组织中的表达及其在胃癌进展中的作用。观察淋巴管内皮细胞podoplanin的表达，为研究胃癌淋巴道转移的分子机制提供形态学依据。方法应用免疫组织化学SP法检测54例胃癌组织、癌旁组织和12例正常胃组织中VEGF-A、VEGF-D、podoplanin的表达，检测胃癌组织及癌旁组织中的微淋巴管密度（lymphatic microvessel density, LMVD）。结果胃癌癌组织、癌旁组织中VEGF-A和VEGF-D表达阳性率明显高于正常胃组织(P<0.01 ),其中在淋巴结转移组两者的表达明显高于淋巴结未转移组(P<0.05 )。VEGF-A和VEGF-D在胃癌组织中的表达呈正相关（r= 0.365， P=0.007），并与淋巴结转移有关(P<0.01)。胃癌癌组织血管内皮podoplanin不染色，淋巴管内皮细胞podoplanin阳性表达，经计数微淋巴管数量，癌组织和癌旁组织LMVD明显高于正常组织，VEGF-A、VEGF-D阳性组LMVD值分别为(9.23±2.49)、(9.29±2.18)，较阴性组(7.53±1.81)、(7.44±1.88)显著增高。结论胃癌中VEGF-A与VEGF-D高表达与胃癌淋巴结转移密切相关, podoplanin可选择性的在胃癌组织淋巴管内皮表达，能明确地区分血管及淋巴管成分，可作为一个淋巴管内皮细胞较特异的标记物。由于微淋巴管密度在VEGF-A与VEGF-D阳性表达组癌组织中的表达明显增高，表明淋巴管数量的增加可能与两者协同作用有关。

关键词: 胃癌;血管内皮生长因子A; 血管内皮生长因子D; Podoplanin；淋巴管；微淋巴管密度

Abstract:

Objective To explore expressions of vascular endothelial growth factor(VEGF) A and D in gastric cancer, pericancerous gastric tissue and normal gastric tissues and their relationship with progression of gastric carcinoma.To observe expression of podoplanin in lymphatic endothelial cells in gastric carcinoma so as to provide morphological data for the study of the molecular mechanism of tumor metastasis. Methods The immunohistochemical SP method was used to examine expressions of VEGF-A and D in 54 gastric cancer tissues, 54 peri-cancerous gastric tissues, 12 normal gastric tissues, and expression of podoplanin in gastric carcinoma and normal gastric tissue.Lymphatic microvessel density(LMVD) in gastric cancer tissue and pericancerous gastric tissues were calculated. Results Positive expression rates of VEGF-A and D in gastric cancer tissues and peri-cancerous gastric tissues were significantly higher than those in normal gastric tissues (P<0. 01), expressions of VEGF-A and D in the gastric cancer group and the lymph node metastasis group were significantly different(P<0. 05). And expressions of VEGF-A and D were of significantly positivecorrelation(r=0.365,P=0.007). Expressions of VEGF-A and D in gastric carcinoma were positively correlated with lymph node metastasis (P<0. 01 ), podoplanin was not stained in blood vessels but was positively expressed in gastric carcinoma lymphatics. LMVD of gastric carcinoma tissues and pericancerous gastric tissues was obviously higher than that in normal gastric tissues. MLVD detected from the VEGF-A, D positive group respectively(9.23±2.49)，(9.29±2.18) was significantly higher than that detected from the VEGF-A, D negative group respectively(7.53±1.81), (7.44±1.88). Conclusion High expressions of VEGF-A and VEGF-D in gastric cancer cells are closely related to lymph nodemetastasis. Podoplanin is selectively expressed in lymphatics of gastric carcinoma and could correctly distinguish blood vessels from lymphatic vessels. It could be regarded as a specific marker of lymphatic vessels. LMVD of the VEGF-A and D positive groups increases in gastric carcinoma， therefore， the increase of the number of lymphatic vessels likely contributes to their synergism.

Key words: Gastric cancer;Vascular endothelial growth factor-A; Vascular endothelial growth factor-D; Ppodoplanin; Lymphatic vessel; Lymphatic micro-vessel density

中图分类号:

R735.2

田小林，罗庆伟，蒋志庆，林静. 胃癌中VEGF-A、VEGF-D和podoplanin的表达及其与淋巴道转移的研究[J]. 山东大学学报(医学版), 2011, 49(5): 61-.

TIAN Xiao-lin, LUO Qing-wei， JIANG Zhi-qing， LIN Jing. Expressions of VEGF-A, VEGF-D and podoplanin in gastric carcinoma and their relationship with lymph node metastases[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(5): 61-.

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