心脉隆注射液对新生SD大鼠海马神经干细胞增殖分化的影响

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (6): 46-50.

心脉隆注射液对新生SD大鼠海马神经干细胞增殖分化的影响

满潇潇1，于君2，王晓云1，刘震1，罗珊珊1，谢兆宏1，赵翠萍1，毕建忠1

1.山东大学第二医院神经内科，济南 250033； 2.山东中医药大学附属医院院内感染科，济南 250011

收稿日期:2012-01-12 出版日期:2012-06-10 发布日期:2012-06-10
通讯作者: 毕建忠（1961- )，男，主任医师，博士生导师，主要从事老年性痴呆及脑血管病的研究。 E-mail:bjz@sdu.edu.cn
作者简介:满潇潇（1984- )，女，硕士研究生，主要从事老年性痴呆及脑血管病的研究
基金资助:
国家自然科学基金资助项目（81171214)；山东省优秀中青年科学家科研奖励基金资助项目（BS2009SW054)

Effect of xinmailong injection on proliferation and differentiation of
neural stem cells in the hippocampus of newborn rats

MAN Xiao-xiao1, YU Jun2, WANG Xiao-yun1, LIU Zhen1, LUO Shan-shan1,
XIE Zhao-hong1, ZHAO Cui-ping1， BI Jian-zhong1

1. Department of Neurology, The Second Hospital of Shandong University, Jinan 250033, China;
2. Department of Nosocomial Infection, Affiliated Hospital of Shandong University of
Traditional Chinese Medicine, Jinan 250011, China

Received:2012-01-12 Online:2012-06-10 Published:2012-06-10

摘要/Abstract

摘要：

目的初步探讨心脉隆注射液对新生SD大鼠海马神经干细胞增殖分化作用的影响。方法分离新生SD大鼠海马组织，采用无血清及单克隆培养方式获得大量神经干细胞，将不同浓度心脉隆注射液加入第3代神经干细胞单细胞悬液中有血清贴壁培养并分实验组和对照组，应用免疫荧光及细胞化学染色检测Nestin、5-溴脱氧尿嘧啶核苷(BrdU)、微管相关蛋白2(MAP-2)、胶质纤维酸性蛋白(GFAP)的表达，计算MAP2阳性细胞百分率。四甲基偶氮唑盐(MTT)比色法观察心脉隆注射液对神经干细胞增殖能力的影响。结果海马NSCs在分化后，各心脉隆注射液干预组MAP2阳性细胞比例均较空白对照组增高(P<0.05)，MTT结果显示培养5d后各药物组NSCs增殖能力均较对照组增强(P<0.05)。结论心脉隆注射液具有促进神经干细胞增殖并向神经元方向分化的作用。

关键词: 核苷类；干细胞；细胞分化；海马

Abstract:

Objective To investigate the effects of xinmailong injection on the proliferation and differentiation of neural stem cells from the hippocampus of neonatal Sprague Dawley rats. Methods The hippocampal tissues of the newborn rat were dissociated and cultured with serum-free medium using single-cell cloning technique. Hippocampal neural stem cells of the 3rd passage were induced by xinmailong injection with various concentrations of serum and divided into the various experimental groups and the control group. Nestin, 5-bromo-2-deoxyuricine(BrdU), microtubule-associated protein 2(MAP-2), and glial fibrillary acidicprotein (GFAP) expression were detected using immunofluorescence cytochemical staining. The percentages of MAP-2 positive cells were obtained. Monotetrazolium (MTT) assay was used to detect neural stem cell (NSCs) proliferation under the condition of xinmailong injection. Results The percentages of MAP-2 positive cells in the various experimental groups were higher(P<0.05) than the control group. After 5 days cultivation, the NSCs growth tendency in the experimental groups was increased compared to the control group. Conclusion Xinmailong injection may facilitate the proliferation of NSCs and promote the differentiation of NSCs into neurons.

Key words: Nucleosides; Stem cells; Cell differentiation; Hippocampus

中图分类号:

R741

满潇潇1，于君2，王晓云1，刘震1，罗珊珊1，谢兆宏1，赵翠萍1，毕建忠1. 心脉隆注射液对新生SD大鼠海马神经干细胞增殖分化的影响[J]. 山东大学学报(医学版), 2012, 50(6): 46-50.

MAN Xiao-xiao1, YU Jun2, WANG Xiao-yun1, LIU Zhen1, LUO Shan-shan1,XIE Zhao-hong1, ZHAO Cui-ping1， BI Jian-zhong1. Effect of xinmailong injection on proliferation and differentiation of
neural stem cells in the hippocampus of newborn rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 46-50.

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