姜黄素对多巴胺诱导PC12细胞凋亡的拮抗作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (2): 29-33.

姜黄素对多巴胺诱导PC12细胞凋亡的拮抗作用

王翔宇1，梅元武2，杨红卫3

1.三峡大学第三临床学院，葛洲坝中心医院神经内科，湖北宜昌 443002；
2.华中科技大学同济医学院附属协和医院神经内科，武汉 430010；
3.三峡大学医学院生理学教研室，湖北宜昌 443002

收稿日期:2010-11-11 出版日期:2011-02-10 发布日期:2011-02-10
作者简介:王翔宇(1972- )，男，副主任医师，主要从事脑血管病诊断与治疗的研究。 E-mail: wxy72315@sina.com

Protective effect of curcumin on dopamineinduced apoptosis in PC12 cells

WANG Xiang-Yu1， MEI Yuan-Wu2， YANG Hong-Wei3

1. Department of Neurology, Gezhouba Central Hospital, Third Clinical Hospital of China Three Gorges University,
Yichang 443002, Hubei, China; 2. Department of Neurology, Affiliated Union Hospital of Tongji Medical College of
Huazhong University of Science and Technology, Wuhan 430010, China;
3. Department of Physiology, China Three Gorges University, Yichang 443002, Hubei, China

Received:2010-11-11 Online:2011-02-10 Published:2011-02-10

摘要/Abstract

摘要：

目的探讨姜黄素(Cur) 对多巴胺氧化应激损伤PC12 细胞的保护作用。方法以多巴胺（DA)损伤PC12细胞为氧化应激损伤的模型,采用MTT法检测细胞增殖状况，Hoechst染色检测细胞核形态，碘化丙啶(PI) 染色流式细胞术(FCM) 检测细胞凋亡, 罗丹明123(Rh123) 染色FCM检测细胞线粒体膜电位(MMP)。结果多巴胺（50~800μmol/L，24h）呈浓度依赖性地损伤PC12细胞，姜黄素（10~40μmol/L）对PC12细胞无毒性作用，但呈浓度依赖性地减轻DA对PC12细胞的毒性作用，抑制DA诱导PC12细胞凋亡，减轻DA对PC12 MMP的降低作用。结论姜黄素可抑制DA诱导PC12细胞凋亡，对DA氧化应激损伤神经元具有保护作用，其机制可能与姜黄素抑制MMP 的降低有关。

关键词: 姜黄素；多巴胺；氧化性应激；PC12细胞；膜电位

Abstract:

Objective To explore the protective effect of curcumin (Cur) on apoptosis of PC12 cells induced by dopamine (DA) oxidative stress. Methods Using DA to induce PC12 cell damage, the model of oxidative stress-induced neuron damage was established. Proliferation of PC12 cells was observed by 3-［4 ,5dimethylthiazolyl ］-2 ,5diphenyltetrazolium bromide (MTT) assay. Morphology of the nucleus was observed by Hoechst staining. Apoptosis of PC12 cells was detected by propidium iodide(PI) staining flow cytometry (FCM). The mitochondrial membrane potential(△Ψm) was determined by Rh123 staining flow cytometry. Results ① DA damaged PC12 cells in a concentration-dependent manner after application of DA (50-800μmol/L) for 24h, and MTT assay showed that Cur （10-40μmol/L） had no toxic effect on PC12 cells . ② Cur reduced the toxic effect induced by DA on PC12 cells in a concentration-dependent manner. ③ Cur reducing the DA-induced toxic effect on PC12 cells was confirmed by cellular morphologic experiments. ④ PI staining FCM showed that Cur inhibited cellular apoptosis induced by DA and this effect was also confirmed by Hoechst staining. ⑤ The level of △Ψm was significantly decreased in PC12 cells after 100 and 200μmol/L DA treatment for 24h, which was significantly ameliorated by Cur (20 and 40μmol/L) treatment. Conclusion Cur has a protective effect on PC12 cell damage induced by DA oxidative stress and this effect may be ascribed to the amelioration of the decreasing level of △Ψm.

Key words: Curcumin; Dopamine; Oxidative stress; PC12 cells; Membrane potentials

中图分类号:

R338.2

王翔宇1，梅元武2，杨红卫3. 姜黄素对多巴胺诱导PC12细胞凋亡的拮抗作用[J]. 山东大学学报(医学版), 2011, 49(2): 29-33.

WANG Xiang-Yu1，MEI Yuan-Wu2，YANG Hong-Wei3. Protective effect of curcumin on dopamineinduced apoptosis in PC12 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 29-33.

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