饮食中晚期糖基化终产物对糖尿病肾病大鼠肾脏的损害作用

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山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (1): 6-.

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饮食中晚期糖基化终产物对糖尿病肾病大鼠肾脏的损害作用

毕铭霞1，2 ，朱彬2，张瑞斌2，逄曙光3

山东大学 1.医学院，济南 250012； 2.附属济南市中心医院血液净化中心，济南 250013；
3.附属济南市中心医院内分泌科，济南 250013

收稿日期:2010-08-23 出版日期:2011-01-10 发布日期:2011-01-10
通讯作者: 逄曙光(1966- )，女，教授，硕士生导师，主要从事内分泌性疾病、肾脏病与代谢性疾病的研究。
作者简介:毕铭霞(1983- )，女，硕士研究生，主要从事糖尿病肾病与终末期肾病的研究。

Damage to the kidney of rats with diabetic nephropathy by high advanced glycation end products in the diet

BI Ming-xia1,2, ZHU Bin2, ZHANG Rui-bin2, PANG Shu-guang3

1. School of Medicine, Shandong University, Jinan 250012, China;
2. Blood Purification Center, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
3. Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China

Received:2010-08-23 Online:2011-01-10 Published:2011-01-10

摘要/Abstract

摘要：

目的观察饮食中晚期糖基化终产物（AGEs）对糖尿病肾病（DN）大鼠肾脏的影响，探讨其作用机制。方法 SD大鼠40只，随机选取10只为空白对照组， 30只大鼠DN模型建立成功，随机分为3组：高AGEs饲料组、低AGEs饲料组及普通饲料组，每组10只。分别于第9周及第13周对各组大鼠进行检测：考马斯亮蓝法测定24h尿蛋白量，速率法测定血肌酐(Scr)和尿肌酐，硫代巴比妥酸反应法（TBARS）测定24h尿及肾组织匀浆中丙二醛(MDA)含量，ELISA法测定血清及肾组织匀浆中羧甲基赖氨酸（CML）含量，比色法测定血清及肾组织匀浆中硒谷胱甘肽过氧化物酶(SeGSHPx)活性，计算内生肌酐清除率(Ccr)。结果 3组DN大鼠各指标与空白对照组相比，差异有统计学意义（P＜0.05）。与普通饲料组相比，高AGEs饲料组大鼠24h尿蛋白、24h尿及肾组织匀浆中MDA含量、血清及肾组织匀浆中CML含量显著增加（P＜0.05），血清及肾组织匀浆中SeGSHPx活性显著降低（P＜0.05）；低AGEs饲料组大鼠24h尿蛋白、24h尿及肾组织匀浆中MDA含量、血清及肾组织匀浆中CML含量显著减少（P＜0.05），血清及肾组织匀浆中SeGSHPx活性显著升高（P＜0.05）。3组DN大鼠之间Scr及Ccr差异无统计学意义（P＞0.05）。结论高AGEs饲料促进DN大鼠肾功能恶化，其作用机制与抗氧化体系受损、氧化应激增强有关。

关键词: 晚期糖基化终产物；糖尿病肾病；氧化应激；丙二醛；硒谷胱甘肽过氧化物酶

Abstract:

Objective To study the effect of oral advanced glycation end products(AGEs) on rats with diabetic nephropathy(DN) and its mechanism. Methods 10 rats were randomly selected from 40 male Sprague-Dawley rats as the control group. The other 30 rats which developed DN were randomly divided into three groups: the AGEs-rich diet group, the AGEs-poor diet group and the common diet group, 10 rats in each group. Each group was studied at the 9th and 13th weeks. The following parameters were detected with different methods: 24 h urinary albumin with the Coomassie brilliant blue method, serum creatinine and urine creatinine by an automatic biochemistry analyzer, content of MDA in 24 h urine and nephridial tissue homogenate by TBARS, content of CML in serum and nephridial tissue homogenate by ELISA, and activity of SeGSHPx in serum and nephridial tissue homogenate by a spectrophotometer. Ccr was calculated. Results Significant differences were found between the three DN groups and the control group（P＜0.05）. Compared to the common diet group, the following variables in the AGEs-rich diet group were dramatically increased: the 24 h urine protein, MDA levels in 24 h urine and nephridial tissue homogenate, and CML levels in serum and nephridial tissue homogenate（P＜0.05); while SeGSHPx activities in serum and nephridial tissue homogenate decreased（P＜0.05). In contrast, the AGEs-poor diet group showed opposite results in all the above variables. No significant difference was observed in Scr and Ccr among the three DN rat groups（P＞0.05）. Conclusion An AGEs-rich diet impairs the renal function of DN rats and the mechanism is probably related to damage to anti-oxidation ability and enhancement of oxidative stress.

Key words: Advanced glycation end products; Diabetic nephropathy; Oxidative stress; Malondialdehyde; Se-glutathione peroxidase

中图分类号:

R332

毕铭霞1，2 ，朱彬2，张瑞斌2，逄曙光3. 饮食中晚期糖基化终产物对糖尿病肾病大鼠肾脏的损害作用[J]. 山东大学学报(医学版), 2011, 49(1): 6-.

BI Ming-xia1,2, ZHU Bin2, ZHANG Rui-bin2, PANG Shu-guang3. Damage to the kidney of rats with diabetic nephropathy by high advanced glycation end products in the diet[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 6-.

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