h17细胞相关因子在人脑胶质瘤中的测定

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (6): 87-91.

h17细胞相关因子在人脑胶质瘤中的测定

郑鲲鹏1，宋辉2，樊明德1，张源3，李科1，王成伟1

山东大学 1.第二医院神经外科，济南 250033； 2.附属济南市中心医院神经外科，
济南 250000； 3.齐鲁医院急诊科，济南 250012

收稿日期:2012-01-17 出版日期:2012-06-10 发布日期:2012-06-10
通讯作者: 王成伟(1968- )，男，教授，硕士生导师，主要从事脑胶质瘤及脑血管病的研究。 E-mail:wangchengwei@sdu.edu.cn
作者简介:郑鲲鹏(1985- )，男，硕士研究生，主要从事脑胶质瘤及脑血管病的研究

The detection of Th17 cell correlation factors expression in glioma

ZHENG Kun-peng1, SONG Hui2, FAN Ming-de1, ZHANG Yuan3, LI Ke1, WANG Cheng-wei1

1. Department of Neurosurgery, the Second Hospital of Shandong University, Jinan 250033, Chna;
2. Department of Neurosurgery, Jinan Central Hospital, Jinan 250000, Chna;
3. Department of Emergency, Qilu Hospital of Shandong University, Jinan 250012, Chna

Received:2012-01-17 Online:2012-06-10 Published:2012-06-10

摘要/Abstract

摘要：

目的了解Th17细胞相关因子在人脑胶质瘤组织中的表达，确定Th17的存在及浸润程度。方法应用免疫组化二步法检测Th17细胞相关因子CD4+和IL-17在34例脑胶质瘤和10例正常脑组织病理切片中的表达，采用实时荧光定量逆转录聚合酶链反应(QRT-PCR)技术，检测IL-17mRNA、RORγtmRNA在人脑胶质瘤组织中的表达量。结果 IL-17、CD4+在正常脑组织表达不明显，在低级别脑胶质瘤中表达较少，CD4+和IL-17在高级别脑胶质瘤中表达明显，组间比较差异有统计学意义(P<0.05，P<0.05，P<0.01)。IL-17mRNA和RORγt mRNA在正常脑组织、低级别、高级别脑胶质瘤中的表达量随恶性程度升高而增加，组间比较差异有统计学意义(P<0.05，P<0.01，P<0.01)。结论脑胶质瘤中存在Th17的浸润，其表达量随脑胶质瘤恶性程度的升高而增加。

关键词: 神经胶质瘤；Th17细胞；白细胞介素17；维A酸相关核孤儿受体-γ

Abstract:

Objective To investigate Th17 existence and infiltration in glioma by testing Th17 correlation factors expression. Methods The presence of CD4+ and IL-17 were measured by immunohistochemistry in 34 human glioma tissues and 10 normal brain tissues. Expression levels of both IL-17mRNA and RORγtmRNA were detected by QRT-PCR technology. Results Neither CD4+ nor IL-17 had positive staining in the 10 normal brain tissues. In the low grade group (WHOⅠ-Ⅱ), there were low expression levels of both CD4+ and IL-17. Compared with those of the low-grade group, there were obviously higher expression levels of both CD4+ and IL-17 in the highgrade group (WHOⅢ-Ⅳ). There was significantly statistical difference among the comparisons(P<0.05，P<0.05，P<0.01). The QRT-PCR result showed that expression levels of IL-17AmRNA and RORγtmRNA had a positive correlation with the malignancy of human glioma. There was significant statistical difference among the comparisons of the three groups(P<0.05，P<0.05，P<0.01). Conclusion There is Th17 infiltration in glioma,whose expression level has a positive correlation with the malignancy of human glioma.

Key words: Glioma; Th17cells; Interleukin-17; Retinoic acidrelated orphan receptor

中图分类号:

R739.41

郑鲲鹏1，宋辉2，樊明德1，张源3，李科1，王成伟1. h17细胞相关因子在人脑胶质瘤中的测定[J]. 山东大学学报(医学版), 2012, 50(6): 87-91.

ZHENG Kun-peng1, SONG Hui2, FAN Ming-de1, ZHANG Yuan3, LI Ke1, WANG Cheng-wei1. The detection of Th17 cell correlation factors expression in glioma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 87-91.

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