人骨髓间充质干细胞诱导成软骨细胞过程中免疫原性改变的实验研究

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (11): 24-28.

人骨髓间充质干细胞诱导成软骨细胞过程中免疫原性改变的实验研究

古彦铮1，3，蔡燕2，倪莉2，黄晨2，杨惠林2，3，张学光1，3，施勤2，3

1．苏州大学附属第一医院临床免疫中心，江苏苏州 215007；
2.苏州大学附属第一医院骨科，江苏苏州 215006；
3.江苏省干细胞重点实验室，江苏苏州 215007

收稿日期:2010-05-21 出版日期:2010-11-16 发布日期:2010-11-16
通讯作者: 施勤（1971- ），女，副教授，博士，主要从事干细胞和组织工程的研究。
作者简介:古彦铮（1982- ），女，博士研究生，主要从事干细胞免疫调节的研究。

Alterations of immunogenicity in the differentiation from human bone marrow derived mesenchymal stem cells to chondrocytes

GU Yan-zheng1,3, CAI Yan2, NI Li2, HUANG Chen2, YANG Hui-lin2,3, ZHANG Xue-guang1,3, SHI Qin2,3

1. Center of Clinical Immunology, the First Affiliated Hospital of Soochow University, Suzhou 215007， Jiangsu, China;
2. Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006， Jiangsu, China;
3. Jiangsu Province Key Lab of Stem Cell, Suzhou 215007, Jiangsu, China

Received:2010-05-21 Online:2010-11-16 Published:2010-11-16

摘要/Abstract

摘要：

目的探讨人骨髓来源间充质干细胞（BMSCs）诱导分化成软骨细胞过程中协同刺激分子的表达及其对细胞免疫原性改变的影响。方法用密度梯度离心法分离骨髓，体外培养BMSCs，经形态学观察、流式细胞仪鉴定后诱导成软骨样细胞，用流式检测细胞表面协同刺激分子的表达情况;将未分化的BMSCs及诱导后的成软骨样细胞分别与T细胞共培养，采用3H-TdR法检测T细胞的增殖情况。结果人BMSCs不表达或低表达协同刺激分子CD28、CD80、CD83、CD86，抑制T细胞增殖；而诱导后的成软骨细胞上调表达CD28、CD80、CD83、CD86，并促进T细胞增殖。结论人BMSCs对T细胞增殖有抑制作用；而诱导后的成软骨细胞免疫原性增强，促进T细胞增殖。

关键词: 间充质干细胞；协同刺激分子；成软骨细胞；免疫原性

Abstract:

Objective To investigate expressions of co-stimulatory molecules and their effects on alterations of immunogenicity on human bone marrow derived mesenchymal stem cells and differentiated chondrocytes. Methods Human bone marrow mesenchymal stem cells (BMSCs) were isolated by density gradient centrifugation and confirmed by an inverted phase contrast microscope and flow cytometry (FCM). BMSCs were induced to differentiate into chondrocytes. Expressions of co-stimulatory molecules were quantified by FCM. T cells were incubated respectively with undifferentiated BMSCs and chondrocytes, while the 3H-TdR incorporation method was carried out to evaluate T cell proliferation. Results BMSCs did not express co-stimulatory molecules CD28, CD80, CD83 and CD86，but inhibited the proliferation of T cells. Expressions of CD28, CD80, CD83, and CD86 in hBMSCs and differentiated chondrocytes were up-regulated and proliferation of T cells was promoted. Conclusion BMSCs can inhibit proliferation of T cells in vitro by increasing immunogenicity of BMSC-differentiated chondrocytes.

Key words: Mesenchymal stem cell; Co-stimulatory molecules; Chondrocytes; Immunogenicity

中图分类号:

R394.26

古彦铮1，3，蔡燕2，倪莉2，黄晨2，杨惠林2，3，张学光1，3，施勤2，3. 人骨髓间充质干细胞诱导成软骨细胞过程中免疫原性改变的实验研究[J]. 山东大学学报(医学版), 2010, 48(11): 24-28.

GU Yan-zheng1,3, CAI Yan2, NI Li2, HUANG Chen2, YANG Hui-lin2,3, ZHANG Xue-guang1,3, SHI Qin2,3. Alterations of immunogenicity in the differentiation from human bone marrow derived mesenchymal stem cells to chondrocytes[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(11): 24-28.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed