体外培养细胞拉伸加载装置的研制

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (10): 25-28.

体外培养细胞拉伸加载装置的研制

张晓燕1, 孙玉德2, 袁晓3, 王洪玲1, 赵芳1, 刘东旭1

1. 山东大学口腔医学院，山东省口腔生物医学重点实验室，济南 250012；
2. 哈尔滨工业大学（威海）信息学院，山东威海 264209； 3. 青岛市立医院口腔科，山东青岛 266075

收稿日期:2010-06-12 出版日期:2010-10-16 发布日期:2010-10-16
通讯作者: 刘东旭（1963- ），男，博士，教授，硕士生导师，研究方向为口腔正畸矫治机制。 E-mail: liudongxu@sdu.edu.cn
作者简介:张晓燕（1984- ），女，硕士研究生，研究方向为正畸牙齿移动骨改建信号转导通路的调控机制。孙玉德(1964- ),男，硕士，教授，主要从事微电子技术研究。
基金资助:
国家自然科学基金资助项目（30770528）；山东大学口腔医学院匹配课题项目（P2009008）。

Development of stretchingloading equipment for cultured cells in vitro

ZHANG Xiao-yan1, SUN Yu-de2, YUAN Xiao3, WANG Hong-ling1, ZHAO Fang1, LIU Dong-xu1

1. School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine,
Jinan 250012, China; 2. The Information College in Harbin Institute of Technology (Weihai), Weihai 264209,
Shandong, China; 3. The Department of Stomatology in Qingdao Municipal Hospital, Qingdao 266075, Shandong, China

Received:2010-06-12 Online:2010-10-16 Published:2010-10-16

摘要/Abstract

摘要：

目的为模拟在体细胞的应力环境，研制一种对离体细胞施加周期性拉伸应变的实验装置。方法细胞拉伸加载装置由计算机控制的硬件和软件组成。硬件包括真空负压系统、控制电路和抗干扰优化装置，软件是基于C语言的系统开发套件。此装置利用单片机控制电磁阀和真空泵抽吸柔性细胞培养膜形成负压而使基底膜产生拉伸应变,使贴附生长的细胞受到牵张力的作用。通过对大鼠血管平滑肌细胞的加载实验，进一步验证装置的可行性。结果加载装置可以对加载力值、牵张及松弛时间和持续工作时间进行有效控制；加载后血管平滑肌细胞在培养板中稳定生长并有增殖活性。结论本装置满足离体细胞周期性拉伸加载的要求，并可广泛用于拉伸加载条件下的细胞力学生物学的研究。

关键词: 细胞培养；拉伸应变；加载装置

Abstract:

Objective To design cyclic stretching loading equipment for cultured cells to simulate the stress environment in vivo. Methods The stretchingloading equipment consisted of hardware and software which were controlled by computer. The hardware was composed of a vacuum system, control circuit and anti-jamming device, and the software was based on C language. The bottom of the cultured cellular layer was stretched by a MCU-controlled vacuum pump and solenoid valve, and therefore, the cells the adhered to the plate were stretched. This device was further verified through the loading on vascular smooth muscle cells. Results The loading equipment control force value, time of stretching and relaxation, and working time were effective; Vascular smooth muscle cells grew stably on the culture plate and exhibited proliferative activity under loading. Conclusion The equipment fits well for cyclic stretching-loading on cultured cells in vitro, and will be widely used for tensile mechanical loading of cell mechano-biology.

Key words: Cell culture; Stretching strain; Loading equipment

中图分类号:

R318.6

张晓燕1, 孙玉德2, 袁晓3, 王洪玲1, 赵芳1, 刘东旭1 . 体外培养细胞拉伸加载装置的研制[J]. 山东大学学报(医学版), 2010, 48(10): 25-28.

ZHANG Xiao-yan1, SUN Yu-de2, YUAN Xiao3, WANG Hong-ling1, ZHAO Fang1, LIU Dong-xu1. Development of stretchingloading equipment for cultured cells in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 25-28.

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