慢性丙型肝炎患者血清白介素-8表达水平与基因型及抗病毒治疗的相关性

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (8): 100-102.

慢性丙型肝炎患者血清白介素-8表达水平与基因型及抗病毒治疗的相关性

张立新1，2，安勇2，徐皖苏3，张孝国2，刘靓雯3，于进红2，张琴冈2

1.山东大学医学院，济南 250012； 2.济南市传染病医院肝病科，济南 250021；
3.济南市传染病医院中心实验室，济南 250021

收稿日期:2009-12-26 出版日期:2010-08-16 发布日期:2010-08-16
作者简介:张立新（1968- ），女，医学硕士，副主任医师，主要从事病毒性肝炎的研究。 E-mail：lixinzhang68@sina.com

Relationship of expression level of serum IL-8 with HCV genotypes and antivirus treatment in patients with chronic hepatitis C

ZHANG Li-xin1，2, AN Yong2, XU Wan-su3, ZHANG Xiao-guo2,LIU Jing-wen3, YU Jin-hong2, ZHANG Qin-gang2

1. School of Medicine,Shandong University, Jinan 250012, China;
2. Department of Liver Diseases, Jinan Infectious Disease Hospital , Jinan 250021, China;
3. Central Laboratory, Jinan Infectious Disease Hospital, Jinan 250021, China

Received:2009-12-26 Online:2010-08-16 Published:2010-08-16

摘要/Abstract

摘要：

目的探讨慢性丙型肝炎患者血清白介素-8（IL-8）水平与丙型肝炎病毒(HCV)感染、基因型及抗病毒治疗的关系。方法对74例慢性丙型肝炎患者应用基因芯片法进行基因型检测，应用双抗体夹心ABC- ELISA法检测患者血清IL-8。比较不同基因型患者之间IL-8水平的差别，观察了抗病毒治疗12周后IL-8水平的变化。结果慢性丙型肝炎患者及健康对照组血清IL-8水平分别为(42.95±50.00) pg/mL及(11.06 ± 1.39 )pg/mL（P＜0.01）；基因1b型慢丙肝患者（46例）及基因2a型患者（28例）IL-8水平分别为(52.72±60.67 )pg/mL及(26.93±13.97 )pg/mL（P＜0.05）。抗病毒治疗后HCV RNA转阴者，其IL8水平由(41.20±25.89 )pg/mL 降至(23.89± 10.59 )pg/mL（P＜0.01）。结论慢丙肝患者血清IL-8水平较健康对照组明显升高，且以感染HCV基因1b型者更明显。抗病毒治疗HCV RNA阴转后，其IL-8水平明显回降。

关键词: 肝炎，丙型，慢性；白介素8；基因型；抗病毒治疗

Abstract:

Objective To study the relationship of expression level of serum interleukin-8 (IL-8) and HCV genotypes in patients with chronic hepatitis C and observe the change of serum IL-8 level after antivirus treatment. Methods HCV genotypes were determined using DNA chip assay in 74 chronic hepatitis C patients. Serum IL-8 of these patients was determined using bi-antibody sandwich ABC-ELISA assay. Levels of IL-8 in chronic hepatitis C patients with different genotypes were compared. The change of IL8 level after 12 weeks of antivirus treatment was observed. Results Levels of serum IL-8 in chronic hepatitis C patients and healthy controls were (42.95±50.00)pg/mL and( 11.06±1.39)pg/mL, respectively（P＜0.01），and in patients infected with genotype 1b and genotype 2a were ( 52.72±60.67)pg/mL and (26.93±13.97)pg/mL, respectively（P＜0.05）. After 12 weeks of antivirus treatment, the serum IL-8 level in patients with undetectable HCV RNA decreased from (41.20±25.89)pg/mL to (23.89±10.59 )pg/mL（P＜0.01）. Conclusions The serum level of IL-8 in patients with chronic hepatitis C significantly increases compared with that in healthy controls. The IL8 level in patients with genotype 1b is higher than that in patients with genotype 2a. The IL-8 level in patients with undetectable HCV RNA significantly decreases after antivirus treatment.

Key words: Hepatitis C, Chronic; Interleukin8; Genotype; Antivirus treatment

中图分类号:

512.6

张立新1，2，安勇2，徐皖苏3，张孝国2，刘靓雯3，于进红2，张琴冈2. 慢性丙型肝炎患者血清白介素-8表达水平与基因型及抗病毒治疗的相关性[J]. 山东大学学报(医学版), 2010, 48(8): 100-102.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed