MAPK在弗司可林诱发的脊髓背角LTP中的作用

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (6): 53-56.

MAPK在弗司可林诱发的脊髓背角LTP中的作用

杨红卫

三峡大学医学院生理学教研室，湖北宜昌 443002

收稿日期:2009-10-27 出版日期:2010-06-16 发布日期:2010-06-16
作者简介:杨红卫（1968- ），男，副教授, 主要从事病理性疼痛的中枢和外周机制及脂类活性物质在突触传递与可塑性中的作用机制研究。Email: yhw0224@.yahoo.com
基金资助:
湖北省卫生厅科研基金资助项目（No.JX2B77），湖北省教育厅自然科学基金资助项目（No.Q200613002）。

Role of MAPK in the induction of LTP of C-fiber-evoked field potentials in spinal dorsal horn induced by Forskolin

YANG Hongwei

Department of Physiology, School of Medicine, China Three Gorges University, Yichang 443002, Hubei, China

Received:2009-10-27 Online:2010-06-16 Published:2010-06-16

摘要/Abstract

摘要：

目的探讨丝裂霉素活化蛋白（MAPK）在弗司可林（Forskolin）诱发的脊髓背角长时程增强（LTP）中的作用。方法采用SD 大鼠, 常规细胞外记录技术记录脊髓背角腰膨大部位浅层C-纤维诱发电位。结果 ①弗司可林（100μmol/L）诱发的脊髓背角LTP为PKA的抑制剂H89 (200μmol/L) 所阻断；②MAPK的选择性抑制剂PD98059 (100μmol/L) 阻断弗司可林诱导的脊髓 LTP； ③NMDA受体抑制剂阻断弗司可林诱导的脊髓 LTP。结论 MAPK参与弗司可林诱导的脊髓背角C-纤维诱发的LTP；弗司可林诱导的LTP可能是通过cAMP-PKA-MAPK信号途径起作用的。

关键词: 丝裂原活化蛋白激酶；弗司可林；长时程增强；脊髓背角；病理性疼痛

Abstract:

Objective To evaluate the role of mitogen-activated protein kinase (MAPK) in the induction of spinal long-term potentiation (LTP) induced by Forskolin. Methods The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement. Results ① Forskolin (100μmol/L)induced LTP of C-fiber evoked field potentials was blocked by H89 (a PKA inhibitor). ② Specific inhibitor of MAPK PD98059 blocked the induction of spinal LTP by Forskolin. ③ The synaptic potentiation induced by Forskolin was blocked in the presence of either MK-801 or APV, inhibitors of NMDA receptor. Conclusions MAPK is involved in the induction of LTP of C-fiber evoked field potentials in spinal dorsal horn by Forskolin. Forskolin-induced effect might be mediated via cAMP-PKA-MAPK signaling pathway.

Key words: Mitogen-activated protein kinase； Forskolin; Long-term potentiation; Spinal dorsal horn; Pathological pain

中图分类号:

R338.2

杨红卫. MAPK在弗司可林诱发的脊髓背角LTP中的作用[J]. 山东大学学报(医学版), 2010, 48(6): 53-56.

YANG Hongwei. Role of MAPK in the induction of LTP of C-fiber-evoked field potentials in spinal dorsal horn induced by Forskolin[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 53-56.

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