您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (5): 99-102.

• 论文 • 上一篇    下一篇

溃疡性结肠炎激素敏感性与多药耐药基因多态性的相关性研究

李玲,陈勇,杨崇美   

  1. 山东大学附属省立医院消化内科, 济南 250021
  • 收稿日期:2011-11-08 出版日期:2012-05-10 发布日期:2012-05-10
  • 通讯作者: 杨崇美(1964- ),女,博士,主任医师,教授,主要从事炎症性肠病研究。 E-mail:yangchongmei@medmail.com
  • 作者简介:李玲(1984- ),女,硕士研究生,主要从事溃疡性结肠炎的基础研究
  • 基金资助:

    山东省自然科学基金资助项目(NO.Y2006C135)。

Correlation study of single nucleotide polymorphism of multiple
drug resistance 1 and ulcerative colitis prednisone sensitivity

LI Ling, CHEN Yong, YANG Chong-mei   

  1. Department of Gastroenterology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2011-11-08 Online:2012-05-10 Published:2012-05-10

摘要:

目的   探讨溃疡性结肠炎患者激素治疗敏感性与多药耐药基因(MDR1)多态性之间的相关性。方法   研究对象为入院治疗的86例溃疡性结肠炎患者,根据入院时的病史采集将86例患者分为3组:溃疡性结肠炎患者初诊组30例、溃疡性结肠炎患者激素治疗敏感型组30例、溃疡性结肠炎患者激素治疗耐药型组26例。采用序列特异性聚合酶链反应(PCR-SSP)进行基因多态性分析,测定86例溃疡性结肠炎患者MDR1基因C3435T位点及G2677T/A位点的基因型频率和等位基因频率,并进行统计分析。结果   溃疡性结肠炎患者激素治疗耐药型组与初诊组C3435T位点CC、CT、TT基因型的差异具有统计学意义,激素治疗耐药型组与激素治疗敏感型组C3435T位点CC、CT、TT基因型的差异也具有统计学意义。3组患者C3435T位点等位基因频率的差异均无统计学意义。而对于MDR1基因G2677T/A位点,各组基因型的差异及G、T、A等位基因频率的差异均无统计学意义。结论   溃疡性结肠炎患者激素治疗耐药型与MDR1基因C3435T位点多态性之间存在相关性,该位点基因突变型患者更易出现激素耐药。

关键词: 溃疡性结肠炎; MDR1基因C3435T、G2677T/A多态性; 激素敏感性; P糖蛋白

Abstract:

Objective   To investigate the relationship between multiple drug resistance 1(MDR1) polymorphism and prednisone sensitivity in ulcerative colitis (UC) patients. Methods   86 UC patients were divided into three groups according to their history: 30 newly diagnosed UC patients, 30 UC patients who were previously sensitive to the prednisone treatment and 26 UC patients on whom the prednisone treatment had no effect. DNA samples were obtained from 86 UC patients. Genotypes of the MDR1C3435T and G2677T/A loci were determined by polymerase chain reaction of the sequence specific primer (PCR-SSP). Results   The MDR1C3435T locus consisted of three genotypes: CC,CT and TT. There were significant differences between the newly diagnosed UC patients and prednisone resistant patients in the MDR1C3435T locus. The same results occurred in prednisone resistance patients and prednisone sensitive patients. However there was no significant difference in C and T allele frequencies among the three groups in the MDR1C3435T locus. There was no significant difference of the genotypes and allele frequencies of the G2677T/A locus among the three groups. Conclusion   There is a relationship between the MDR1C3435T polymorphism and prednisone resistance UC patients in Chinese Han nationality. The MDR1C3435T gene mutation in UC patients is prone to the prednisone resistance. The result can not prove there is a correlation between UC in Chinese Han nationality and MDR1 G2677T/A polymorphism.

Key words: Ulcerative colitis; MDR1C3435T and G2677T/A gene polymorphism; Prednisone sensitivity; P-glycoprotein

中图分类号: 

  • R574.62
[1] 辛玉1,袁俊华2,孙成刚1. EGCG抑制结肠癌HT-29细胞生长及血管生成的作用机制[J]. 山东大学学报(医学版), 2013, 51(3): 21-26.
[2] 孟宪邑,张芸,吴震州. 娃儿藤碱类化合物DCB-3503对TNBS诱导溃疡性结肠炎的治疗作用[J]. 山东大学学报(医学版), 2012, 50(10): 81-.
[3] 程兆令,李雪梅,杨廷廷,朱强. 结直肠癌中TLR4的表达及其与FasL、EGFR的关系[J]. 山东大学学报(医学版), 2012, 50(5): 92-.
[4] 马铭泽,张安忠,蒯景华,杨崇美. 溃疡性结肠炎患者食物特异性抗体的检测和剔除过敏食物对临床疗效的影响[J]. 山东大学学报(医学版), 2011, 49(1): 86-89.
Viewed
Full text
73
HTML PDF
Just accepted Online first Issue Just accepted Online first Issue
0 0 0 0 0 73

  From Others local
  Times 8 65
  Rate 11% 89%

Abstract
587
Just accepted Online first Issue
0 0 587
  From Others
  Times 587
  Rate 100%

Cited

Web of Science  Crossref   ScienceDirect  Search for Citations in Google Scholar >>
 
This page requires you have already subscribed to WoS.
  Shared   
  Discussed   
No Suggested Reading articles found!