结直肠癌中TLR4的表达及其与FasL、EGFR的关系

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (5): 92-.

结直肠癌中TLR4的表达及其与FasL、EGFR的关系

程兆令，李雪梅，杨廷廷，朱强

山东大学附属省立医院消化内科，济南 250021

收稿日期:2011-10-08 出版日期:2012-05-10 发布日期:2012-05-10
通讯作者: 朱强，男，主任医师，主要从事结肠癌及肝纤维化的研究。 E-mail: zhuqiangmayo@gmail.com
作者简介:程兆令(1985- )，男，硕士研究生，主要从事结肠癌的研究
基金资助:
山东省优秀中青年科学家奖励基金(BS2009YY022)。

Expression of TLR 4 in colorectal cancer and its
relationships with FasL and EGFR

CHENG Zhao-ling, LI Xue-mei, YANG Ting-ting, ZHU Qiang

Department of Gastroenterology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2011-10-08 Online:2012-05-10 Published:2012-05-10

摘要/Abstract

摘要：

目的观察Toll样受体4(tolllike receptor 4，TLR4)在结直肠癌和正常大肠组织中的表达情况，并且分析其表达与Fas配体 (fas ligand，FasL)和表皮生长因子受体(epidermal growth factor receptor, EGFR) 表达的关系。方法采用免疫组化SP三步法检测58例结直肠癌和25例正常大肠组织TLR4、FasL和EGFR的表达。通过Spearman′s相关分析，探讨TLR4与FasL或EGFR表达的关系。结果 58例结直肠癌中TLR4、FasL和EGFR表达的阳性率分别为59%、71% 和83%，均显著高于其在正常大肠组织中的表达阳性率20%、22%和31%(P<0.05)。TLR4的表达与结直肠癌的远处转移具有相关性(χ2 =4.063，P＜0.05)。TLR4和FasL表达具有正相关性(r=0.249，P<0.01)，TLR4表达强的部位多伴有FasL的强表达，TLR4表达弱的部位也常伴随FasL的弱表达。TLR4与EGFR的表达不具有相关性(r=0.345，P>0.05)。结论 TLR4的表达与结直肠癌远处转移有关，TLR4可能通过调控FasL的表达而参与结直肠癌的免疫反击。

关键词: Toll样受体4；Fas配体；表皮生长因子受体；结直肠肿瘤；免疫组织化学

Abstract:

Objective To investigate expression of tolllike receptor 4 (TLR4), fas ligand (FasL) and epidermal growth factor receptor (EGFR) in colorectal cancer and normal intestinal tissues. Methods SP immunohistochemistry was used to study expression of TLR4, FasL and EGFR in 58 colorectal tissues and 25 normal colorectal tissues (control). Results Expression rates of TLR4, FasL and EGFR were 59%, 71% and 83% in colorectal cancers and 20%, 21% and 23% in normal intestinal tissues, respectively. TLR4 expression was correlated with distant metastasis of colorectal tumors(χ2 =4.063， P＜0.05). TLR4, FasL and EGFR all exhibited significantly higher expression in colorectal cancers than in normal tissues. There was a positive correlation between TLR4 and FasL in their expression rates(r=0.249， P<0.01). Between TLR4 and EGFR, there was no apparent correlation(r=0.345， P>0.05). Conclusions High expression of TLR4 is correlated with distant metastasis of colorectal tumors and may boost the immune counterattack of colorectal cancer cells through up-regulating expression of FasL.

Key words: Toll-like receptor 4； Fas ligand； Epidermal growth factor receptor； Colorectal neoplasms; ImmunohistochemistryToll

中图分类号:

R574.62

程兆令，李雪梅，杨廷廷，朱强. 结直肠癌中TLR4的表达及其与FasL、EGFR的关系[J]. 山东大学学报(医学版), 2012, 50(5): 92-.

CHENG Zhao-ling, LI Xue-mei, YANG Ting-ting, ZHU Qiang. Expression of TLR 4 in colorectal cancer and its
relationships with FasL and EGFR[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 92-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	辛玉1，袁俊华2，孙成刚1. EGCG抑制结肠癌HT-29细胞生长及血管生成的作用机制[J]. 山东大学学报(医学版), 2013, 51(3): 21-26.
[2]	孟宪邑，张芸，吴震州. 娃儿藤碱类化合物DCB-3503对TNBS诱导溃疡性结肠炎的治疗作用[J]. 山东大学学报(医学版), 2012, 50(10): 81-.
[3]	李玲，陈勇，杨崇美. 溃疡性结肠炎激素敏感性与多药耐药基因多态性的相关性研究[J]. 山东大学学报(医学版), 2012, 50(5): 99-102.
[4]	马铭泽,张安忠,蒯景华,杨崇美. 溃疡性结肠炎患者食物特异性抗体的检测和剔除过敏食物对临床疗效的影响[J]. 山东大学学报(医学版), 2011, 49(1): 86-89.