HepaCAM在肾透明细胞癌中的表达及临床意义

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (3): 81-84.

HepaCAM在肾透明细胞癌中的表达及临床意义

刘海南1,2，李大伟2，张建平3，任巨超2，田素建2，房志卿2，吕家驹1

山东大学 1.附属省立医院泌尿外科，济南 250021； 2.齐鲁医院泌尿外科，济南 250012；
3.齐鲁医院病理科，济南 250012

收稿日期:2010-11-29 出版日期:2011-03-10 发布日期:2011-03-10
通讯作者: 吕家驹(1964- )，男，博士生导师，教授，主要从事泌尿系肿瘤和男科学研究。 E-mail: kyoto2310 @sina.com
作者简介:刘海南(1962- )，男，博士研究生，副教授，主要从事泌尿系结石及泌尿系肿瘤的基础与临床研究
基金资助:
山东省科学技术发展计划资助项目（032050166）。

Expression and clinical significance of HepaCAM expression in renal clear cell carcinoma

LIU Hai-nan1,2, LI Da-wei2, ZHANG Jian-ping3, REN Ju-chao2, TIAN Su-jian2, FANG Zhi-qing2, L Jia-ju1

1. Department of Urologic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
2. Department of Urologic Surgery, Qilu Hospital of Shandong University, Jinan 250012, China;
3. Department of Pathology, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2010-11-29 Online:2011-03-10 Published:2011-03-10

摘要/Abstract

摘要：

目的检测肾透明细胞癌（RCCC）组织中肝细胞黏附因子（HepaCAM）的表达水平，探讨HepaCAM在肾透明细胞癌发生、发展过程中的意义。方法选取73例肾透明细胞癌组织和12例癌旁组织作为研究对象，采用非生物素即用型二步法（PV9001）免疫组织化学方法检测组织中HepaCAM蛋白的表达，运用Image-Pro Plus 6.0图像分析软件测定免疫组化图像的累积光密度值（IOD），应用SPSS13.0软件进行统计分析。结果 HepaCAM蛋白在RCCC组织中低表达，而在癌旁组织中高表达，IOD分别为8203.87±8431.88和36639.28±20851.94，差异具有统计学意义（P<0.05）。而HepaCAM蛋白的表达与性别、年龄、肿瘤大小、T分期和TNM分期等临床特征均无相关关系。结论 HepaCAM蛋白在癌组织中低表达，可能与肾透明细胞癌的发生有密切关系，可作为判断肿瘤发生的指标。

关键词: 肾透明细胞癌；肝细胞黏附因子；免疫组织化学

Abstract:

Objective To detect expression of hepatocyte cell adhesion molecule(HepaCAM) in renal clear cell carcinoma (RCCC), and to study the relativity of HepaCAM expression with occurrence and development of RCCC. Methods Paraffin-embedded tissue sections from 73 patients with RCCC and 12 RCCC adjacent renal tissues were included in the study. Expression of HepaCAM in all the samples was analyzed with the immunohistochemical(PV-9001) method. Integrated optical density(IOD) of the immunohistochemical image was estimated by Image-ProPlus 6.0 software, and the correlation of IOD and clinical features of RCCC was analyzed. Statistical analysis was performed by SPSS13.0 software. Results HepaCAM expression was down-regulated in RCCC, while it was up-regulated in RCCC adjacent tissues. IOD in the two groups was 8203.87±8431.88 and 36639.28±20851.94, respectively(P<0.05). Expression of HepaCAM had no relation with clinical features such as gender, age, the tumor size, the T stage and TNM stage. Conclusions HepaCAM expression is lower in RCCC compared with RCCC adjacent tissues, and may has a close relation with the development of RCCC. HepaCAM is supposed to act as a marker for carcinogenesis in the kidney.

Key words: Renal clear cell carcinoma; Hepatocyte cell adhesion molecule; Immunohistochemistry

中图分类号:

R737.11

刘海南1,2，李大伟2，张建平3，任巨超2，田素建2，房志卿2，吕家驹1. HepaCAM在肾透明细胞癌中的表达及临床意义[J]. 山东大学学报(医学版), 2011, 49(3): 81-84.

LIU Hai-nan1,2, LI Da-wei2, ZHANG Jian-ping3, REN Ju-chao2, TIAN Su-jian2, FANG Zhi-qing2, L Jia-ju1. Expression and clinical significance of HepaCAM expression in renal clear cell carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 81-84.

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