不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (3): 109-112.

不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用

王灿，苏良，沈菲，陈士俊

山东大学济南市传染病医院四科，济南 250021

收稿日期:2009-12-27 出版日期:2010-03-16 发布日期:2010-03-16
通讯作者: 陈士俊(1957- )，男，主任医师，主要从事病毒性肝炎研究。
作者简介:王灿(1979- )，女，硕士研究生，主治医师,主要从事病毒性肝炎研究。

Roles of different subtypes of CD4+ T lymphocytes in IFN-α therapy for hepatitis B

WANG Can， SU Liang， SHEN Fei， CHEN Shi-jun

Department 4, Jinan Infectious Disease Hospital, Shandong University, Jinan 250021, China

Received:2009-12-27 Online:2010-03-16 Published:2010-03-16

摘要/Abstract

摘要：

目的探讨不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用。方法采用酶联免疫吸附试验（ELISA）法测定54例慢性乙型肝炎患者应用普通干扰素治疗（500万单位，隔日皮下注射1次）前和治疗24周后血清中辅助性T淋巴细胞（Th）17、1、2分泌的主要细胞因子白细胞介素17（IL-17）、γ干扰素（IFN-γ）、白细胞介素4（IL-4）的水平，并根据应答的不同进行分组，分析不同亚型CD4+T淋巴细胞的作用。结果慢性乙型肝炎患者经普通干扰素治疗24周后IL-17水平下降（P＞0.05），IFNγ水平上升(P＞0.05)，IL4水平明显下降(P＜0.01)。而在干扰素治疗的完全应答组，IL-17、IL-4水平下降更明显(P＜0.05、P＜0.01 )，IFNγ水平则明显升高(P＜0.01)。结论慢性乙型肝炎患者经干扰素抗病毒治疗，体内Th17和Th2的功能下调，Th1功能上调。

关键词: 肝炎，乙型，慢性；α干扰素；辅助性T淋巴细胞

Abstract:

Objective To study the roles of different subtypes of CD4+ T lymphocytes in IFN-α therapy for hepatitis B. Methods Th17, Th1 and Th2 cytokines were determined by enzymelinked immunosorbent assay(ELISA) in 54 hepatitis B patients before and 24 weeks after IFNα treatment. Patients were grouped according to responses to IFN-α therapy. The data were analyzed by t-test and Pearson correlation analysis. Results The level of IL-17 was slightly lower 24 weeks after IFN-α treatment than that at pretreatment(P＞0.05); the level of IFN-γ was slightly increased(P＞0.05); and the level of IL-4 was substantially decreased(P＜0.01). In patients with a complete response to IFNα, the level of IFNγ was obviously increased(P＜0.01); and the levels of IL-17 and IL-4 were significantly decreased(P＜0.05; P＜0.01). Conclusion During IFN-α therapy, the functions of Th17 and Th2 are down-regulated and the function of Th1 is up-regulated in chronic hepatitis B.

Key words: Hepatitis B, chronic; Interferon-α; Helper T cell

中图分类号:

R392.1

王灿，苏良，沈菲，陈士俊. 不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用[J]. 山东大学学报(医学版), 2010, 48(3): 109-112.

WANG Can， SU Liang， SHEN Fei， CHEN Shi-jun. Roles of different subtypes of CD4+ T lymphocytes in IFN-α therapy for hepatitis B[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(3): 109-112.

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