γ-干扰素、白细胞介素-10在口腔扁平苔藓上皮下结缔组织中的表达

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (1): 138-141.

γ-干扰素、白细胞介素-10在口腔扁平苔藓上皮下结缔组织中的表达

潘燕1，包琪2，左志彬3，戚向敏1

1. 山东大学口腔医学院，济南 250012；
2. 山东大学口腔生物医学实验室，济南 250012； 3. 山东省济南市口腔医院口腔内科，济南 250001

收稿日期:2009-04-01 出版日期:2010-01-16 发布日期:2010-01-16
通讯作者: 戚向敏（1969- ），女，教授，主要从事口腔粘膜病学研究。
作者简介:潘燕（1983- ），女，硕士研究生，主要从事口腔粘膜病学研究。
基金资助:
山东省自然科学基金资助项目（Y2007C095）

Expressions of IFN-γ and IL-10 in sub-epithelial connective tissues of oral lichen planus

PAN Yan1, BAO Qi2，　ZUO Zhibin3,　QI Xiangmin1

1. School of Dentistry, Shandong University, Jinan 250012, China;
2. Lab of Oral Biomedicine, School of Dentistry, Shandong University, Jinan 250012, China;  3. Department of Oral Medicine of Jinan Dental Hospital, Jinan 250001, China

Received:2009-04-01 Online:2010-01-16 Published:2010-01-16
Contact: 戚向敏（1969- ），女，教授，主要从事口腔粘膜病学研究

摘要/Abstract

摘要：

目的检测口腔扁平苔藓黏膜上皮下结缔组织中γ-干扰素（IFN-γ）、白细胞介素-10（IL-10）的表达情况，初步探讨其在口腔扁平苔藓发生发展过程中的作用及意义。方法应用PCR技术和荧光实时定量PCR技术检测11例口腔扁平苔藓黏膜上皮下结缔组织和5例正常口腔黏膜上皮下结缔组织中IFN-γ、IL-10的表达情况。结果荧光实时定量PCR检测结果显示，口腔扁平苔藓黏膜上皮下结缔组织中IFN-γmRNA的表达值实验组与对照组分别为0.2981±0.2064和0.0986±0.0894，扁平苔藓组织均高于正常口腔黏膜组织；IL-10 mRNA的表达值实验组与对照组分别为4.3218±4.4678和15.1156±9.1392，扁平苔藓组织均低于正常口腔黏膜组织，经检验差异有统计学意义(P＜0.05)。结论与正常口腔黏膜组织相比，口腔扁平苔藓组织中具有促炎作用的IFN-γ表达水平增高，而具有抗炎作用的IL-10表达水平降低，提示抗炎症反应和促炎症反应在口腔扁平苔藓的发生发展过程中起到一定的作用。

关键词: 细胞因子；扁平苔藓,口腔；荧光实时定量PCR

Abstract:

Objective To detect expressions of IFN-γ and IL-10 in sub-epithelialconnective tissues of oral lichen planus (OLP) and to explore their roles in the occurrence and process of OLP. Methods Specimens were collected from 11 patients with OLP and 5 normal oral mucosa. Expressions of IFN-γ and IL-10 were determined by polymerase chain reaction (PCR) technology and fluorescent real-time quantitative PCR technology. Results Fluorescent real-time quantitative PCR results showed that expression of IFN-γ mRNA in OLP and in normal oral mucosa was 0.2981±0.2064 and 0.0986±0.0894, while expression of IL-10 mRNA in OLP and in normal oral mucosa was 4.3218±4.4678 and 15.1156±9.139?2. Conclusion Compared with normal oral mucosa, IFN-γ expression was increased, however, IL-10 expression was reduced in OLP, suggesting that an anti-inflammatory response and pro-inflammatory response had some role in the occurrence and process of OLP.

Key words: Cytokines; Lichen planus, oral; Fluorescent real-time quantitative polymerase chain reaction

中图分类号:

R781.5+9

潘燕1，包琪2，左志彬3，戚向敏1. γ-干扰素、白细胞介素-10在口腔扁平苔藓上皮下结缔组织中的表达[J]. 山东大学学报(医学版), 2010, 48(1): 138-141.

PAN Yan1, BAO Qi2，　ZUO Zhibin3,　QI Xiangmin1. Expressions of IFN-γ and IL-10 in sub-epithelial connective tissues of oral lichen planus[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(1): 138-141.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed