基因芯片技术分析胎盘血管病变中差异基因的表达

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (1): 131-134.

基因芯片技术分析胎盘血管病变中差异基因的表达

刘彦霞1，2，张峰2，王欣1

山东大学附属省立医院 1. 血液科； 2. 保健综合科，济南 250021

收稿日期:2009-06-10 出版日期:2010-01-16 发布日期:2010-01-16
通讯作者: 王欣，教授，博士生导师。
作者简介:刘彦霞（1979- ），女，博士研究生，主治医师，主要从事血液病的诊治。
基金资助:
国家自然科学基金资助课题（30471825）

Expression of differential genes in placental vascular disease and normal placenta through gene arrays

LIU Yanxia1，2， ZHANG Feng2， WANG Xin1

1. Department of Hematology； 2. Department of General Medicine， Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2009-06-10 Online:2010-01-16 Published:2010-01-16

摘要/Abstract

摘要：

目的利用基因芯片技术分析胎盘血管病变差异基因的表达，为深入探讨胎盘血管病变的发生机制提供新的线索。方法应用22?K人类基因组寡核苷酸芯片，筛选了胎盘血管病变患者（实验组）和正常妊娠者（对照组）胎盘组织中的差异表达基因，应用定量聚合酶链反应（PCR）及蛋白印记分析（Western-blot）验证部分差异基因的表达。结果实验组与对照组比较，存在10个表达水平显著改变的基因，其中9个在胎盘血管病变中上调，1个下调。经验证部分差异基因的表达与芯片结果一致。结论胎盘血管病变是一种具有血管壁内皮细胞激活和免疫应答的炎性疾病。这些差异表达基因可能通过多种信号转导途径参与了内皮激活与炎症应答，并导致了胎盘血管病变的发生和发展。

关键词: 胎盘血管病变；基因芯片；差异基因

Abstract:

Objective To further approach a new clue for the mechanism of placental vascular diseases(PVD) by performing a gene expression profiling study in placenvascular disease through gene array.Methods We performed a gene expressionprofiling study of placental tissues from women with and without PVD by using 22k Human Genome Oligo Array and screened the different expressive genes between PVD and controls. Then real-time quantitative polymerase chain reaction (PCR) and Western-blot were used to verify expression of some different genes. Results By a comparative differential gene expression analysis between the two groups, we found 10 genes to be significantly altered in their expression levels in PVD. Among them 9 genes were up-regulated and 1 gene was down-regulated in PVD. Theverification test result was coincident with the gene array result. Conclusion Placental vascular disease is an inflammatory disease characterized by activation of endothelial cells and immune response in the vessel wall. These genes can participate in endothelial activation and inflammatory response through activation of the transcription factors, which may result in the occurrence and development of PVD.

Key words: Placental vascular diseases; Gene arrays; Differential genes

中图分类号:

R714.2

刘彦霞1，2，张峰2，王欣1. 基因芯片技术分析胎盘血管病变中差异基因的表达[J]. 山东大学学报(医学版), 2010, 48(1): 131-134.

LIU Yanxia1，2， ZHANG Feng2， WANG Xin1. Expression of differential genes in placental vascular disease and normal placenta through gene arrays[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(1): 131-134.

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