妊娠早期人外周血及蜕膜局部单核细胞异质性分析

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (3): 98-101.

妊娠早期人外周血及蜕膜局部单核细胞异质性分析

张韵1，2，孙锦堂2，黄先亮3，杨美香2，邵倩倩2，邓碧萍2，徐小飞3，毛海婷2，董白桦3，孔北华3，曲迅1，2

1. 山东省医学科学院基础医学研究所，济南 250062； 2. 山东大学齐鲁医院临床基础研究所，济南 250012；
3. 山东大学齐鲁医院妇产科，济南 250012

收稿日期:2009-11-10 出版日期:2010-03-16 发布日期:2010-03-16
通讯作者: 曲迅，教授，博士生导师，主要从事生殖免疫学、免疫调控与肿瘤免疫学研究。
作者简介:张韵(1983- )，男，硕士研究生，主要从事生殖免疫学研究。
基金资助:
国家自然科学基金资助课题(30872321,30671902)；山东省自然科学基金资助课题（Y2008C02）。

Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy

ZHANG Yun 1,2, SUN Jin-tang 2, HUANG Xian-liang 3, YANG Mei-xiang 2,SHAO Qian-qian 2, DENG Bi-ping 2, XU Xiao-fei 3,MAO Hai-ting 2, DONG Baihua-3, KONG Bei-hua 3, QU Xun 1，2

1. Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, China; 
2. Basic Medical Science Institute, Qilu Hospital of Shandong University, Jinan 250012, China; 
3. Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2009-11-10 Online:2010-03-16 Published:2010-03-16

摘要/Abstract

摘要：

目的分析早孕妇女外周血及蜕膜局部CD14+CD16+/CD14+CD16-单核细胞各亚群的分布，并初步探讨其临床意义。方法随机抽取健康早孕者及健康未孕者外周血，取人工流产术获得的健康早孕妇女新鲜蜕膜组织，采用机械研磨及密度梯度离心法分离单个核细胞;流式细胞术检测上述样本CD14+CD16+/CD14+CD16-单核细胞各亚群的分布。结果与健康未孕者比较，早孕妇女外周血CD14+CD16-细胞阳性率显著降低（P＜0.01），早孕蜕膜组织中CD14+CD16+细胞亚群为CD14+细胞的主要群体，阳性率为(69.13±7.73) %，显著高于外周血中该亚群（11.50±3.7）%的比例。结论早孕期间母体CD14+CD16+细胞亚群成为CD14+细胞中的主群体，提示CD14+CD16+细胞亚群在妊娠期间免疫系统TH-2优势应答的形成中可能起重要作用。

关键词: 妊娠初期；外周血；蜕膜；单核细胞

Abstract:

Objective To analyze the distribution of human CD14+CD16- and CD14+CD16+ monocyte subsets in peripheral blood and decidua of women in early pregnancy. Methods Peripheral blood samples were collected from early pregnant and nonpregnant females. Mononuclear cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation. The fluorescent intensity of the cells was detected by FCM. Results The percentage of the peripheral CD14+CD16- monocyte subtype in early pregnant females was lower than that in non-pregnant controls(P＜0.01). CD14+CD16+ cells (69.13%±7.73%) and were the major monocyte subset in the decidua of early pregnant females. Conclusion CD14+CD16+ monocytes become the predominant cell subpopulation of women in early pregnancy. It has been demonstrated that CD14+CD16+ monocyte-derived dendritic cells are efficient producers of anti-inflammatory interleukin-10, so CD14+CD16+ monocytes might play an important role in forming and keeping the dominance of TH2 cytokine during early pregnancy.

Key words: First pregnancy trimester; Peripheral blood; Decidua; Monocyte

中图分类号:

R714.14

张韵1，2，孙锦堂2，黄先亮3，杨美香2，邵倩倩2，邓碧萍2，徐小飞3，毛海婷2，董白桦3，孔北华3，曲迅1，2. 妊娠早期人外周血及蜕膜局部单核细胞异质性分析[J]. 山东大学学报(医学版), 2010, 48(3): 98-101.

ZHANG Yun 1,2, SUN Jin-tang 2, HUANG Xian-liang 3, YANG Mei-xiang 2,SHAO Qian-qian 2, DENG Bi-ping 2, XU Xiao-fei 3,MAO Hai-ting 2, DONG Ba. Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(3): 98-101.

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