虎杖免煎冲剂对糖尿病大鼠肾脏RAGE及VEGF表达的影响

山东大学学报(医学版)

虎杖免煎冲剂对糖尿病大鼠肾脏RAGE及VEGF表达的影响

费洪华¹,肖正大²,高薇³

1. 山东大学医学院, 济南 250012; 2. 济南市中心医院内分泌科, 济南 250013;3. 济南市中心医院病理科, 济南 250013

收稿日期:2007-07-14 修回日期:1900-01-01 出版日期:2008-01-16 发布日期:2008-01-16
通讯作者: 肖正大

Effects of giant knotweed rhizome medicine on the expressions of RAGE and VEGF of rats with diabetic nephropat

FEI Hong-hua¹,XIAO Zheng-da²,GAO Wei³

1. School of Medicine, Shandong University, Jinan 250012, China;2. Department of Endocrinology, Jinan Central Hospital, Jinan 250013, China;3. Department of Pathology, Jinan Central Hospital

Received:2007-07-14 Revised:1900-01-01 Online:2008-01-16 Published:2008-01-16
Contact: XIAO Zheng-da

摘要/Abstract

摘要： 目的探讨虎杖免煎冲剂对实验性糖尿病大鼠肾脏病理结构和糖基化终末产物受体(RAGE)、血管内皮生长因子(VEGF)表达的影响。方法Wistar大鼠36只,其中8只喂养普通饲料(正常组)，其余28只给予高糖高脂饲料喂养,4周后注射链脲佐菌素(STZ)造成糖尿病模型,造模成功后，分别用蒸馏水(模型组)、虎杖免煎冲剂(虎杖组)、氟伐他汀(他汀组)灌胃。8周后观察各生化指标的变化;HE染色及电镜下观察肾脏病理改变;免疫组化法检测肾小球RAGE、VEGF的表达水平。结果与模型组相比,虎杖组大鼠的肾重／体重、肌酐清除率、24h尿蛋白量、肾小球平均体积均下降(P＜0.01,P＜0.05),肾脏病变程度轻,肾小球RAGE、VEGF表达下调(P＜0.05,P＜0.01)。结论虎杖免煎冲剂对糖尿病大鼠具有肾脏保护作用。其机制可能与其下调肾组织中RAGE、VEGF的表达有关。

Abstract: ObjectiveTo investigate the effects of giant knotweed rhizome medicine on the pathological changes and expressions of receptor for advanced glycation end products (RAGE)、 and vascular endothelial growth factor (VEGF) in nephridial tissues in experimental diabetic rats. Methods36 healthy male Whistar rats were divided into the control group(n=8) and the experimental group (n=28) which were given diets enriched with sucrose(20％w／w), lard(10％ w／w), cholesterol(2.5％ w／w) and cholic acid(0.5％ w／w). After 4 weeks, diabetic rat models were induced by an injection of streptozotocin (STZ) into the vena caudalis and were randomly divided into 3 groups: the model group(fed with distilled water), the giant knotweed rhizome medicine group and the fluvastatin group. Blood glucose(BG), KW／BW(kidney weight／body weigh), creatinine clearance (Ccr), and 24h urinary protein (24 hUP) were determined after 8 weeks. Pathological changes of the kidneys were observed by using light and electron microscopy, and the expressions of receptors for advanced glycation end products(RAGE)and vascular endothelial growth factor(VEGF) in the glomcrulus were determined by using immunohistochemical methods. ResultsAfter interference, compared with the model group, the KW／BW, Ccr, 24?hUP, and the volume of the glomcrulus of the giant knotweed rhizome medicine group were significantly downregulated(P＜0.01 and P＜0.05), also expressions of RAGE and VEGF in the glomcrulus were significantly inhibited(P＜0.05 and P＜0.01). ConclusionGiant knotweed rhizome medicine has a renoprotective effect for diabetic rats, which may relate to the downregulation of expressions of RAGE and VEGF in nephridial tissues.

Key words: Diabetic nephropathy, Giant knotweed rhizome medicine, Fluvastatins, Receptor for advanced glycation end products, Vascular endothelial growth factor, Rats, Wistar

中图分类号:

R965

费洪华,肖正大,高薇 . 虎杖免煎冲剂对糖尿病大鼠肾脏RAGE及VEGF表达的影响[J]. 山东大学学报(医学版), 2008, 46(1): 43-47.

FEI Hong-hua,XIAO Zheng-da,GAO Wei. Effects of giant knotweed rhizome medicine on the expressions of RAGE and VEGF of rats with diabetic nephropat[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(1): 43-47.

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