尿毒清对慢性肾功能衰竭大鼠肾脏纤维化的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (3): 57-60.

尿毒清对慢性肾功能衰竭大鼠肾脏纤维化的影响

亓敏1，梁素忍1，王娜1，甄军晖2，冯进波3，胡昭1

山东大学齐鲁医院 1. 肾内科， 2. 病理科， 3. 教育部和卫生部心血管重构与功能研究重点实验室, 济南 250012

收稿日期:2009-11-17 出版日期:2010-03-16 发布日期:2010-03-16
通讯作者: 胡昭（1962- ），男，教授，主要从事慢性肾脏病的研究。
作者简介:亓敏（1983- ），女，硕士研究生，主要从事肾小球硬化的研究。
基金资助:
国家科技支撑计划课题《慢性肾脏病（CKD）预警与防治研究》（2007BAI04B10）

Effects of Niaoduqing on renal fibrosis of chronic renal failure rats

QI Min 1， LIANG Su-ren 1,　WANG Na 1,　ZHEN Jun-hui 2,　FENG Jin-bo 3,HU Zhao 1

1. Department of Nephrology； 2. Department of Pathology; 3. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Jinan 250012， China

Received:2009-11-17 Online:2010-03-16 Published:2010-03-16

摘要/Abstract

摘要：

目的探讨尿毒清对慢性肾功能衰竭大鼠肾脏纤维化的影响。方法 36只大鼠随机分为3组，正常对照组、手术对照组及尿毒清组。除正常对照组外,余均采用左侧肾脏切除加尾静脉重复注射阿霉素的方法制作慢性肾功能衰竭大鼠模型。术后1周正常对照组和手术对照组每天灌胃同体积生理盐水，尿毒清组给予尿毒清混悬液3g/（kg·d）灌胃,共给药8周。于第4、8周测量24h尿蛋白定量、肾功能，第8周光镜下观察肾脏病理变化，应用免疫组织化学SABC法测定肾组织α-平滑肌肌动蛋白(α-SMA)、胶原Ⅳ（COLIV）的表达。结果与正常对照组比较,手术对照组24 h尿蛋白、血尿素氮（Bun）、血清肌酐（SCr）明显升高(P＜0.01)，肾组织α-SMA、COLIV的表达较高(P＜0.01)。尿毒清组尿蛋白、Bun、Scr均较手术对照组明显下降,肾组织α-SMA和COLIV的表达低于手术对照组(P＜0.01)。结论尿毒清可减少蛋白尿的排泄，改善肾功能，抑制肾小管上皮细胞表型转化,延缓肾脏纤维化。

关键词: 肾脏纤维化；尿毒清；α-平滑肌肌动蛋白；胶原Ⅳ

Abstract:

Objective To observe the effect of the combination of Niaoduqing on renal fibrosis of chronic renal failure rats. Methods A total of 36 rats were allocated into 3 groups: the normal control group, the operation control group and the Niaoduqing group. The normal control group was given physiological saline and the other 2 groups were established by adriamycin and unilateral nephrectomy. The operation group and the normal control group were given normal saline. The Niaoduqing group were given Niaoduqing for 8 weeks. At 4 weeks and 8 weeks， the 24-hour urinary protein excretion and renal function were observed. At 8 weeks there was pathological change of nephridial tissue, and immunohistochemistry was assayed to measure the expressions of α-SMA and collagenIV. Results Higher expressions of α-SMA,COLIV and more severe glomerulosclerosis in the kidney(P＜0.01),　as well as more loss of renal function were observed in the operation group than in the normal control group(P＜0.01). Those changes were less severe in the Niaoduqing group than in the operation group(P＜0.01), suggesting Niaoduqing could significantly decrease expressions of α- SMA and COLIV, and also could slow down the loss of renal function. Conclusion Niaoduqing could significantly reduce urinary protein excretion, improve renal function, inhibit epithelial cell phenotypic change of renal tubules, and delay renal fibrosis.

Key words: Renal fibrosis; Niaoduqing; α-Smooth muscle actin； Collagen Ⅳ

中图分类号:

R692

亓敏1，梁素忍1，王娜1，甄军晖2，冯进波3，胡昭1. 尿毒清对慢性肾功能衰竭大鼠肾脏纤维化的影响[J]. 山东大学学报(医学版), 2010, 48(3): 57-60.

QI Min 1， LIANG Su-ren 1,　WANG Na 1,　ZHEN Jun-hui 2,　FENG Jin-bo 3,HU Zhao 1. Effects of Niaoduqing on renal fibrosis of chronic renal failure rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(3): 57-60.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	王杰1，常相帝1，李冰1，肖晓燕1，张扬1，甄军晖2，姜虹3，胡昭1. 霉酚酸酯对糖尿病大鼠肾小管上皮细胞转分化的影响[J]. 山东大学学报(医学版), 2012, 50(6): 14-19.
[2]	常相帝1，王杰1，李冰1，甄军晖2，姜虹3，胡昭1. FK506对早期糖尿病肾病大鼠足细胞损伤的保护作用[J]. 山东大学学报(医学版), 2012, 50(6): 20-25.
[3]	范例1，高洁1，刘茂静1，甄军晖2，姜虹3, 胡昭1. 肾衰宁对糖尿病大鼠肾脏损伤的保护作用[J]. 山东大学学报(医学版), 2011, 49(1): 48-52.