应用噬菌体随机十二肽库筛选hFGF7相关模拟肽

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 46-49.

应用噬菌体随机十二肽库筛选hFGF7相关模拟肽

宗宪磊^1,2，蔡景龙²，姜笃银¹，付小兵³，盛志勇³，单菲¹

1. 山东大学组织工程研究所，济南 250033； 2. 山东大学第二医院美容整形烧伤外科，济南 250033；
3. 解放军总医院第一附属医院全军创伤修复重点实验室，北京 100037

收稿日期:2009-03-30 发布日期:2009-11-16
通讯作者: 姜笃银(1963- )，男，教授，硕士生导师，主要从事创伤修复、组织工程材料基础和临床应用研究。Email：jdybs2@vip.163.com
作者简介:宗宪磊(1979- )，男，博士研究生，主要从事创面愈合与瘢痕防治研究。
基金资助:
国家自然科学基金资助课题(30670571，30772258)；国家重点基础研究发展计划(973计划)资助项目(2005CB522603)；山东省教育厅科研发展计划资助项目(J07WD03)。

hFGF7 related modeling peptide screening with phage random twelve peptide library

ZONG Xianlei ^1,2 , CAI Jinglong ² , JIANG Duyin ¹ , FU Xiaobing ³ , SHENG Zhiyong ³ , SHAN Fei ¹

1. Institute of Tissue Engineering, Shandong University, Jinan 250033, China;
2. Department of Plastic and Burn Surgery, Second Hospital of Shandong University, Jinan 250033, China;
3. Key Laboratory of Wound Repair, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China

Received:2009-03-30 Published:2009-11-16

摘要/Abstract

摘要：

目的应用噬菌体随机十二肽库生物淘选具有刺激表皮细胞增殖作用的人成纤维细胞生长因子7(hFGF7)噬菌体模拟肽。方法以hFGF7单克隆抗体为靶，进行4轮生物淘选噬菌体随机十二肽库；随机挑取单克隆噬菌体,应用ELISA方法检测单克隆噬菌体的结合力，对结合力较好的单克隆噬菌体提取噬菌体DNA，进行测序，并行序列分析。结果经过4轮生物淘选，特异性噬菌体模拟肽获得了富集，ELISA检测结果显示获得的一些噬菌体模拟肽具有较好的结合力，测序获得11个与促进细胞分裂、增殖或抑制细胞增殖有关的碱基序列。结论从噬菌体随机十二肽库中可淘选到与hFGF7相关的噬菌体模拟肽，其中部分噬菌体模拟肽可能会促进表皮细胞增殖，期望将来可应用于促进创面愈合。

关键词: 噬菌体随机十二肽库；人成纤维细胞生长因子7噬菌体模拟肽；生物淘选；测序

Abstract:

To harvest hFGF7 modeling peptides from a phage random twelve peptide library to promote epidermal cells proliferation. MethodsA phage random twelve peptide library was screened 4 times by monoclonal antihuman FGF7. ELISA staining examination was performed to choose monoclonal phages at random. Also, DNA extracted from phages with better bindingactivity was sequenced and genecompared. ResultsAfter 4 times of screening, abundant of specific phage modeling peptides were harvested. The results of ELISA showed a fine associativity of some phage modeling peptides. A total of 11 sequences were recognized which were related to promoting cell division and proliferation or inhibiting cell proliferation. ConclusionhFGF7 related phage modeling peptides can be harvested from a phage random twelve peptide library， in which some phage modeling peptides may promote epidermis cells proliferation.

Key words: Phage random twelve peptide library; Homo fibroblast growth factor7 phage modeling peptide; Screen; Sequence analysis

中图分类号:

R622

宗宪磊1,2，蔡景龙2，姜笃银1，付小兵3，盛志勇3，单菲1. 应用噬菌体随机十二肽库筛选hFGF7相关模拟肽[J]. 山东大学学报(医学版), 2009, 47(11): 46-49.

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