利奈唑胺与万古霉素对金黄色葡萄球菌耐药突变选择的比较

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (8): 42-44.

利奈唑胺与万古霉素对金黄色葡萄球菌耐药突变选择的比较

刘明涛¹ ,孙恩华² ,毕少杰¹ ,于瑞丛² ,李玉¹

山东大学齐鲁医院 1. 呼吸内科； 2. 细菌室, 济南 250012

收稿日期:2008-11-19 发布日期:2009-08-16
通讯作者: 李玉(1963- )，男，主任医师，硕导，主要从事肺部感染性疾病的研究。 Email：qlliyu@tom.com
作者简介:刘明涛(1984- )，男，硕士研究生，主要从事细菌耐药的研究。Email：842764917@qq.com

Comparison of linezolid and vancomycin in preventing the drug resistant mutants for staphylococcus aureus

LIU Mingtao ¹，　SUN Enhua²，　BI Shaojie ¹，　YU Ruicong²，　LI Yu ¹

1. Department of Pulmonary Diseases; 2. Department of Bacteria Room， Qilu Hospital of Shandong University, Jinan 250012, China

Received:2008-11-19 Published:2009-08-16

摘要/Abstract

摘要：

目的在体外测定利奈唑胺与万古霉素对金黄色葡萄球菌ATCC29213的最低抑菌浓度(MIC)、防细菌耐药突变体选择浓度(MPC)和突变选择指数(SI)，比较其防耐药突变能力。方法采用肉汤法富集1×1010?CFU/mL金黄色葡萄球菌ATCC29213，采用平板稀释法测两种药物对金黄色葡萄菌的MIC、MPC以及SI，同时测定万古霉素联合利福平与左氧氟沙星后的MPC、SI。结果利奈唑胺与万古霉素对金黄色葡萄球菌ATCC29213的MIC、MPC以及SI分别为1.0、1.0?g/L；6.4、 51.2?g/L；6.4、51.2；万古霉素分别联合利福霉素与左氧氟沙星后的MPC及SI分别为12.8?g/L、25.6；8.0?g/L、16.0。结论利奈唑胺对金黄色葡萄球菌防耐药突变能力强于万古霉素，万古霉素与利福霉素及左氧氟沙星联合后均能降低万古霉素的MPC、SI，提高万古霉素的防耐药突变能力。

关键词: 万古霉素；恶唑烷酮类；葡萄球菌,金黄色；防耐药突变浓度

Abstract:

To determine the minimum inhibitory concentration (MIC),mutant prevention concentration(MPC) and selection index(SI) of linezolid and vancomycin for staphylococcus aureus in vitro and compare their capacity for restricting the selection of staphylococcus aureus resistant mutants. MethodsThe staphylococcus aureus strain ATCC25923 was enriched in broth， and the bacterial concentration was adjusted to 1010 colony forming units per milliliter. MIC, MPC and MPC of linezolid and vancomycin for staphylococcus aureus were determined by an agar plates dilution method, We also MPC and SI of vancomycin in combination with rifamycin and leofloxacin were determined. ResultsMICs of linezolid and vancomycin for staphylococcus aureus strain ATCC25923 were 1.0 and 1.0?g/L; MPCs were 6.4 and 51.2?g/L; and SIs were 6.4 and 51.2. The MPCs of vancomycin in combination with rifamycin and leofloxacin were 12.8 and 8.0?g/L, and the SIs were 25.6 and 16.0. ConclusionThe capacity of linezolid for restricting the selection of staphylococcus aureus resistant mutants was stronger than that of vancomycin, and the capacity of vancomycin was raised by combining with rifamycin and leofloxacin.

Key words: Vancomycin; Oxazolidinones; Staphylococcus aureus; Mutant prevention concentration

中图分类号:

R978.1

. 利奈唑胺与万古霉素对金黄色葡萄球菌耐药突变选择的比较[J]. 山东大学学报(医学版), 2009, 47(8): 42-44.

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