葡萄糖浓度波动对单核细胞表达白介素6的影响

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (8): 18-20.

葡萄糖浓度波动对单核细胞表达白介素6的影响

宋华静¹，杨利波²，亓文波²

1．泰山疗养院内科，山东泰安 271000； 2．泰山医学院附属泰安医院，山东泰安 271000

收稿日期:2009-04-12 发布日期:2009-08-16
通讯作者: 亓文波(1956- )，女，教授。Email:qwb125@yahoo.com.cn
作者简介:宋华静(1977- )，女，硕士研究生，主要从事糖尿病基础与临床研究。

Effect of glucose fluctuation on IL6 produced by Monocytes

SONG Huajing^1,　YANG Libo²,　QI Wenbo²

1. Department of medicine, Taishan Sanatorium; 2. Taian Hospital Affiliated to Taishan Medical College, Taian 271000, Shandong, China

Received:2009-04-12 Published:2009-08-16

摘要/Abstract

摘要：

目的通过检测不同浓度葡萄糖及葡萄糖浓度波动对单核细胞产生白介素6（IL6）的影响，探讨血糖波动在糖尿病大血管病变发生机制中的作用。方法实验所采用的细胞为THP1细胞株，根据葡萄糖浓度及其变化分为对照组、高葡萄糖组、葡萄糖浓度波动组和渗透压控制组。将细胞接种于六孔培养板，每12?h换液1次，留取上清液。72?h后将每次留取的上清液等量混匀后采用酶联免疫法测定IL6浓度，比较各组差异有无统计学意义。结果高葡萄糖组IL6浓度明显高于对照组(P=0.000)，葡萄糖浓度波动组高于高葡萄糖组(P=0.000)，渗透压控制组介于高葡萄糖组和葡萄糖浓度波动组之间。结论葡萄糖浓度波动较单纯高糖更能促进单核细胞分泌IL6,提示葡萄糖浓度波动有更强的细胞毒性。

关键词: 单核细胞；葡萄糖；浓度波动；白介素6

Abstract:

To investigate the effect of fluctuation of glucose in diabetic macroangiopathy. MethodsThe THP1cell strain, was randomly divided into the control group, the high glucose group, the fluctuating glucose concentration group and the osmotic pressure control group. Cells were incubated in a sixbore board, the culture fluid was changed every 12?h and the clean supernatant liquid was collected. The levels of IL6 were detected by euzymelinked immunosorbent assay after 72?h. ResultThe level of IL6 in the high glucose group was significantly higher than that in the low glucose group(P=0.000).　The level of IL6 of the fluctuating glucose group was higher than that of the high glucose group(P=0.000). The concentration of IL6 of the osmotic pressure control group was between the high glucose group and the fluctuating glucose group. ConclusionFluctuation of glucose can promote monocytes secreting more IL6 and produces a greater cellular toxicity than simple high glucose does.

Key words: Ｍonocyte; Ｇlucose; Ｇlucose fluctuation; Interleukin 6

中图分类号:

R364.5

. 葡萄糖浓度波动对单核细胞表达白介素6的影响[J]. 山东大学学报(医学版), 2009, 47(8): 18-20.

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