山东汉族人群IL12B基因多态性与慢性阻塞性肺疾病的相关性

山东大学学报(医学版)

山东汉族人群IL12B基因多态性与慢性
阻塞性肺疾病的相关性

尹迎秋¹，王爱华¹，孔令敏²，张民³，马道新⁴，于钦凤¹

（1. 山东大学齐鲁医院呼吸内科，济南 250012； 2. 山东中医药大学第二附属医院职业病科, 济南 250001；3. 山东省胸科医院内五科，济南 250013； 4. 山东大学齐鲁医院血液病研究室，济南 250012）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16
通讯作者: 王爱华

Association analysis of the IL12B gene polymorphisms with chronic obstructive pulmonary disease in the Chinese Han population

YIN Yingqiu¹, WANG Aihua¹, KONG Lingmin², ZHANG Min³, MA Daoxin⁴, YU Qinfeng¹

(1. Department of Respiratory, Qilu Hospital of Shandong University, Jinan 250012, China; 2. Department of Occupation Diseases,
Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250001, China;
3. Fifth Department of Medicine, Shandong Provincial Chest Hospital, Jinan 250013, China;
4. Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16
Contact: WANG Aihua

摘要/Abstract

摘要： 目的探讨白细胞介素12B(IL12B)基因1188A/C位点多态性与山东汉族人群慢性阻塞性肺疾病（COPD）易感性的关系。方法采用PCRRFLP法对324例COPD患者和389例健康人IL12B基因1188A/C位点多态性进行分型，对等位基因及基因型分布进行统计学分析。结果IL12B基因1188A/C多态位点AA、AC、CC基因型频率在病例组中分别为0.333?3、0.478?4和0.188?3，在对照组中分别为0.357?3、0.475?6和0.167?1，A和C等位基因型的频率在病例组中分别为0.572?5和0.427?5，在对照组分别为0.595?1和0.404?9，该位点基因型和等位基因型分布在病例组与对照组P值分别为0.689，0.389，差异均无统计学意义。结论IL12B基因1188A/C位点多态性与山东汉族人群COPD发生无明显相关性。

关键词: 白细胞介素12, 肺疾病,慢性阻塞性, 疾病遗传易感性

Abstract: To explore the relationship between single nuclear polymorphism (SNP) of 1188A/C in the interleukin 12B gene and susceptibility of chronic obstructive pulmonary disease (COPD) in the Chinese Han population from Shandong Province. MethodsSNP 1188A/C of the IL12B gene was detected by polymerase chain reaction (PCR) based on the restriction fragment length polymorphism (RFLP) technique in 324 COPD subjects and 389 healthy controls. ResultsThe frequencies of genotypes AA, AC, and CC at polymorphism sites 1188A/C were 0.333?3, 0.478?4, and 0.188?3 respectively in the COPD group, while they were 0.357?3, 0.475?6, and 0.167?1 in the control group. Also, the A type allele frequencies were 0.572?5 and 0.595?1 in patients and controls, and the C type allele frequency was 0.427?5 and 0.404?9 in COPD patients and healthy controls. There were no significant differences in genotype frequencies (P value=0.689) or allele frequencies between the two groups (P=0.389). ConclusionNo significant association was found between IL12B 1188A/C polymorphism and COPD susceptibility in the Chinese Han population from Shandong Province.

Key words: Interleukin12, Pulmonary disease,chronic obstructive, Genetic predisposition to disease

中图分类号:

R563

尹迎秋1 ，王爱华1 ，孔令敏2 ，张民3 ，马道新4 ，于钦凤1
. 山东汉族人群IL12B基因多态性与慢性
阻塞性肺疾病的相关性[J]. 山东大学学报(医学版), 2009, 47(02): 38-41.

YIN Yingqiu¹, WANG Aihua¹, KONG Lingmin², ZHANG Min³, MA Daoxin⁴, YU Qinfeng¹
. Association analysis of the IL12B gene polymorphisms with chronic obstructive pulmonary disease in the Chinese Han population[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 38-41.

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