关键词: 垂体腺瘤；过氧化物酶体增殖激活物受体γ；白细胞分化抗原147；转化生长因子β1；基质金属蛋白酶9；GH3细胞

Abstract:

Objective   To explore the effect of troglitazone(TGZ), a ligand for peroxisome proliferator-activated receptor γ(PPAR-γ), on the expressions of cluster of differentiation 147(CD147), transforming growth factor beta 1(TGF-β1) and matrix metalloproteinase 9(MMP-9) in rat GH-secreting GH3 cells. Methods   GH3 cells were devided into control group and experimental groups (1×10-3, 1×10-4 and 1×10-5 mol／L TGZ). After treated with TG2 for 48 hours, expressions of CD147, TGF-β1 and MMP-9 at protein and mRNA levels were detected by Western blot and Real-time PCR respectively. Results   The expressions of CD147, TGF-β1 and MMP-9 at protein and mRNA levels in all the TGZ treated groups showed significant differecnces compared with control group(all P<0.05). Moreover, expressions of the above three indicators decreased both at protein and mRNA levels when TGZ concentration was increased. Conclusion   TGZ can inhibit expressions of CD147, TGF-β1 and MMP-9, which   might relieve the invasiveness of GH3 cells.

Key words:  Pituitary adnoma； Peroxisome proliferators activated receptor γ； Cluster of differentiation 147； Transforming growth factor beta 1；  Matrix metalloproteinase 9； GH3 cells